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Prof. Agostinho Antunes
1. CIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixoes, Av. General Norton de Matos, s/n, 4450–208 Porto, Portugal </br>2. Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal

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0 Bioinformatics
0 Biotechnology
0 Conservation
0 Genomics (from animals to microorganisms)
0 Evolution, molecular ecology

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Review
Published: 26 June 2021 in Marine Drugs
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Associations between different organisms have been extensively described in terrestrial and marine environments. These associations are involved in roles as diverse as nutrient exchanges, shelter or adaptation to adverse conditions. Ascidians are widely dispersed marine invertebrates associated to invasive behaviours. Studying their microbiomes has interested the scientific community, mainly due to its potential for bioactive compounds production—e.g., ET-73 (trabectedin, Yondelis), an anticancer drug. However, these symbiotic interactions embrace several environmental and biological functions with high ecological relevance, inspiring diverse biotechnological applications. We thoroughly reviewed microbiome studies (microscopic to metagenomic approaches) of around 171 hosts, worldwide dispersed, occurring at different domains of life (Archaea, Bacteria, Eukarya), to illuminate the functions and bioactive potential of associated organisms in ascidians. Associations with Bacteria are the most prevalent, namely with Cyanobacteria, Proteobacteria, Bacteroidetes, Actinobacteria and Planctomycetes phyla. The microbiomes of ascidians belonging to Aplousobranchia order have been the most studied. The integration of worldwide studies characterizing ascidians’ microbiome composition revealed several functions including UV protection, bioaccumulation of heavy metals and defense against fouling or predators through production of natural products, chemical signals or competition. The critical assessment and characterization of these communities is extremely valuable to comprehend their biological/ecological role and biotechnological potential.

ACS Style

Ana Matos; Agostinho Antunes. Symbiotic Associations in Ascidians: Relevance for Functional Innovation and Bioactive Potential. Marine Drugs 2021, 19, 370 .

AMA Style

Ana Matos, Agostinho Antunes. Symbiotic Associations in Ascidians: Relevance for Functional Innovation and Bioactive Potential. Marine Drugs. 2021; 19 (7):370.

Chicago/Turabian Style

Ana Matos; Agostinho Antunes. 2021. "Symbiotic Associations in Ascidians: Relevance for Functional Innovation and Bioactive Potential." Marine Drugs 19, no. 7: 370.

Journal article
Published: 10 June 2021 in Virus Research
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The human β-coronavirus SARS-CoV-2 epidemic started in late December 2019 in Wuhan, China. It causes Covid-19 disease which has become pandemic. Each of the five-known human β-coronaviruses has four major structural proteins (E, M, N and S) and 16 non-structural proteins encoded by ORF1a and ORF1b together (ORF1ab) that are involved in virus pathogenicity and infectivity. Here, we performed detailed positive selection analyses for those six genes among the four previously known human β-coronaviruses and within 38 SARS-CoV-2 genomes to assess signatures of adaptive evolution using maximum likelihood approaches. Our results suggest that three genes (E, S and ORF1ab genes) are under strong signatures of positive selection among human β-coronavirus, influencing codons that are located in functional important protein domains. The E protein-coding gene showed signatures of positive selection in two sites, Asp 66 and Ser 68, located inside a putative transmembrane α-helical domain C-terminal part, which is preferentially composed by hydrophilic residues. Such Asp and Ser sites substitutions (hydrophilic residues) increase the stability of the transmembrane domain in SARS-CoV-2. Moreover, substitutions in the spike (S) protein S1 N-terminal domain have been found, all of them were located on the S protein surface, suggesting their importance in viral transmissibility and survival. Furthermore, evidence of strong positive selection was detected in three of the SARS-CoV-2 nonstructural proteins (NSP1, NSP3, NSP16), which are encoded by ORF1ab and play vital roles in suppressing host translation machinery, viral replication and transcription and inhibiting the host immune response. These results are insightful to assess the role of positive selection in the SARS-CoV-2 encoded proteins, which will allow to better understand the virulent pathogenicity of the virus and potentially identifying targets for drug or vaccine strategy design

ACS Style

Mohamed Emam; Mariam Oweda; Agostinho Antunes; Mohamed El-Hadidi. Positive Selection as a Key Player for SARS-CoV-2 Pathogenicity: Insights into ORF1ab, S and E genes. Virus Research 2021, 302, 198472 .

AMA Style

Mohamed Emam, Mariam Oweda, Agostinho Antunes, Mohamed El-Hadidi. Positive Selection as a Key Player for SARS-CoV-2 Pathogenicity: Insights into ORF1ab, S and E genes. Virus Research. 2021; 302 ():198472.

Chicago/Turabian Style

Mohamed Emam; Mariam Oweda; Agostinho Antunes; Mohamed El-Hadidi. 2021. "Positive Selection as a Key Player for SARS-CoV-2 Pathogenicity: Insights into ORF1ab, S and E genes." Virus Research 302, no. : 198472.

Review
Published: 11 May 2021 in Applied Sciences
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Cyanobacteria have long been associated with harmful effects on humans, animals and aquatic biota. Cyanotoxins are their most toxic metabolite. This review summarizes the current research, impacts and future needs in cyanobacterial studies undertaken in Portugal, the southernmost country of Europe, and with a recent multiplication of cyanotoxicity due to climate change events. Microcystins are still the most prevalent, studied and the only regulated cyanotoxins in Portuguese freshwater systems much like most European countries. With the development of some tools, particularly in molecular studies, the recent discovery of cylindrospermopsins, anatoxins and saxitoxins, both genes and toxins, in North and Center ecosystems of our country highlight current impacts that overall communities are facing with increased risks of exposure and uptake to cyanotoxins. Research needs encompass the expansion of studies at all aspects due to the uprising of these cyanotoxins and reinforces the urgent need of increasing the frequency of surveillance to achieve tangible effects of cyanotoxins in Portugal to ultimately implement regulations on cylindrospermopsins, anatoxins and saxitoxins worldwide.

ACS Style

Cristiana Moreira; Alexandre Campos; José Martins; Vitor Vasconcelos; Agostinho Antunes. Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs. Applied Sciences 2021, 11, 4355 .

AMA Style

Cristiana Moreira, Alexandre Campos, José Martins, Vitor Vasconcelos, Agostinho Antunes. Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs. Applied Sciences. 2021; 11 (10):4355.

Chicago/Turabian Style

Cristiana Moreira; Alexandre Campos; José Martins; Vitor Vasconcelos; Agostinho Antunes. 2021. "Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs." Applied Sciences 11, no. 10: 4355.

Journal article
Published: 26 April 2021 in Current Topics in Medicinal Chemistry
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Background: Molecular phylogenetic algorithms frequently disagree with the approaches considering reproductive compatibility and morphological criteria for species delimitation. The question stems if the resulting species boundaries from molecular, reproductive and/or morphological data are definitively not reconcilable; or if the existing phylogenetic methods are not sensitive enough to agree morphological and genetic variation in species delimitation. Objectives : We propose DISTATIS as an integrative framework to combine alignment-based (AB) and alignment-free (AF) distance matrices from ITS2 sequences/structures to shed light whether Gelasinospora and Neurospora are sister but independent genera. Methods: We aimed at addressing this standing issue by harmonizing genus-specific classification based on their ascospore morphology and ITS2 molecular data. To validate our proposal, three phylogenetic approaches: i) traditional alignment-based, ii) alignment-free and iii) novel distance integrative (DI)-based were comparatively evaluated on a set of Gelasinospora and Neurospora species. All considered species have been extensively characterized at both the morphological and reproductive levels and there are known incongruences between their ascospore morphology and molecular data that hampers genus-specific delimitation. Results: Traditional AB phylogenetic analyses fail at resolving the Gelasinospora and Neurospora genera into independent monophyletic clades following ascospore morphology criteria. In contrast, AF and DI approaches produced phylogenetic trees that could properly delimit the expected monophyletic clades. Conclusions: The DI approach outperformed the AF one in the sense that it could also divide the Neurospora species according to their reproduction mode.

ACS Style

Guillermin Agüero-Chapin; Yuliana Jiménez; Aminael Sánchez-Rodríguez; Reinaldo Molina-Ruiz; Oscar Vivanco; Agostinho Antunes. DISTATIS: A Promising Framework to Integrate Distance Matrices in Molecular Phylogenetics. Current Topics in Medicinal Chemistry 2021, 21, 599 -611.

AMA Style

Guillermin Agüero-Chapin, Yuliana Jiménez, Aminael Sánchez-Rodríguez, Reinaldo Molina-Ruiz, Oscar Vivanco, Agostinho Antunes. DISTATIS: A Promising Framework to Integrate Distance Matrices in Molecular Phylogenetics. Current Topics in Medicinal Chemistry. 2021; 21 (7):599-611.

Chicago/Turabian Style

Guillermin Agüero-Chapin; Yuliana Jiménez; Aminael Sánchez-Rodríguez; Reinaldo Molina-Ruiz; Oscar Vivanco; Agostinho Antunes. 2021. "DISTATIS: A Promising Framework to Integrate Distance Matrices in Molecular Phylogenetics." Current Topics in Medicinal Chemistry 21, no. 7: 599-611.

Journal article
Published: 25 March 2021 in GigaScience
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Venom research is a highly multidisciplinary field that involves multiple subfields of biology, informatics, pharmacology, medicine, and other areas. These different research facets are often technologically challenging and pursued by different teams lacking connection with each other. This lack of coordination hampers the full development of venom investigation and applications. The COST Action CA19144–European Venom Network was recently launched to promote synergistic interactions among different stakeholders and foster venom research at the European level.

ACS Style

Maria Vittoria Modica; Rafi Ahmad; Stuart Ainsworth; Gregor Anderluh; Agostinho Antunes; Dimitris Beis; Figen Caliskan; Mauro Dalla Serra; Sebastien Dutertre; Yehu Moran; Ayse Nalbantsoy; Naoual Oukkache; Stano Pekar; Maido Remm; Bjoern Marcus von Reumont; Yiannis Sarigiannis; Andrea Tarallo; Jan Tytgat; Eivind Andreas Baste Undheim; Yuri Utkin; Aida Verdes; Aude Violette; Giulia Zancolli. The new COST Action European Venom Network (EUVEN)—synergy and future perspectives of modern venomics. GigaScience 2021, 10, 1 .

AMA Style

Maria Vittoria Modica, Rafi Ahmad, Stuart Ainsworth, Gregor Anderluh, Agostinho Antunes, Dimitris Beis, Figen Caliskan, Mauro Dalla Serra, Sebastien Dutertre, Yehu Moran, Ayse Nalbantsoy, Naoual Oukkache, Stano Pekar, Maido Remm, Bjoern Marcus von Reumont, Yiannis Sarigiannis, Andrea Tarallo, Jan Tytgat, Eivind Andreas Baste Undheim, Yuri Utkin, Aida Verdes, Aude Violette, Giulia Zancolli. The new COST Action European Venom Network (EUVEN)—synergy and future perspectives of modern venomics. GigaScience. 2021; 10 (3):1.

Chicago/Turabian Style

Maria Vittoria Modica; Rafi Ahmad; Stuart Ainsworth; Gregor Anderluh; Agostinho Antunes; Dimitris Beis; Figen Caliskan; Mauro Dalla Serra; Sebastien Dutertre; Yehu Moran; Ayse Nalbantsoy; Naoual Oukkache; Stano Pekar; Maido Remm; Bjoern Marcus von Reumont; Yiannis Sarigiannis; Andrea Tarallo; Jan Tytgat; Eivind Andreas Baste Undheim; Yuri Utkin; Aida Verdes; Aude Violette; Giulia Zancolli. 2021. "The new COST Action European Venom Network (EUVEN)—synergy and future perspectives of modern venomics." GigaScience 10, no. 3: 1.

Journal article
Published: 25 March 2021 in International Journal of Molecular Sciences
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We focus on the stalked goose barnacle L. anatifera adhesive system, an opportunistic less selective species for the substrate, found attached to a variety of floating objects at seas. Adhesion is an adaptative character in barnacles, ensuring adequate positioning in the habitat for feeding and reproduction. The protein composition of the cement multicomplex and adhesive gland was quantitatively studied using shotgun proteomic analysis. Overall, 11,795 peptide sequences were identified in the gland and 2206 in the cement, clustered in 1689 and 217 proteinGroups, respectively. Cement specific adhesive proteins (CPs), proteases, protease inhibitors, cuticular and structural proteins, chemical cues, and many unannotated proteins were found, among others. In the cement, CPs were the most abundant (80.5%), being the bulk proteins CP100k and -52k the most expressed of all, and CP43k-like the most expressed interfacial protein. Unannotated proteins comprised 4.7% of the cement proteome, ranking several of them among the most highly expressed. Eight of these proteins showed similar physicochemical properties and amino acid composition to known CPs and classified through Principal Components Analysis (PCA) as new CPs. The importance of PCA on the identification of unannotated non-conserved adhesive proteins, whose selective pressure is on their relative amino acid abundance, was demonstrated.

ACS Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André Machado; Lothar Jänsch; Agostinho Antunes; Luís Castro; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera. International Journal of Molecular Sciences 2021, 22, 3370 .

AMA Style

Dany Domínguez-Pérez, Daniela Almeida, Josef Wissing, André Machado, Lothar Jänsch, Agostinho Antunes, Luís Castro, Vitor Vasconcelos, Alexandre Campos, Isabel Cunha. Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera. International Journal of Molecular Sciences. 2021; 22 (7):3370.

Chicago/Turabian Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André Machado; Lothar Jänsch; Agostinho Antunes; Luís Castro; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. 2021. "Proteogenomic Characterization of the Cement and Adhesive Gland of the Pelagic Gooseneck Barnacle Lepas anatifera." International Journal of Molecular Sciences 22, no. 7: 3370.

Research article
Published: 20 February 2021 in Environmental Science and Pollution Research
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Cyanobacteria are a group of microorganisms that can be found in a diverse range of biogeographical areas and produce potent and damaging cyanotoxins, which reveal importance for continuous studies and surveillance efforts. In this study, we analyzed worldwide two-month culture-enriched water samples collected from 12 distinct countries (Costa Rica, Cuba, Fiji, France, Indonesia, Mali, Portugal, South Africa, Spain, Thailand, USA, Vietnam) including two undisclosed areas (Fiji and Mali). We performed a PCR-based molecular multi-step scheme that consisted in the detection of the main cyanobacterial species, genera, and cyanotoxins biosynthesis genes. Results from this study indicate that Microcystis aeruginosa followed by Planktothrix agardhii were the most prevalent species of all the 12 countries analyzed. Cylindrospermospis raciborskii was detected in Costa Rica, while P. agardhii was detected in Fiji and South Africa. M. aeruginosa was detected in Fiji and Mali. Regarding the main cyanotoxins biosynthesis genes, a cyrC gene fragment (cylindrospermopsins) was amplified in the African continent (South Africa), while anaC (anatoxin-a) was detected in two distinct locations, Mali and Vietnam. Saxitoxins biosynthesis gene was also detected in Fiji and Vietnam. Microcystins biosynthesis gene (mcyA) was co-detected with anatoxin-a biosynthesis gene in Mali and with saxitoxins biosynthesis gene (sxtI) in Portugal. This study therefore constitutes a major contribution to the global biogeography of cyanobacteria and its cyanotoxins and recommends continuous vigilance of toxic cyanobacteria particularly in the more undisclosed areas of the world. The PCR analysis data obtained in our 2-month culture-enriched water samples supports molecular methods as a preliminary tool in the environmental surveillance of cyanobacteria and cyanotoxins in undisclosed locations, particularly since the several positive amplifications detected may indicate that though samples were collected under non-bloom conditions, if environmental conditions change in the ecosystem, there is a risk that bloom-forming species may arose along with their detected cyanotoxicity.

ACS Style

Cristiana Moreira; Ana Pimentel; Vitor Vasconcelos; Agostinho Antunes. Preliminary evidence on the presence of cyanobacteria and cyanotoxins from culture enrichments followed by PCR analysis: new perspectives from Africa (Mali) and South Pacific (Fiji) countries. Environmental Science and Pollution Research 2021, 28, 31731 -31745.

AMA Style

Cristiana Moreira, Ana Pimentel, Vitor Vasconcelos, Agostinho Antunes. Preliminary evidence on the presence of cyanobacteria and cyanotoxins from culture enrichments followed by PCR analysis: new perspectives from Africa (Mali) and South Pacific (Fiji) countries. Environmental Science and Pollution Research. 2021; 28 (24):31731-31745.

Chicago/Turabian Style

Cristiana Moreira; Ana Pimentel; Vitor Vasconcelos; Agostinho Antunes. 2021. "Preliminary evidence on the presence of cyanobacteria and cyanotoxins from culture enrichments followed by PCR analysis: new perspectives from Africa (Mali) and South Pacific (Fiji) countries." Environmental Science and Pollution Research 28, no. 24: 31731-31745.

Technical review
Published: 06 February 2021 in Conservation Genetics Resources
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ACS Style

Rodrigo Rodrigues Domingues; Ingrid Vasconcellos Bunholi; Danillo Pinhal; Agostinho Antunes; Fernando Fernandes Mendonça. From molecule to conservation: DNA-based methods to overcome frontiers in the shark and ray fin trade. Conservation Genetics Resources 2021, 13, 231 -247.

AMA Style

Rodrigo Rodrigues Domingues, Ingrid Vasconcellos Bunholi, Danillo Pinhal, Agostinho Antunes, Fernando Fernandes Mendonça. From molecule to conservation: DNA-based methods to overcome frontiers in the shark and ray fin trade. Conservation Genetics Resources. 2021; 13 (2):231-247.

Chicago/Turabian Style

Rodrigo Rodrigues Domingues; Ingrid Vasconcellos Bunholi; Danillo Pinhal; Agostinho Antunes; Fernando Fernandes Mendonça. 2021. "From molecule to conservation: DNA-based methods to overcome frontiers in the shark and ray fin trade." Conservation Genetics Resources 13, no. 2: 231-247.

Data descriptor
Published: 27 November 2020 in Data
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Here we provide all datasets and details applied in the construction of a composite protein database required for the proteogenomic analyses of the article “Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database”. All data, subdivided into six datasets, are deposited at the Mendeley Data repository as follows. Dataset_1 provides our composite database “All_Databases_5950827_sequences.fasta” derived from six smaller databases composed of (i) protein sequences retrieved from public databases related to cephalopods’ salivary glands, (ii) proteins identified with Proteome Discoverer software using our original data obtained by shotgun proteomic analyses of posterior salivary glands (PSGs) from three Octopus vulgaris specimens (provided as Dataset_2) and (iii) a non-redundant antimicrobial peptide (AMP) database. Dataset_3 includes the transcripts obtained by de novo assembly of 16 transcriptomes from cephalopods’ PSGs using CLC Genomics Workbench. Dataset_4 provides the proteins predicted by the TransDecoder tool from the de novo assembly of 16 transcriptomes of cephalopods’ PSGs. Further details about database construction, as well as the scripts and command lines used to construct them, are deposited within Dataset_5 and Dataset_6. The data provided in this article will assist in unravelling the role of cephalopods’ PSGs in feeding strategies, toxins and AMP production.

ACS Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Yuselis Castaño; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. Data Employed in the Construction of a Composite Protein Database for Proteogenomic Analyses of Cephalopods Salivary Apparatus. Data 2020, 5, 110 .

AMA Style

Daniela Almeida, Dany Domínguez-Pérez, Ana Matos, Guillermin Agüero-Chapin, Yuselis Castaño, Vitor Vasconcelos, Alexandre Campos, Agostinho Antunes. Data Employed in the Construction of a Composite Protein Database for Proteogenomic Analyses of Cephalopods Salivary Apparatus. Data. 2020; 5 (4):110.

Chicago/Turabian Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Yuselis Castaño; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. 2020. "Data Employed in the Construction of a Composite Protein Database for Proteogenomic Analyses of Cephalopods Salivary Apparatus." Data 5, no. 4: 110.

Research articles
Published: 05 November 2020 in FEBS Letters
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Enzyme subunit interfaces have remarkable potential in drug design as both target and scaffold for their own inhibitors. We show an evolution‐driven strategy for the de novo design of peptide inhibitors targeting interfaces of the Escherichia coli FoF1‐ATP synthase as a case study. The evolutionary algorithm ROSE was applied to generate diversity‐oriented peptide libraries by engineering peptide fragments from ATP synthase interfaces. The resulting peptides were scored with PPI‐Detect, a sequence‐based predictor of protein‐protein interactions. Two selected peptides were confirmed by in vitro inhibition and binding tests. The proposed methodology can be widely applied to design peptides targeting relevant interfaces of enzymatic complexes.

ACS Style

Yasser B. Ruiz‐Blanco; Luis Pablo Ávila‐Barrientos; Enrique Hernández‐García; Agostinho Antunes; Guillermin Agüero‐Chapin; Enrique García‐Hernández. Engineering protein fragments via evolutionary and protein–protein interaction algorithms: de novo design of peptide inhibitors for F O F 1 ‐ATP synthase. FEBS Letters 2020, 595, 183 -194.

AMA Style

Yasser B. Ruiz‐Blanco, Luis Pablo Ávila‐Barrientos, Enrique Hernández‐García, Agostinho Antunes, Guillermin Agüero‐Chapin, Enrique García‐Hernández. Engineering protein fragments via evolutionary and protein–protein interaction algorithms: de novo design of peptide inhibitors for F O F 1 ‐ATP synthase. FEBS Letters. 2020; 595 (2):183-194.

Chicago/Turabian Style

Yasser B. Ruiz‐Blanco; Luis Pablo Ávila‐Barrientos; Enrique Hernández‐García; Agostinho Antunes; Guillermin Agüero‐Chapin; Enrique García‐Hernández. 2020. "Engineering protein fragments via evolutionary and protein–protein interaction algorithms: de novo design of peptide inhibitors for F O F 1 ‐ATP synthase." FEBS Letters 595, no. 2: 183-194.

Journal article
Published: 30 October 2020 in Antibiotics
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Cephalopods, successful predators, can use a mixture of substances to subdue their prey, becoming interesting sources of bioactive compounds. In addition to neurotoxins and enzymes, the presence of antimicrobial compounds has been reported. Recently, the transcriptome and the whole proteome of the Octopus vulgaris salivary apparatus were released, but the role of some compounds—e.g., histones, antimicrobial peptides (AMPs), and toxins—remains unclear. Herein, we profiled the proteome of the posterior salivary glands (PSGs) of O. vulgaris using two sample preparation protocols combined with a shotgun-proteomics approach. Protein identification was performed against a composite database comprising data from the UniProtKB, all transcriptomes available from the cephalopods’ PSGs, and a comprehensive non-redundant AMPs database. Out of the 10,075 proteins clustered in 1868 protein groups, 90 clusters corresponded to venom protein toxin families. Additionally, we detected putative AMPs clustered with histones previously found as abundant proteins in the saliva of O. vulgaris. Some of these histones, such as H2A and H2B, are involved in systemic inflammatory responses and their antimicrobial effects have been demonstrated. These results not only confirm the production of enzymes and toxins by the O. vulgaris PSGs but also suggest their involvement in the first line of defense against microbes.

ACS Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Hugo Osório; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database. Antibiotics 2020, 9, 757 .

AMA Style

Daniela Almeida, Dany Domínguez-Pérez, Ana Matos, Guillermin Agüero-Chapin, Hugo Osório, Vitor Vasconcelos, Alexandre Campos, Agostinho Antunes. Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database. Antibiotics. 2020; 9 (11):757.

Chicago/Turabian Style

Daniela Almeida; Dany Domínguez-Pérez; Ana Matos; Guillermin Agüero-Chapin; Hugo Osório; Vitor Vasconcelos; Alexandre Campos; Agostinho Antunes. 2020. "Putative Antimicrobial Peptides of the Posterior Salivary Glands from the Cephalopod Octopus vulgaris Revealed by Exploring a Composite Protein Database." Antibiotics 9, no. 11: 757.

Journal article
Published: 01 October 2020 in Journal of Urban Health
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High levels of population differentiation are a common demographic pattern in syngnathids, even at small geographical scales. This is probably the end result of the common life history traits observed within the family, involving limited dispersal capabilities and strong habitat dependency. The worm pipefish, Nerophis lumbriciformis, which displays all these characteristics, also presents an additional variable potentially able to promote population differentiation: high sexual selection intensity, especially at the extremes of its distribution. Nevertheless, an early life pelagic stage, which presumably allows for admixture, could prevent population structuring. Here, we assessed the phylogeography of N. lumbriciformis through the amplification of the cytochrome b, 12S, and 16S rDNA mitochondrial markers as well as the rhodopsin nuclear marker, performed upon 119 individuals. We observed a genetically homogeneous population with indications of extensive gene flow. We tentatively attribute this finding to the dispersal potential of the species’ pelagic larvae, supported by marine currents acting as major dispersal vectors. We also detected a signal of expansion towards the poles, consistent with the current climate change scenario. Despite the marked latitudinal differences in the phenotype of reproducing worm pipefish, the absence of clear population structuring suggests that phenotypic plasticity can have a significant role in the expression of sexual selection-related traits.

ACS Style

Tito Mendes; Cidália Gomes; Nuno Monteiro; Agostinho Antunes. Strong Sexual Selection Does Not Induce Population Differentiation in a Fish Species with High Dispersal Potential: The Curious Case of the Worm Pipefish Nerophis lumbriciformis (Teleostei: Syngnathidae). Journal of Urban Health 2020, 111, 585 -592.

AMA Style

Tito Mendes, Cidália Gomes, Nuno Monteiro, Agostinho Antunes. Strong Sexual Selection Does Not Induce Population Differentiation in a Fish Species with High Dispersal Potential: The Curious Case of the Worm Pipefish Nerophis lumbriciformis (Teleostei: Syngnathidae). Journal of Urban Health. 2020; 111 (7):585-592.

Chicago/Turabian Style

Tito Mendes; Cidália Gomes; Nuno Monteiro; Agostinho Antunes. 2020. "Strong Sexual Selection Does Not Induce Population Differentiation in a Fish Species with High Dispersal Potential: The Curious Case of the Worm Pipefish Nerophis lumbriciformis (Teleostei: Syngnathidae)." Journal of Urban Health 111, no. 7: 585-592.

Journal article
Published: 01 September 2020 in G3 Genes|Genomes|Genetics
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The octocoral, Paramuricea clavata, is a habitat-forming anthozoan with a key ecological role in rocky benthic and biodiversity-rich communities in the Mediterranean and Eastern Atlantic. Shallow populations of P. clavata in the North-Western Mediterranean are severely affected by warming-induced mass mortality events (MMEs). These MMEs have differentially impacted individuals and populations of P. clavata (i.e., varied levels of tissue necrosis and mortality rates) over thousands of kilometers of coastal areas. The eco-evolutionary processes, including genetic factors, contributing to these differential responses remain to be characterized. Here, we sequenced a P. clavata individual with short and long read technologies, producing 169.98 Gb of Illumina paired-end and 3.55 Gb of Oxford Nanopore Technologies (ONT) reads. We obtained a de novo genome assembly accounting for 607 Mb in 64,145 scaffolds. The contig and scaffold N50s are 19.15 Kb and 23.92 Kb, respectively. Despite of the low contiguity of the assembly, its gene completeness is relatively high, including 75.8% complete and 9.4% fragmented genes out of the 978 metazoan genes contained in the metazoa_odb9 database. A total of 62,652 protein-coding genes have been annotated. This assembly is one of the few octocoral genomes currently available. This is undoubtedly a valuable resource for characterizing the genetic bases of the differential responses to thermal stress and for the identification of thermo-resistant individuals and populations. Overall, having the genome of P. clavata will facilitate studies of various aspects of its evolutionary ecology and elaboration of effective conservation plans such as active restoration to overcome the threats of global change.

ACS Style

Jean-Baptiste Ledoux; Fernando Cruz; Jèssica Gómez-Garrido; Regina Antoni; Julie Blanc; Daniel Gómez-Gras; Silvija Kipson; Paula López-Sendino; Agostinho Antunes; Cristina Linares; Marta Gut; Tyler S. Alioto; Joaquim Garrabou. The Genome Sequence of the Octocoral Paramuricea clavata – A Key Resource To Study the Impact of Climate Change in the Mediterranean. G3 Genes|Genomes|Genetics 2020, 10, 2941 -2952.

AMA Style

Jean-Baptiste Ledoux, Fernando Cruz, Jèssica Gómez-Garrido, Regina Antoni, Julie Blanc, Daniel Gómez-Gras, Silvija Kipson, Paula López-Sendino, Agostinho Antunes, Cristina Linares, Marta Gut, Tyler S. Alioto, Joaquim Garrabou. The Genome Sequence of the Octocoral Paramuricea clavata – A Key Resource To Study the Impact of Climate Change in the Mediterranean. G3 Genes|Genomes|Genetics. 2020; 10 (9):2941-2952.

Chicago/Turabian Style

Jean-Baptiste Ledoux; Fernando Cruz; Jèssica Gómez-Garrido; Regina Antoni; Julie Blanc; Daniel Gómez-Gras; Silvija Kipson; Paula López-Sendino; Agostinho Antunes; Cristina Linares; Marta Gut; Tyler S. Alioto; Joaquim Garrabou. 2020. "The Genome Sequence of the Octocoral Paramuricea clavata – A Key Resource To Study the Impact of Climate Change in the Mediterranean." G3 Genes|Genomes|Genetics 10, no. 9: 2941-2952.

Journal article
Published: 18 August 2020 in Genomics
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Lung cancer is the type of cancer causing most deaths in humans, with 234,030 new cases of lung cancer diagnosed in the United States in 2018. Recently, Tumor suppressor genes (TSGs) or the control of its pathway became promising drug targets for cancer therapy. A diverse group of TSGs is involved in progression and metastasis of lung cancer. Here, we surveyed nine highly significant mutated genes in 20 mammalian genomes to assess signatures of adaptive evolution using maximum likelihood approaches. We found that three genes (APC, RB1, and TP53) are under strong positive selection, influencing amino acids located in functionally important protein domains, such as three sites in APC found in the APC_N_CC domain, which is responsible for the binding to beta-catenin armadillo repeats that regulate beta-catenin level (beta-catenin is a transcription factor and its misregulation lead to malignant transformation of normal cells). Such sites substitutions mostly increase the stability of the domain. Moreover, substitution of some other sites found in important motifs, such as codon 47 (proline-directed kinase motif) in TP53, modify the phosphorylation activity of TP53 playing a key role in cancer risk. Our findings will open recommendation to drug targeting sites and will foster further research to understand better these proteins function.

ACS Style

Mohamed Emam; João Paulo Machado; Agostinho Antunes. Evolutionary genomics of mammalian lung cancer genes reveals signatures of positive selection in APC, RB1 and TP53. Genomics 2020, 112, 4722 -4731.

AMA Style

Mohamed Emam, João Paulo Machado, Agostinho Antunes. Evolutionary genomics of mammalian lung cancer genes reveals signatures of positive selection in APC, RB1 and TP53. Genomics. 2020; 112 (6):4722-4731.

Chicago/Turabian Style

Mohamed Emam; João Paulo Machado; Agostinho Antunes. 2020. "Evolutionary genomics of mammalian lung cancer genes reveals signatures of positive selection in APC, RB1 and TP53." Genomics 112, no. 6: 4722-4731.

Journal article
Published: 13 July 2020 in Marine Drugs
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Ascidians are marine invertebrates associated with diverse microbial communities, embedded in their tunic, conferring special ecological and biotechnological relevance to these model organisms used in evolutionary and developmental studies. Next-generation sequencing tools have increased the knowledge of ascidians’ associated organisms and their products, but proteomic studies are still scarce. Hence, we explored the tunic of three ascidian species using a shotgun proteomics approach. Proteins extracted from the tunic of Ciona sp., Molgula sp., and Microcosmus sp. were processed using a nano LC-MS/MS system (Ultimate 3000 liquid chromatography system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer). Raw data was searched against UniProtKB – the Universal Protein Resource Knowledgebase (Bacteria and Metazoa section) using Proteome Discoverer software. The resulting proteins were merged with a non-redundant Antimicrobial Peptides (AMPs) database and analysed with MaxQuant freeware. Overall, 337 metazoan and 106 bacterial proteins were identified being mainly involved in basal metabolism, cytoskeletal and catalytic functions. 37 AMPs were identified, most of them attributed to eukaryotic origin apart from bacteriocins. These results and the presence of “Biosynthesis of antibiotics” as one of the most highlighted pathways revealed the tunic as a very active tissue in terms of bioactive compounds production, giving insights on the interactions between host and associated organisms. Although the present work constitutes an exploratory study, the approach employed revealed high potential for high-throughput characterization and biodiscovery of the ascidians’ tunic and its microbiome.

ACS Style

Ana Matos; Dany Domínguez-Pérez; Daniela Almeida; Guillermin Agüero-Chapin; Alexandre Campos; Hugo Osório; Vitor Vasconcelos; Agostinho Antunes. Shotgun Proteomics of Ascidians Tunic Gives New Insights on Host–Microbe Interactions by Revealing Diverse Antimicrobial Peptides. Marine Drugs 2020, 18, 362 .

AMA Style

Ana Matos, Dany Domínguez-Pérez, Daniela Almeida, Guillermin Agüero-Chapin, Alexandre Campos, Hugo Osório, Vitor Vasconcelos, Agostinho Antunes. Shotgun Proteomics of Ascidians Tunic Gives New Insights on Host–Microbe Interactions by Revealing Diverse Antimicrobial Peptides. Marine Drugs. 2020; 18 (7):362.

Chicago/Turabian Style

Ana Matos; Dany Domínguez-Pérez; Daniela Almeida; Guillermin Agüero-Chapin; Alexandre Campos; Hugo Osório; Vitor Vasconcelos; Agostinho Antunes. 2020. "Shotgun Proteomics of Ascidians Tunic Gives New Insights on Host–Microbe Interactions by Revealing Diverse Antimicrobial Peptides." Marine Drugs 18, no. 7: 362.

Journal article
Published: 07 July 2020 in Genes
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Horizontal gene transfer (HGT) plays an important role for evolutionary innovations within prokaryotic communities and is a crucial event for their survival. Several computational approaches have arisen to identify HGT events in recipient genomes. However, this has been proven to be a complex task due to the generation of a great number of false positives and the prediction disagreement among the existing methods. Phylogenetic reconstruction methods turned out to be the most reliable ones, but they are not extensible to all genes/species and are computationally demanding when dealing with large datasets. In contrast, the so-called surrogate methods that use heuristic solutions either based on nucleotide composition patterns or phyletic distribution of BLAST hits can be applied easily to the genomic scale, but they fail in identifying common HGT events. Here, we present ShadowCaster, a hybrid approach that sequentially combines nucleotide composition-based predictions by support vector machines (SVMs) under the shadow of phylogenetic models independent of tree reconstruction, to improve the detection of HGT events in prokaryotes. ShadowCaster successfully predicted close and distant HGT events in both artificial and bacterial genomes. ShadowCaster detected HGT related to heavy metal resistance in the genome of Rhodanobacter denitrificans with higher accuracy than the most popular state-of-the-art computational approaches, encompassing most of the predicted cases made by other methods. ShadowCaster is released at the GitHub platform as an open-source software under the GPLv3 license.

ACS Style

Daniela Sánchez-Soto; Guillermin Agüero-Chapin; Vinicio Armijos-Jaramillo; Yunierkis Perez-Castillo; Eduardo Tejera; Agostinho Antunes; Aminael Sánchez-Rodríguez. ShadowCaster: Compositional Methods under the Shadow of Phylogenetic Models to Detect Horizontal Gene Transfers in Prokaryotes. Genes 2020, 11, 756 .

AMA Style

Daniela Sánchez-Soto, Guillermin Agüero-Chapin, Vinicio Armijos-Jaramillo, Yunierkis Perez-Castillo, Eduardo Tejera, Agostinho Antunes, Aminael Sánchez-Rodríguez. ShadowCaster: Compositional Methods under the Shadow of Phylogenetic Models to Detect Horizontal Gene Transfers in Prokaryotes. Genes. 2020; 11 (7):756.

Chicago/Turabian Style

Daniela Sánchez-Soto; Guillermin Agüero-Chapin; Vinicio Armijos-Jaramillo; Yunierkis Perez-Castillo; Eduardo Tejera; Agostinho Antunes; Aminael Sánchez-Rodríguez. 2020. "ShadowCaster: Compositional Methods under the Shadow of Phylogenetic Models to Detect Horizontal Gene Transfers in Prokaryotes." Genes 11, no. 7: 756.

Journal article
Published: 04 May 2020 in Proceedings of the National Academy of Sciences
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Lions are one of the world’s most iconic megafauna, yet little is known about their temporal and spatial demographic history and population differentiation. We analyzed a genomic dataset of 20 specimens: two ca. 30,000-y-old cave lions (Panthera leo spelaea), 12 historic lions (Panthera leo leo/Panthera leo melanochaita) that lived between the 15th and 20th centuries outside the current geographic distribution of lions, and 6 present-day lions from Africa and India. We found that cave and modern lions shared an ancestor ca. 500,000 y ago and that the 2 lineages likely did not hybridize following their divergence. Within modern lions, we found 2 main lineages that diverged ca. 70,000 y ago, with clear evidence of subsequent gene flow. Our data also reveal a nearly complete absence of genetic diversity within Indian lions, probably due to well-documented extremely low effective population sizes in the recent past. Our results contribute toward the understanding of the evolutionary history of lions and complement conservation efforts to protect the diversity of this vulnerable species.

ACS Style

Marc de Manuel; Ross Barnett; Marcela Sandoval-Velasco; Nobuyuki Yamaguchi; Filipe Garrett Vieira; Marie Lisandra Zepeda Mendoza; Shiping Liu; Michael D. Martin; Mikkel-Holger S. Sinding; Sarah S. T. Mak; Christian Carøe; Shanlin Liu; Chunxue Guo; Jiao Zheng; Grant Zazula; Gennady Baryshnikov; Eduardo Eizirik; Klaus-Peter Koepfli; Warren E. Johnson; Agostinho Antunes; Thomas Sicheritz-Ponten; Shyam Gopalakrishnan; Greger Larson; Huanming Yang; Stephen J. O’Brien; Anders J. Hansen; Guojie Zhang; Tomas Marques-Bonet; M. Thomas P. Gilbert. The evolutionary history of extinct and living lions. Proceedings of the National Academy of Sciences 2020, 117, 10927 -10934.

AMA Style

Marc de Manuel, Ross Barnett, Marcela Sandoval-Velasco, Nobuyuki Yamaguchi, Filipe Garrett Vieira, Marie Lisandra Zepeda Mendoza, Shiping Liu, Michael D. Martin, Mikkel-Holger S. Sinding, Sarah S. T. Mak, Christian Carøe, Shanlin Liu, Chunxue Guo, Jiao Zheng, Grant Zazula, Gennady Baryshnikov, Eduardo Eizirik, Klaus-Peter Koepfli, Warren E. Johnson, Agostinho Antunes, Thomas Sicheritz-Ponten, Shyam Gopalakrishnan, Greger Larson, Huanming Yang, Stephen J. O’Brien, Anders J. Hansen, Guojie Zhang, Tomas Marques-Bonet, M. Thomas P. Gilbert. The evolutionary history of extinct and living lions. Proceedings of the National Academy of Sciences. 2020; 117 (20):10927-10934.

Chicago/Turabian Style

Marc de Manuel; Ross Barnett; Marcela Sandoval-Velasco; Nobuyuki Yamaguchi; Filipe Garrett Vieira; Marie Lisandra Zepeda Mendoza; Shiping Liu; Michael D. Martin; Mikkel-Holger S. Sinding; Sarah S. T. Mak; Christian Carøe; Shanlin Liu; Chunxue Guo; Jiao Zheng; Grant Zazula; Gennady Baryshnikov; Eduardo Eizirik; Klaus-Peter Koepfli; Warren E. Johnson; Agostinho Antunes; Thomas Sicheritz-Ponten; Shyam Gopalakrishnan; Greger Larson; Huanming Yang; Stephen J. O’Brien; Anders J. Hansen; Guojie Zhang; Tomas Marques-Bonet; M. Thomas P. Gilbert. 2020. "The evolutionary history of extinct and living lions." Proceedings of the National Academy of Sciences 117, no. 20: 10927-10934.

Journal article
Published: 01 May 2020 in Regional Studies in Marine Science
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Studying the diet of crustaceans based on the direct visual inspection of the gut contents can be difficult because they can ingest only a portion of the prey and further crush it into very small pieces, making a very few food items identifiable. Here, the usefulness of molecular methods to identify the species present in the gut contents of two crustaceans, the shore crab Carcinus maenas and the brown shrimp Crangon crangon, was tested. Stomach contents were removed, and their total genomic DNA extracted followed by amplification (PCR) with four specific prey mtDNA primers. Since both crustaceans are opportunistic predators, preying upon around 40 different species, the study was restricted to common potential preys in the sampled area, the Minho Estuary (Portugal): the common goby Pomatoschistus microps, the ragworm Hediste diversicolor, the flounder Platichthys flesus and the peppery furrow shell Scrobicularia plana. After testing the most appropriate methodology for DNA extraction and PCR amplification to detect the presence of each potential prey with positive control samples, the same methodology was applied to the stomach content samples. The molecular methods allowed for detection of the presence of P. microps, P. flesus and H. diversicolor in the stomachs of both crustaceans. Therefore, once potential prey and their availability in the system are known, PCR technique can be an alternative tool to overcome some of the limitations of traditional methods in the study of the crustaceans’ diet, even when stomach contents include a great part of ‘detritus’. This study reflects also the need to develop more tools (primers) to improve the assessment of predator–prey interactions in aquatic ecosytems.

ACS Style

Joana Campos; Cristiana Moreira; Sérgia Costa-Dias; Eunice Ferreira; Ana Matos; Vítor Vasconcelos; Agostinho Antunes. Polymerase chain reaction as a promising tool for DNA-based diet studies of crustaceans. Regional Studies in Marine Science 2020, 37, 101340 .

AMA Style

Joana Campos, Cristiana Moreira, Sérgia Costa-Dias, Eunice Ferreira, Ana Matos, Vítor Vasconcelos, Agostinho Antunes. Polymerase chain reaction as a promising tool for DNA-based diet studies of crustaceans. Regional Studies in Marine Science. 2020; 37 ():101340.

Chicago/Turabian Style

Joana Campos; Cristiana Moreira; Sérgia Costa-Dias; Eunice Ferreira; Ana Matos; Vítor Vasconcelos; Agostinho Antunes. 2020. "Polymerase chain reaction as a promising tool for DNA-based diet studies of crustaceans." Regional Studies in Marine Science 37, no. : 101340.

Research article
Published: 14 April 2020 in Aquatic Conservation: Marine and Freshwater Ecosystems
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Understanding the interactions among demographic parameters, mating system and population dynamics is key to predict the response of populations to global change. The Mediterranean red coral is a precious octocoral suffering from population decline due to overfishing and warming‐driven mass mortality events. While the demographic consequences of these two pressures are well characterized, little is known regarding their impact on population dynamics and evolution of red coral populations. The main objective of this study was to fill this gap focusing more particularly on mating pattern and genetic drift. Combining sibship and progeny arrays analyses, a genetic characterization of the red coral mating system was conducted. In addition, a synchronic approach was developed comparing mating patterns in two populations with contrasting demographic patterns: a pristine‐like population and a declining population. The results show that polyandry is likely to be the norm in red coral. The similar patterns of genetic diversity between adults and larvae combined with the lack of differential reproductive success among putative fathers did not support significant sweepstakes effects during larval production. While instantaneous biparental inbreeding was detected, no long‐term inbreeding was observed even in the declining population. Mating patterns and effective population sizes in the two populations were not statistically different. Nevertheless, a trend towards a slightly higher inbreeding and a lower number of breeders was observed in the declining population. Accordingly, we hypothesized that an increase in male gamete dispersal may buffer the increase of genetic drift expected in the declining population. This feedback between demographic decline and reproductive pattern may potentially take part in the long‐term persistence of red coral populations. However, the negative trend reported in the declining population unambiguously supports the need to maintain high densities of reproductive colonies to the functioning of red coral populations.

ACS Style

Jean‐Baptiste Ledoux; Silvia Frias‐Vidal; Ignasi Montero‐Serra; Agostinho Antunes; Clara Casado Bueno; Sergi Civit; Paula Lopez‐Sendino; Cristina Linares; Joaquim Garrabou. Assessing the impact of population decline on mating system in the overexploited Mediterranean red coral. Aquatic Conservation: Marine and Freshwater Ecosystems 2020, 30, 1149 -1159.

AMA Style

Jean‐Baptiste Ledoux, Silvia Frias‐Vidal, Ignasi Montero‐Serra, Agostinho Antunes, Clara Casado Bueno, Sergi Civit, Paula Lopez‐Sendino, Cristina Linares, Joaquim Garrabou. Assessing the impact of population decline on mating system in the overexploited Mediterranean red coral. Aquatic Conservation: Marine and Freshwater Ecosystems. 2020; 30 (6):1149-1159.

Chicago/Turabian Style

Jean‐Baptiste Ledoux; Silvia Frias‐Vidal; Ignasi Montero‐Serra; Agostinho Antunes; Clara Casado Bueno; Sergi Civit; Paula Lopez‐Sendino; Cristina Linares; Joaquim Garrabou. 2020. "Assessing the impact of population decline on mating system in the overexploited Mediterranean red coral." Aquatic Conservation: Marine and Freshwater Ecosystems 30, no. 6: 1149-1159.

Journal article
Published: 05 April 2020 in International Journal of Molecular Sciences
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Adhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental composites. This study was focused on the goose barnacle Pollicipes pollicipes adhesion system, a species that lives in the Eastern Atlantic strongly exposed intertidal rocky shores and cliffs. The protein composition of P. pollicipes cement multicomplex and cement gland was quantitatively studied using a label-free LC-MS high-throughput proteomic analysis, searched against a custom transcriptome-derived database. Overall, 11,755 peptide sequences were identified in the gland while 2880 peptide sequences were detected in the cement, clustered in 1616 and 1568 protein groups, respectively. The gland proteome was dominated by proteins of the muscle, cytoskeleton, and some uncharacterized proteins, while the cement was, for the first time, reported to be composed by nearly 50% of proteins that are not canonical cement proteins, mainly unannotated proteins, chemical cues, and protease inhibitors, among others. Bulk adhesive proteins accounted for one-third of the cement proteome, with CP52k being the most abundant. Some unannotated proteins highly expressed in the proteomes, as well as at the transcriptomic level, showed similar physicochemical properties to the known surface-coupling barnacle adhesive proteins while the function of the others remains to be discovered. New quantitative and qualitative clues are provided to understand the diversity and function of proteins in the cement of stalked barnacles, contributing to the whole adhesion model in Cirripedia.

ACS Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André M. Machado; Lothar Jänsch; Luís Filipe Castro; Agostinho Antunes; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes. International Journal of Molecular Sciences 2020, 21, 2524 .

AMA Style

Dany Domínguez-Pérez, Daniela Almeida, Josef Wissing, André M. Machado, Lothar Jänsch, Luís Filipe Castro, Agostinho Antunes, Vitor Vasconcelos, Alexandre Campos, Isabel Cunha. The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes. International Journal of Molecular Sciences. 2020; 21 (7):2524.

Chicago/Turabian Style

Dany Domínguez-Pérez; Daniela Almeida; Josef Wissing; André M. Machado; Lothar Jänsch; Luís Filipe Castro; Agostinho Antunes; Vitor Vasconcelos; Alexandre Campos; Isabel Cunha. 2020. "The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, Pollicipes pollicipes." International Journal of Molecular Sciences 21, no. 7: 2524.