Strawberry (Fragaria X ananassa Duch.) can be infected with multiple viruses in single or mixed infections producing severe symptoms and significant yield reduction. Among aphid-borne viruses, Strawberry mild yellow edge virus (SMYEV), Strawberry crinkle virus (SCV) and Strawberry mottle virus (SMoV) have been detected in Argentina. Strawberry plants with dwarfism, general weakness, distorted leaves, occasional chlorosis, few flowers and poor fruit quality are observed often in Argentina. The productivity of these affected plants is greatly reduced. Symptomatic plants from Tucumán, Argentina (26°55′22″S 65°20′15″W) were collected from the field and maintained in a greenhouse. Total RNA was extracted from leaves using an RNeasy Plant Mini Kit (QIAGEN, Valencia, CA) according to manufacturer’s instructions, and subjected to next generation sequencing (NGS) at the Genomics and Bioinformatics Platform, INDEAR Inc., Rosario, Argentina using Roche 454 Genome Sequencer FLX+. The sequence reads were quality filte...

Virus species of the genus Orthotospovirus are among the most economically important plant pathogens in the world because they cause severe crop losses; mainly, in ornamental and horticultural crop...