Breast cancer susceptibility gene 2 (BRCA2) is the main gene associated with hereditary breast cancers. However, a mutation in BRCA2 has also been found in other tumors, such as ovarian, pancreatic, thyroid, gastric, laryngeal, and prostate cancers. In this review, we discuss the biological functions of BRCA2 and the role of BRCA2 mutations in tumor progression and therapy.

Background: Day surgery has become the main mode of surgery in American and European countries, but it is still in the early stage in developing countries due to the limitation of medical technology and the backward management concept. At present, day surgery accounts for more than 60% of elective surgery in many countries in Europe and North America and more than 85% in countries such as the United Kingdom and the United States. There are 8469 ambulatory surgery centers in the United States in 2023. In China, the first ambulatory surgery center was established in 2001. In 2018, more than half of the tertiary hospitals (high‐level hospitals) in China carried out day surgery, of which 639 hospitals set up ambulatory surgery centers; the proportion of day surgery in elective surgery increased to 12.8%. The annual number of day surgeries exceeded 1.25 million. In 2022, our hospital established an ambulatory surgery center managed by anesthesiologists. Day surgery requires anesthesiologists to participate in the whole process of patient management from preoperative preparation to postoperative recovery. The establishment of ambulatory surgery centers managed by anesthesiologists is of great significance to China, developing countries, and the whole world.Objectives: So this study aimed to review the development of day surgery in China, combine Chinese government policy evolution, summarize the management model of Chinese ambulatory surgery centers, guide the establishment of ambulatory surgery centers in low‐ and middle‐income countries, and highlight and analyze the advantages of anesthesiologist‐managed ambulatory surgery centers as distinct from other physician‐managed ones.Discussion: We call on anesthesiologists, other physicians, surgeons, nurses, and health system managers around the world to promote efficient, low‐cost day surgery in developing countries and thereby increase access to surgical treatment for the world's poor.

Background: Human papillomavirus (HPV) screening and vaccination exert efficacy in controlling the progression of cervical cancer. Thus, examinations into HPV prevalence, age‐stratified specificity, genotype distribution, and their correlation with pathological outcomes can furnish robust evidence for customizing high‐quality population screening and management.Methods: A cohort of 17,923 women attending clinics in the Jintang area, Sichuan, from January 2019 through August 2023 were enrolled in the study. Genotyping of HPV was conducted using real‐time polymerase chain reaction (RT‐PCR). The epidemiology and the relationship between HPV infection and histologic/cytologic abnormalities were subjected to analysis.Results: HPV infection was identified in 4387 women. The outpatient group exhibited a significantly higher HPV infection rate compared to the healthy examination group (26.5% vs. 17.5%, p < 0.05). The distribution of infection rates across different age groups exhibited a U‐shaped pattern, with the highest infection rate in the group ≤20 years of age, succeeded by those >60 years of age. The 31–40 age group demonstrated the lowest prevalence of infection, but upon infection, its prevalence of the precancerous lesion CIN2‐3 reached a maximum of 29.0%, constituting a novel finding. The most prevalent genotype was HPV52, followed by HPV16, 58, 53, 68, and 18. In the cytologic and histologic abnormalities group, the most common types were HPV52, 16, and 58. HPV16 predominantly appeared in high‐grade intraepithelial neoplasia and carcinoma in situ, constituting over 60% of cases. While HPV type 52 was not individually detected in cervical cancer cases. And some other non‐vaccine‐covered HPV subtypes also showed high prevalence in Sichuan. The single infection rates of NH9‐HPV (high‐risk HPV subtypes covered by the non‐nine‐valent vaccine) in CIN2‐3 and cervical cancer patients were 6.5% and 2.6%, respectively. Among them, HPV51, HPV53, HPV59, and HPV35 exhibited a significant preponderance, which even higher than HPV45 and HPV31 covered by the nine‐valent vaccine types. And in NL9‐HPV (low‐risk HPV subtypes covered by the non‐nine‐valent vaccine), HPV42 accounted for the highest percentage in CIN2‐3. A similar decreasing trend was observed in annual infection rates in the healthy examination population and in the 31–40 and 51–60 age groups, while the ≤20 age group showed an increase. Regarding type‐specificity, HPV16 and HPV58 exhibited the most rapid declines.Conclusion: This study furnishes the latest insights into the characteristics of HPV infection rate, age distribution, and genotype prevalence in Sichuan.

Multiple myeloma (MM), the second most common hematologic malignancy, is characterized by the clonal expansion of myeloma cells and accumulation of genetic lesions. MM progression is accompanied by increased aggressiveness and drug resistance. Even the goal of “cure” remains hard to reach for most patients, advances in diagnosis and treatment have allowed some to achieve durable remissions and transition to plateau phase. Single-cell sequencing, with its powerful ability to analyze cellular heterogeneity and molecular patterns at ground-breaking resolution, is informative for deciphering tumors and their microenvironment. In this review, we summarize the new insights of studies facilitated by emerging single-cell sequencing into clonal evolution, myeloma-supported microenvironment transformation, epigenetic changes, and novel prognostic and therapeutic strategies for MM, revealing the key mechanisms underlying MM progression and the direction of future efforts. With the continuous expansion of the research scope and optimization of related technologies, single-cell sequencing is expected to revolutionize our understanding of the biology and evolutionary dynamics of MM and contribute to the radical and precise improvement of treatment.

Purpose: To innovatively use the FOCUS‐PDCA quality improvement strategy to establish an external quality assessment (EQA) working group to continuously improve EQA performance, an important indicator of the national tertiary public hospital performance appraisal. Methods: The project was carried out at the National Center for Clinical Laboratories. Using FOCUS‐PDCA, which combines problem‐focused steps (FOCUS) and improvement steps (PDCA), a project team was established to carry out improvement work. Root cause analysis was carried out to analyze the problems in quality control from EQA project application to results analysis and an improvement plan was implemented according to the steps of FOCUS‐PDCA. The project was executed in three cycles from 2019 to 2021 to obtain more satisfactory results. Results: After implementing three cycles of FOCUS‐PDCA, the EQA participation rate increased from 66.5% in 2018 to 100% in 2021, and the EQA pass rate increased from 94.9% in 2018 to 99.3% in 2021. Consequently, the hospital moved into the top 50 in performance assessment for the first time in 2020 and ranked 27th in 2021. Conclusion: The use of the FOCUS‐PDCA quality improvement strategy can improve the EQA performance of national tertiary public hospitals and help them achieve satisfactory results in the national examination.