Soybean is one of the world’s most consumed crops. As the human population continuously increases, new phenotyping technology is needed to develop new soybean varieties with high-yield, stress-tolerant, and disease-tolerant traits. Hyperspectral imaging (HSI) is one of the most used technologies for phenotyping. The current HSI techniques with indoor imaging towers and unmanned aerial vehicles (UAVs) suffer from multiple major noise sources, such as changes in ambient lighting conditions, leaf slopes, and environmental conditions. To reduce the noise, a portable single-leaf high-resolution HSI imager named LeafSpec was developed. However, the original design does not work efficiently for the size and shape of dicot leaves, such as soybean leaves. In addition, there is a potential to make the dicot leaf scanning much faster and easier by automating the manual scan effort in the original design. Therefore, a renovated design of a LeafSpec with increased efficiency and imaging quality for dicot leaves is presented in this paper. The new design collects an image of a dicot leaf within 20 s. The data quality of this new device is validated by detecting the effect of nitrogen treatment on soybean plants. The improved spatial resolution allows users to utilize the Normalized Difference Vegetative Index (NDVI) spatial distribution heatmap of the entire leaf to predict the nitrogen content of a soybean plant. This preliminary NDVI distribution analysis result shows a strong correlation (R² = 0.871) between the image collected by the device and the nitrogen content measured by a commercial laboratory. Therefore, it is concluded that the new LeafSpec-Dicot device can provide high-quality hyperspectral leaf images with high spatial resolution, high spectral resolution, and increased throughput for more accurate phenotyping. This enables phenotyping researchers to develop novel HSI image processing algorithms to utilize both spatial and spectral information to reveal more signals in soybean leaf images.

Weed control is fundamental to modern agriculture, underpinning crop productivity, food security, and the economic sustainability of farming operations. Herbicides have long been the cornerstone of effective weed management, significantly enhancing agricultural yields over recent decades. However, the field now faces critical challenges, including stagnation in the discovery of new herbicide modes of action (MOAs) and the escalating prevalence of herbicide-resistant weed populations. High research and development costs, coupled with stringent regulatory hurdles, have impeded the introduction of novel herbicides, while the widespread reliance on glyphosate-based systems has accelerated resistance development. In response to these issues, advanced image-based plant phenotyping technologies have emerged as pivotal tools in addressing herbicide-related challenges in weed science. Utilizing sensor technologies such as hyperspectral, multispectral, RGB, fluorescence, and thermal imaging methods, plant phenotyping enables the precise monitoring of herbicide drift, analysis of resistance mechanisms, and development of new herbicides with innovative MOAs. The integration of machine learning algorithms with imaging data further enhances the ability to detect subtle phenotypic changes, predict herbicide resistance, and facilitate timely interventions. This review comprehensively examines the application of image phenotyping technologies in weed science, detailing various sensor types and deployment platforms, exploring modeling methods, and highlighting unique findings and innovative applications. Additionally, it addresses current limitations and proposes future research directions, emphasizing the significant contributions of phenotyping advancements to sustainable and effective weed management strategies. By leveraging these sophisticated technologies, the agricultural sector can overcome existing herbicide challenges, ensuring continued productivity and resilience in the face of evolving weed pressures.

Image-based spectroscopy phenotyping is a rapidly growing field that investigates how genotype, environment and management interact using remote or proximal sensing systems to capture images of a plant under multiple wavelengths of light. While remote sensing techniques have proven effective in crop phenotyping, they can be subject to various noise sources, such as varying lighting conditions and plant physiological status, including leaf orientation. Moreover, current proximal leaf-scale imaging devices require the sensors to accommodate the state of the samples during imaging which induced extra time and labor cost. Therefore, this study developed a proximal multispectral imaging device that can actively attract the leaf to the sensing area (target-to-sensor mode) for high-precision and high-throughput leaf-scale phenotyping. To increase the throughput and to optimize imaging results, this device innovatively uses active airflow to reposition and flatten the soybean leaf. This novel mechanism redefines the traditional sensor-to-target mode and has relieved the device operator from the labor of capturing and holding the leaf, resulting in a five-fold increase in imaging speed compared to conventional proximal whole leaf imaging device. Besides, this device uses artificial lights to create stable and consistent lighting conditions to further improve the quality of the images. Furthermore, the touch-based imaging device takes full advantage of proximal sensing by providing ultra-high spatial resolution and quality of each pixel by blocking the noises induced by ambient lighting variances. The images captured by this device have been tested in the field and proven effective. Specifically, it has successfully identified nitrogen deficiency treatment at an earlier stage than a typical remote sensing system. The p-value of the data collected by the device (p = 0.008) is significantly lower than that of a remote sensing system (p = 0.239).

Spectral imaging has been widely applied in plant phenotyping to assess corn leaf nitrogen status. Recent studies indicate that spatial variations within a single leaf’s multispectral image provide stronger signals for corn nitrogen estimation. However, current technologies for corn multispectral imaging cannot capture a large corn leaf segment with high-resolution and simple operation, limiting their efficiency and accuracy in nitrogen estimation. To address this gap, this study developed a proximal multispectral imaging device that can capture high-resolution snapshot multispectral images of a large segment of a single corn leaf. This device uses airflow to autonomously position and flatten the leaf to minimize the noise in images due to leaf curvature and simplify operation. Moreover, this device adopts a transmittance imaging regime by clamping the corn leaf between the camera and the lighting source to block the environmental lights and supply uniform lighting to capture high-resolution and high-precision leaf images within six seconds. A field assay was conducted to validate the effectiveness of the multispectral images captured by this device in assessing nitrogen status by classifying the nitrogen treatments applied to corn. Six nitrogen treatments were applied to 12 plots of corn fields, and 10 images were collected at each plot. By using the average vegetative index of the whole image, only one treatment was significantly different from the other five treatments, and no significant difference was observed among any other groups. However, by extracting the spatial and spectral features from the images and combining these features, the accuracy of nitrogen treatment classification improved compared to using the average index. In another analysis, by applying spatial–spectral analysis methods to the images, the nitrogen treatment classification accuracy has improved compared to using the average index. These results demonstrated the advantages of this high-resolution and high-throughput imaging device for distinguishing nitrogen treatments by facilitating spatial–spectral combined analysis for more precise classification.

Nitrogen (N) deficiency detection during early vegetative stages is essential for guiding timely fertilization and sustaining maize productivity. Spatial-spectral features derived from hyperspectral imagery have been widely used to diagnose maize N status. However, features derived from single time points provide only static representations of plant responses and may fail to capture the subtle physiological dynamics during the early stages of N stress. In this study, a temporal spatial–spectral (TSS) feature framework based on hyperspectral transmittance imaging was proposed to detect maize N status during early vegetative stages. Results indicated that neither leaf chlorophyll content index nor averaged spectral transmittance curves provided clear separability prior to 8 days after treatment (DAT). In contrast, the random forest models built on TSS features successfully identified the maize under LN treatment using temporal variations captured between 2 and 4 DAT. The model exhibited robust discriminative capacity achieving a testing accuracy of 87.50%. An ensemble learning strategy that integrated predictions from the top-performing TSS-based models further improved the testing accuracy to 89.50%. Visualization of the most informative TSS features revealed pronounced dynamic differences between HN and LN groups. The TSS-based heatmaps effectively captured the symptom of early N stress progression that remained undetectable in pseudo RGB images or the normalized difference vegetation index (NDVI) heatmaps. Overall, the proposed TSS framework provides an effective and interpretable approach for early nitrogen status detection and offers a promising foundation for precision crop nitrogen management.