Legumes have important nutritional and economic values, but their production faces continuous cropping obstacles that seriously affect their yield formation. In order to reduce the negative impact of the continuous cropping obstacles of legumes, it is necessary to understand the response mechanisms of legumes to continuous cropping, the causes of continuous cropping obstacles and the measures to alleviate continuous cropping obstacles. This review aimed to identify the current knowledge gap in the field of continuous cropping obstacles of legumes and provide direction and focus for future research. The continuous cropping obstacles of legumes start with soil degradation, leading to oxidative stress in the plants. This triggers the expression of plant-hormone- and signal-molecule-related genes, activating the defense system and causing continuous cropping obstacles. Although there has been progress in researching these challenges in legume crops, many questions remain. We believe that the exploration of molecular mechanisms of legume crops responding to continuous cropping, rhizosphere signal exchange and soil environment repair mechanisms after long-term continuous cropping of soybean, and the excavation of candidate genes and functional loci related to continuous cropping obstacles in legume crops are breakthroughs for proposing effective continuous cropping obstacle management strategies in the future.

Conservation tillage is widely recognized as an important way to improve soil quality, ensure food security and mitigate climate change. However, relatively little attention has been paid to the subject in terms of sustainable evaluation of environmental and economic benefits of the combination of no tillage and straw returning for maize production in arid irrigated areas. In this study, grain yield (GY) and water use efficiency based on grain yield (WUE_GY), soil carbon emission characteristics and economic benefits were investigated, and a sustainability evaluation index based on the above indicators was assessed in maize production under a wheat–maize rotation system from 2009 to 2012. Four wheat straw returning approaches were designed: no tillage with 25 to 30 cm tall wheat straw mulching (NTSMP), no tillage with 25 to 30 cm tall wheat straw standing (NTSSP), conventional tillage with 25 to 30 cm tall wheat straw incorporation (CTSP), and conventional tillage without wheat straw returning (CTP). The results showed that NTSMP treatment could effectively regulate water consumption characteristics of maize fields and meet the water conditions for high grain yield formation, thus gaining higher GY and WUE_GY. NTSMP increased GY and WUE_GY of maize by 13.7–17.5% and 15.4–16.7% over the CTP treatment, and by 5.6–9.0% and 2.3–11.2% over the CTSP treatment, respectively. Meanwhile, compared with CTP, the NTSMP treatment could effectively reduce carbon emissions from maize fields, where average soil carbon emission fluxes (AC_f), carbon emission (CE) and water use efficiency based on carbon emission (WUE_CE) were reduced by 17.7–18.9%, 11.1–11.2% and 8.8–12.8% and carbon emission efficiency (CEE) was increased by 10.2–14.7%. In addition, the NTSMP and NTSSP treatments could effectively increase total output and reduce human labor and farm machinery input, resulting in higher economic benefit. Among them, the NTSMP treatment was the most effective, net income (NI) and benefit per cubic meter of water (BPW) were increased by 16.1–34.2% and 19.1–31.8% over the CTP treatment, and by 13.2–13.3% and 9.8–15.6% over the CTSP treatment, respectively. The sustainability analysis showed that the NTSMP treatment had a high sustainability evaluation index and was a promising field-management strategy. Therefore, no tillage with 25 to 30 cm tall wheat straw mulching is a sustainable maize-management practice for increasing economic benefits and improving environmental impacts in arid irrigated areas.

Soil autotoxic chemosensory substances have emerged as the predominant environmental factors constraining the growth, quality, and yield of Codonopsis pilosula in recent years. Among a vast array of chemosensory substances, benzoic acid constitutes the principal chemosensory substance in the successive cultivation of C. pilosula. However, the exploration regarding the stress exerted by benzoic acid on the growth and development of C. pilosula remains indistinct, and there is a scarcity of research on the mechanism of lobetyolin synthesis in C. pilosula. In the current research, it was discovered that exposure to benzoic acid at a concentration of 200 mmol/L conspicuously attenuated the plant height, root length, total length, fresh weight, root weight, root thickness, chlorophyll content, electrolyte osmolality, leaf intercellular CO₂ concentration (C_i), net photosynthesis rate (P_n), transpiration rate (T_r), and leaf stomatal conductance (G_s) of C. pilosula. Benzoic acid (200 mmol/L) significantly enhanced the activity of root enzymes, including superoxide dismutase (SOD), malondialdehyde (MDA), and peroxidase (POD), as well as the accumulation of polysaccharides and lobetyolins (polyacetylene glycosides) in the roots of C. pilosula. In this study, 58,563 genes were assembled, and 7946 differentially expressed genes were discovered, including 4068 upregulated genes and 3878 downregulated genes. The outcomes of the histological examination demonstrated that benzoic acid stress augmented the upregulation of genes encoding key enzymes implicated in the citric acid cycle, fatty acid metabolism, as well as starch and sucrose metabolic pathways. The results of this investigation indicated that a moderate amount of benzoic acid could enhance the content of lobetyolin in C. pilosula and upregulate the expression of key coding genes within the signaling cascade to improve the resilience of C. pilosula lobetyolin against benzoic acid stress; this furnished a novel perspective for the study of C. pilosula lobetyolin as a potential substance for alleviating benzoic acid-induced stress.

Agricultural soils are the largest anthropogenic source of nitrous oxide (N₂O), primarily due to excessive nitrogen (N) fertilization and inefficient N management. Mitigating N₂O emissions from croplands without compromising productivity is therefore a major global challenge for climate and environmental sustainability. A three-year split-plot field experiment was conducted in an arid maize production region of northwestern China to examine how green manure intercropping combined with reduced chemical N input regulates N₂O emissions and soil N residues. The main plots comprised maize monoculture (M), maize intercropped with common vetch (M/V), and maize intercropped with rape (M/R), while subplots consisted of local conventional N application (N1: 360 kg N ha⁻¹) and a 25% reduced rate (N2: 270 kg N ha⁻¹). Results indicated that intercropping with green manure can offset the reduction in maize grain yield caused by a 25% decrease in N supply. Green manure intercropping significantly decreased cumulative N₂O emissions compared with monoculture maize, and the mitigation effect was further strengthened under reduced N input. The M/V system under reduced N input exhibited the strongest mitigation effect, reducing N₂O emissions per unit of grain yield by 9.2–11.5% compared with the M/R system. This reduction was driven by the ability of M/V to stabilize soil mineral N availability. Notably, the independent maize growth stage contributed 52.6–66.9% of total seasonal N₂O emissions, emphasizing it as a critical period for emission mitigation. Overall, integrating green manure intercropping with reduced chemical N input effectively mitigates N₂O emissions while maintaining maize productivity in arid regions, providing a practical strategy for sustainable and environmentally responsible agricultural intensification.