Background: Chronic wounds carry financial burdens and increase morbidity and mortality, especially in diabetic ulcers and Hansen’s Morbus. More than 50% of chronic ulcers are difficult to heal with regular treatment and require new types of therapy such as the use of secretome of human umbilical cord mesenchymal stem cells (SM-hUCMSC). Methods: This experimental study was carried out to see the effectiveness of using SM-hUCMSC in diabetic ulcers and Hansen’s Morbus in four medical facilities (multicentre). The level of active secretion has been measured by default in 10% SM-hUCMSC gel, used as a treatment intervention. The primary outcome is wound healing in terms of the length, width, and extent of the wound. The secondary is the side effects of treatment 2 weeks after administration. Follow-up visits will be scheduled at 1 and 2 weeks post-treatment. Results: Forty-one chronic ulcers successfully followed the study until the end. In patients with chronic ulcers, the mean ulcer length, width, and area were 1.60 (0,50– 13,0), 1.3 (0,5– 6,0), and 2.21 (0,25– 78) cm square, respectively, before interventions and 1 (0– 12), 0,8 (0– 6,0), and 1 (0– 72) square cm after interventions at the second follow-up. The change between the beginning and end of the intervention was significant (p-value < 0.05). Conclusion: The use of 10% SM-hUCMSC gel topically has been proven effective in accelerating the process of wound healing, especially chronic ulcers with side effects that are not present in this study.

Background and Aim: Human umbilical cord mesenchymal stem cells (hUC-MSCs) and its conditioned medium (CM) promote wound healing. This study investigated the wound healing potential of hUC-MSC CM in vitro and in vivo using diabetic animal models. Materials and Methods: The CM from hUC-MSC CM prepared under hypoxic conditions (hypoxic hUC-MSC) was evaluated for stimulating rat fibroblast growth, collagen production (in vitro), and wound healing in animal models (in vivo). An excision wound on the dorsal side of the diabetes-induced rats was established, and the rats were randomly divided into non-treatment, antibiotic, and hypoxic hUC-MSC CM groups. The cell number of fibroblasts and collagen secretion was evaluated and compared among the groups in an in vitro study. By contrast, wound size reduction, width of re-epithelialization, and the collagen formation area were assessed and compared among the groups in an in vivo study. Results: CM under hypoxic conditions contained a higher concentration of wound healing-related growth factors. Hypoxic hUC-MSC CM could facilitate fibroblast cell growth and collagen synthesis, although not significant compared with the control group. Re-epithelialization and collagen production were higher in the hUC-MSC CM group than in the antibiotic and non-treatment groups. Conclusion: Hypoxic hUC-MSC CM possessed more positive effects on the wound healing process based on re-epithelialization and collagen formation than antibiotic treatment did.

Sumber daya alam yang beragam di Indonesia memiliki banyak manfaat terutama dalam mengatasi penyakit, seperti penuaan, kanker, kardiovaskular, gangguan neurologis dan autoimun. Salah satu yang berperan dalam perkembangan penyakit tersebut adalah stres oksidatif dimana terjadi ketidakseimbangan antara produksi serta akumulasi spesies reaktif oksigen (ROS) dengan jumlah antioksidan dalam tubuh yang berperan mendetoksifikasi produk reaktif ini. Antioksidan dapat berasal baik dari endogen maupun eksogen. Dalam upaya menghindari terjadinya stres oksidatif, tubuh memerlukan antioksidan eksogen. Daun salam merupakan salah satu sumber antioksidan eksogen. Beberapa penelitian menyebutkan bahwa daun salam kaya akan antioksidan, maka dari itu, penelitian ini ingin menggali lebih dalam mengenai kandungan antioksidan pada daun salam. Penelitian eksperimental bersifat in vitro ini mencakup uji fitokimia dan uji kapasitas antioksidan. Ekstrak daun salam didapatkan dengan teknik perkolasi menggunakan pelarut metanol. Alat spektrofotometer UV-Vis digunakan untuk mendapatkan data yang kemudian diolah menggunakan GraphPad Prism v.7.0 La Jolla, California, USA. Pada uji fitokimia didapatkan bahwa daun salam mengandung alkaloid, flavonoid, saponin, betasianin, tanin, steroid, terpenoid, fenol, kardio glikosida, dan kuinon. Hasil uji kapasitas total antioksidan dengan metode metode ABTS yakni IC50 46,416 µg/mL, DPPH yakni IC50 168,4 µg/mL, dan metode FRAP IC50 yakni 11,40 µg/mL. Hasil yang didapatkan menunjukkan bahwa potensi sebagai antioksidan yang dimiliki daun salam termasuk golongan kuat.

The human autologous hepatocyte matrix implant is a promising alternative procedure to counter liver damage. We assessed the outcome of human hepatocytes isolation from cirrhotic liver compared to the clinical and histological scores of disease severity. A total of 11 patients with various clinical scores (CTP and MELD) and histological score (Metavir, fibrosis) of liver cirrhosis were included in the hepatocyte matrix implant clinical phase I study. The liver segment and pancreatic tissue were harvested from each patient, and hepatocytes and cells of islets of Langerhans were isolated. The freshly isolated human hepatocytes were coseeded with the islet cells onto poly(l-lactic acid) (PLLA) scaffolds, cultured, and transplanted back into the patient. Human hepatocytes were isolated from 11 cirrhotic liver specimens with a resulting yield of 1.40.510⁶ cells per gram of the liver specimen and a viability rate of 5213. It was found that the yield and viability of the liver cells were not correlated with the clinical and histological scores of the liver cirrhosis. A correlation was found between the hepatocyte yield obtained and the average number of hepatocytes counted in 10 microscopic fields of view. More viable cells were obtained from cirrhotic livers caused by chronic hepatitis B as compared to chronic hepatitis C in the same MELD score range. There was no correlation between the clinical and histological disease severity scores of liver cirrhosis and the outcome of hepatocytes isolation. It seems that the yield could depend on the type of hepatitis underlying the cirrhotic tissue. The study was registered at www.clinicaltrial.gov with the study identifier: NCT01335568.