Stenotrophomonas maltophilia is a multidrug-resistant bacterium with no precise clinical treatment. This bacterium can be a vital cause for death and different organ failures in immune-compromised, immune-competent, and long-time hospitalized patients. Extensive quorum sensing capability has become a challenge to develop new drugs against this pathogen. Moreover, the organism possesses about 789 proteins which function, structure, and pathogenesis remain obscured. In this piece of work, we tried to enlighten the aforementioned sectors using highly reliable bioinformatics tools validated by the scientific community. At first, the whole proteome sequence of the organism was retrieved and stored. Then we separated the hypothetical proteins and searched for the conserved domain with a high confidence level and multi-server validation, which resulted in 24 such proteins. Furthermore, all of their physical and chemical characterizations were performed, such as theoretical isoelectric point, molecular weight, GRAVY value, and many more. Besides, the subcellular localization, protein-protein interactions, functional motifs, 3D structures, antigenicity, and virulence factors were also evaluated. As an extension of this work, 'RTFAMSSER' and 'PAAPQPSAS' were predicted as potential T and B cell epitopes, respectively. We hope our findings will help in better understating the pathogenesis and smoothen the way to the cure.

• Arsenic exposure through food causes genotoxic effects in vivo. • Sub chronic exposure to Arsenic causes pre-cancerous changes. • C. olitorious & B. monosperma have significant nephro & hepato protective potential. • Protective effect of both plants species is evident in molecular level. • Potent use as readily available food supplement to minimize Arsenic toxicity.

Background: Autism Spectrum Disorder (ASD) is a group of neurodevelopmental disorder which is now a hotbed worldwide. Being located on chromosome X and playing a vital role in synaptic transmission, NLGN-3 and 4X have been drawn the attention of many researchers as the most essential and functional candidate genes for ASD pathogenesis. However, there are many contradictory results considering their role in developing ASD in different populations.Objective: This study was aimed to investigate the association of NLGN-3 and 4X genes along with non-genetic factors to develop ASD in Bangladeshi population.Methods: In this study, we analysed rs4844285 and rs11795613 of NLGN-3 gene and rs3810686 and rs6638575 of NLGN-4X gene for both family-based association analysis and case-control based association analysis using polymerase chain reaction (PCR) and DNA sequencing. Along with this, demographic data were also analysed. A total of 60 members of 15 families, including ASD subjects and another 60 ASD subjects and 60 healthy people were included in this study.Results: Allele A and genotype AA of NLGN-3 rs4844285 were found to be a probable risk factor for developing ASD in our studied population (p=0.031 for allele A, S x2 = 2.707, OR=1.833 for genotype AA). We also found different birth complications in this studied population which can be considered to intensify the risk of ASD along with the abnormalities of genes.Conclusion: From this study, it is clear that not only genetic abnormalities but also some birth anomalies play a vital role for the risk of developing ASD. Bangladesh Med Res Counc Bull 2021; 47(3): 250-259