Grasspea (Lathyrus sativus L.) is recognized as a highly drought-tolerant legume. However, excessive consumption of its seeds and green tissues causes neurolathyrism, a condition characterized by an irreversible paralysis of the legs induced by a neurotoxin amino acid called β-N-oxalyl-L-α, β- diaminopropionic acid (β-ODAP). The present study investigated the effects of heat, and combined heat + drought during the reproductive phase on physiological and phenological parameters, yield-related factors, ODAP content, and seed protein of 24 genotypes representing 11 Lathyrus species under controlled conditions. Analysis of variance revealed a highly significant effect (p < 0.001) of stress treatments and genotypes for all the traits. In general, heat stress individually or in combination with drought expedited phenology, reduced relative leaf water content, stimulated proline synthesis, and influenced chlorophyll concentration; the effects were more severe under the combined heat + drought stress. ODAP content in seeds ranged from 0.06 to 0.30% under no-stress conditions. However, under heat stress, there was a significant increase of 33% in ODAP content, and under combined stress (heat + drought), the increase reached 83%. Crude protein content ranged from 15.64 to 28.67% among no stress plants and decreased significantly by 23% under heat stress and by 36% under combined stress. The findings of this study also indicated substantial reductions in growth and grain yield traits under both heat stress and combined heat + drought stress. Six accessions namely IG 66026, IG 65018, IG 65687, IG 118511, IG 64931, and IG65273 were identified as having the most favorable combination of yield, protein content, and seed ODAP levels across all conditions. ODAP content in these six accessions varied from 0.07 to 0.11% under no stress and remained at moderate levels during both heat stress (0.09–0.14%) and combined stress (0.11–0.17%). IG 66026 was identified as the most stable genotype under drought and heat stress conditions with high protein content, and low ODAP content. By identifying those promising accessions, our results have established a basis for forthcoming grasspea breeding initiatives while paving the way for future research exploration into the fundamental mechanisms driving ODAP variation in the presence of both heat and drought stress conditions.

Climate change, drought, erosion, water contamination resources, desertification, and loss of soil quality represent major environmental risks worldwide. Facing these risks is the most important issue for sustainable development. Conventional tillage (CT) practices seem to expose the soils of semi-arid regions, which are initially fragile, to degradation that is accentuated by the overuse of the environment. The benefits of conservation agriculture (CA) could mitigate the degradation of natural resources, particularly soils. The adaptation and transfer of the no-till (NT) system with mulch open new perspectives for the development of agriculture in semi-arid regions. The main objective of this study is to assess the impact of conservation agriculture, especially no-till (NT) system, on the physical properties of soil (structural stability (SS), bulk density (BD), gravimetric water content (θg), and soil organic matter (SOM)) compared to conventional tillage (CT). The main changes associated with the transition from a CT system to an NT system were evaluated at the experimental site, Merchouch (M13), which is typified by vertisol soil, and at the Ain Sbit (AS7) site, which is characterized by isohumic soil. Under a no-till system, most of the physical properties of the soil were improved in both sites, with a clear difference in the M13 site. Structural stability under NT showed a significant increase in both sites (fast wetting (FW), slow wetting (SW), and wet stirring (WS) improved by 88, 43, and 83% at the M13 site, respectively, against, 16, 23, and 7%, respectively, at the AS7 site). On the other hand, the SOM increased from 2.0 to 2.6% (an improvement of 28%) at AS7 and from 1.2% to 1.9% (an improvement of 52%) at M13. This research demonstrated that conservation agriculture, especially NT, improves the soil physical quality in both medium and long terms, confirming its suitability for the climatic and edaphic constraints of semi-arid areas in Morocco as well as in other parts of the world.

The use of barley (Hordeum vulgare L.) in Morocco is still limited to food and feed despite the amplified demand by local industries for imported malt. This study aims to evaluate 36 barley elite lines for major grain physicochemical parameters and malt quality traits. Analysis of variance, Pearson correlation, principal component analysis (PCA), and hierarchical cluster analysis (HCA) were performed. The results showed significant genotypic variation among genotypes for individual grain and malt traits. High broad sense heritability was obtained for all traits except for plump grain percentage, malt friability, and germination capacity. Starch, malt extract, Kolbach index, grain area, and test weight correlated significantly and negatively with barley protein. Malt extract correlated positively with Kolbach index and starch, but a negative correlation with soluble protein and malt protein was found. Based on 12 characters, 77% of the total genotypic variation was explained by the three first principal components following PCA and four clusters were depicted based on HCA. Genotypes of high interest with desirable levels of quality standards were identified to be used as a malt quality traits donor while designing crossing programs. Supplemental data for this article is available online at https://doi.org/10.1080/03610470.2021.1978036 .

Grasspea (Lathyrus sativus L.) is a climate-smart legume crop with adaptation to fragile agroecosystems. The genus Lathyrus is recognized for its vast genetic diversity, encompassing over 160 species, many of which are cultivated for various purposes across different regions of the world. Among these, Lathyrus sativus is widely cultivated as food, feed, and fodder in South Asia, Sub-Saharan Africa, and the Central and West Asia and North Africa (CWANA) regions. Its global cultivation has declined substantially due to the stigma posed by the presence of neurotoxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP) in its seeds and green foliage. Overconsumption for a longer period of grasspea seeds harvested from landraces may lead to a neurological disorder called neurolathyrism in humans. ODAP is an obstacle for grasspea expansion, but crop wild relatives (CWRs) have been found to offer a solution. The incorporation of CWRs, particularly Lathyrus cicera, and landraces into breeding programs may reduce the ODAP content in grasspea varieties to a safer level. Recent advances in genomics-assisted breeding have expanded the potential for utilizing challenging CWRs to develop grasspea varieties that combine ultra-low ODAP levels with improved yield, stability, and adaptability. Further progress in omics technologies—such as transcriptomics, proteomics, and metabolomics—along with genome sequencing and editing, has greatly accelerated the development of grasspea varieties with reduced or zero ODAP content, while also enhancing the plant’s agronomic value. This review highlights the significance of utilizing CWRs in pre-breeding programs, and harnessing advanced tools and technologies to enhance the performance, adaptability, and resilience of grasspea in response to changing environmental conditions.

Management of broomrape (Orobanche crenata Fosk.) that causes important damages on lentil production becomes a veritable concern in the Mediterranean region. Eighty lentil accessions were evaluated for resistance to O. crenata under field and controlled conditions. Both genotypes ILL6415 and ILL7723 expressed the highest resistance level under field and pot experiment with low Orobanche infestation and relatively high seed yield (50.1 g m⁻²). Such resistance was associated with physiological and biochemical changes in metabolites profiling. In total, 109 and 115 metabolites were identified in the lipophilic phase of both ILL6415 and ILL7723, respectively, against only 92 metabolites recorded for susceptible check Zaaria. Significant differences were observed in metabolite concentrations (fatty acids, sterols alkanes) between roots and shoots of susceptible and resistant infested plants. Accumulation of α-linolenic acid and arachidic acid was more pronounced in the resistant genotypes ILL6415, ILL7723 which could be associated with resistance pathways involved in the resistance to O. crenata. GRAPHICAL ABSTRACT