Rectal prolapse is a common condition in laboratory-housed macaques, usually associated with recurrent gastroenteritis and stress. However, evidence for both statements is lacking. Therefore, the prevalence of rectal prolapses and their risk factors in laboratory-housed macaques have yet to be assessed. In addition, a standard of care pharmacological treatment remains to be recognized and documented. Thus, a retrospective study involving 816 laboratory-housed macaques was conducted, in which the prevalence, risk factors, and treatment success of rectal prolapses was assessed. The prevalence was shown to be 3.92%, and all cases could be either directly or indirectly linked to stress factors. By eliminating the suggested stressor, most rectal prolapses reverted naturally (69%). Moreover, we discuss the advantage of the ancillary administration of hyoscine butylbromide and metamizole as a treatment modality.