Biocontrol strategies are gaining tremendous attention in insect pest management, such as controlling termite damage, due to the growing awareness of the irreparable harm caused by the continuous use of synthetic pesticides. This study examines the proteolytic and chitinolytic activities of Bacillus velezensis CE 100 and its termiticidal effect through cuticle degradation. The proteolytic and chitinolytic activities of B. velezensis CE 100 systematically increased with cell growth to the respective peaks of 68.3 and 128.3 units/mL after seven days of inoculation, corresponding with the highest cell growth of 16 × 10⁷ colony-forming units (CFU)/mL. The in vitro termiticidal assay showed that B. velezensis CE 100 caused a rapid and high rate of termite mortality, with a median lethal time (LT50) of >1 h and the highest mortality rates of 91.1% and 92.2% recorded at 11 h and 12 h in the bacterial broth culture and crude enzyme fraction, respectively. In addition to broken setae and deformed sockets, termites treated with the bacterial broth culture exhibited degraded epicuticles, while the crude enzyme fraction caused severe disintegration of both the epicuticle and endocuticle. These results indicate the tremendously higher potential of B. velezensis CE 100 in the biological control of subterranean termites compared to the previously used entomopathogenic bacteria.

Subterranean termites of the species Reticulitermes speratus kyushuensis Morimoto (Isoptera: Rhinotermitidae) are notoriously destructive soil-dwelling pests that feed on the cellulosic wood biomass. This leads to tremendous losses of forest trees such as Pinus densiflora Siebold and Zucc. (Pinales: Pinaceae) and precious wooden structures of cultural heritage. This study investigated the efficacy of chitinase and protease produced by Bacillus licheniformis PR2 as cuticle-degrading enzymes for the biocontrol of worker termites. Bacillus licheniformis PR2 produced a strong chitinase and protease activity up to a maximum of 82.3 unit/mL and 35.9 unit/mL, respectively, and caused a lethal effect on termites under laboratory conditions. Treatment of termites with the bacterial broth culture and the crude enzyme fraction of B. licheniformis PR2 resulted in a maximum mortality rate (with a median lethal time (ET50)) of 83.3% (3 h, 36 min) and 88.9% (2 h, 59 min), respectively. The termites treated with B. licheniformis PR2 exhibited loss of setae, disintegration of epicuticle, rupturing of procuticle, and swelling at sockets. The degradation of cuticular chitin and glycoprotein polymers in the termite cuticle by chitinase and protease enzymes produced by B. licheniformis PR2 represents an effective eco-friendly strategy for controlling termite damage in Korean cultural heritage sites and forests.

This study investigates the antifungal effect of slightly acidic electrolyzed water on Botrytis cinerea, an agent that causes gray mold to appear on the cut rose flower of the type known as 'Bubble Gum'. Petals were inoculated by spraying a spore suspension with 3 × 10 5 spores•mL-1 (1.2 mL per flower) which was then allowed to dry in air for an hour. The petal pulsing treatments used here were as follows: no treatment (NT, control) and HOCl treatments at concentrations of 0, 5, 10, 20, and 30 µL•L-1 sprayed for three seconds. The treatment with 30 µL•L-1 HOCl (27.8%) reduced B. cinerea by 71% compared to NT (94.6%) and by 68% compared to the 0 µL•L-1 HOCl (87.9%). The vase life of cut rose flowers was lowest in the NT and highest with 30 µL•L-1 HOCl. The disease incidence rate according to the visual index of B. cinerea decreased as the HOCl concentration was increased. The petal color did not differ among the treatments in terms of the L*, a*, and b* values, except for NT compared to the corresponding value before the postharvest treatment. NT showed the highest rate of color change as measured by the ΔE* value compared to 0 days, and the petal color remained unchanged to the greatest degree in the HOCl treatments. Electrolyte leakage from the petals was highest in NT, and there was no petal tissue damage after the HOCl treatments. The flowering rate was lowest in NT, and there were few differences among the treatments. There were no differences among the treatments in terms of the chlorophyll content, Fv/Fm, and stomatal size change rate of the leaf, indicating that the quality of the leaf was not affected. Thus, an immediate petal pulsing treatment with slightly acidic electrolyzed water is recommended to inhibit gray mold on cut rose flowers at 30 µL•L-1 HOCl just before postharvest storage. This treatment did not cause any apparent damage to the petals or leaves.

Biocontrol strategies are gaining tremendous attention with the growing awareness of the irreparable harm caused by the continuous use of synthetic pesticides. This study examines the proteolytic and chitinolytic activities of Bacillus velezensis CE 100 and its termiticidal effect through cuticle degradation. The proteolytic and chitinolytic activities of B. velezensis CE 100 systematically increased with cell growth to the respective peak of 68.3, and 128.3 units/mL after seventh days of inoculation, corresponding with the highest cell growth of 16 x 107 colony forming units (CFU)/mL. The in vitro termiticidal assay showed that B. velezensis CE 100 caused a rapid and high rate of termite mortality with the median lethal time (LT50) of > 1h, and the highest mortality rate of 91.1% and 92.2% recorded at 11h and 12h in the bacterial broth culture and crude enzyme fraction, respectively. In addition to broken setae and deformed sockets, termites treated with the bacterial broth culture exhibited degraded epicuticles, while the crude enzyme fraction caused severe disintegration of both the epicuticle and endocuticle. These results indicate a tremendously higher prospect of B. velezensis CE 100 in the biological control of subterranean termites compared to the previously used entomopathogenic bacteria.

This study investigates the institutional dynamics behind the phenomenon of apartment construction within urban parks in South Korea, using the Policy Arrangement Approach (PAA) as an analytical framework. By comparing two landmark policies—the Citizens Apartments Construction Project (CACP69) of 1969 and the Private Park Special Project (PPSP09) initiated in 2009—it will reveal how park erosion has been legitimized through shifting governance structures, legal mechanisms, and discursive strategies. CACP69 emerged under authoritarian rule to address housing shortages, leveraging public authority to repurpose park land. In contrast, PPSP09 reflects neoliberal tendencies, incentivizing private capital to develop unexecuted park sites amid looming sunset clauses. Both cases illustrate how the long-term neglect of park creation and ambiguous legal definitions have enabled the commodification of public green spaces. The study argues that park management policies in Korea have been shaped by a property-rights-oriented logic, sidelining community participation and ecological integrity. These findings offer critical insights into the intersection of urban planning, environmental governance, and the privatization of public space.