Leaf nitrogen concentration (LNC) is a primary indicator of crop nitrogen status, closely related to the growth and development dynamics of crops. Accurate and efficient monitoring of LNC is significant for precision field crop management and enhancing crop productivity. However, the biochemical properties and canopy structure of wheat change across different growth stages, leading to variations in spectral responses that significantly impact the estimation of wheat LNC. This study aims to investigate the construction of feature combination indices (FCIs) sensitive to LNC across multiple wheat growth stages, using remote sensing data to develop an LNC estimation model that is suitable for multiple growth stages. The research employs UAV multispectral remote sensing technology to acquire canopy imagery of wheat during the early (Jointing stage and Booting stage) and late (Early filling and Late filling stages) in 2021 and 2022, extracting spectral band reflectance and texture metrics. Initially, twelve sensitive spectral feature combination indices (SFCIs) were constructed using spectral band information. Subsequently, sensitive texture feature combination indices (TFCIs) were created using texture metrics as an alternative to spectral bands. Machine learning algorithms, including partial least squares regression (PLSR), random forest regression (RFR), support vector regression (SVR), and Gaussian process regression (GPR), were used to integrate spectral and texture information, enhancing the estimation performance of wheat LNC across growth stages. Results show that the combination of Red, Red edge, and Near-infrared bands, along with texture metrics such as Mean, Correlation, Contrast, and Dissimilarity, has significant potential for LNC estimation. The constructed SFCIs and TFCIs both enhanced the responsiveness to LNC across multiple growth stages. Additionally, a sensitive index, the Modified Vegetation Index (MVI), demonstrated significant improvement over NDVI, correcting the over-saturation concerns of NDVI in time-series analysis and displaying outstanding potential for LNC estimation. Spectral information outperforms texture information in estimation capability, and their integration, particularly with SVR, achieves the highest precision (coefficient of determination (R²) = 0.786, root mean square error (RMSE) = 0.589%, and relative prediction deviation (RPD) = 2.162). In conclusion, the sensitive FCIs developed in this study improve LNC estimation performance across multiple growth stages, enabling precise monitoring of wheat LNC. This research provides insights and technical support for the construction of sensitive indices and the precise management of nitrogen nutrition status in field crops.

The quality of the image data and the potential to invert crop growth parameters are essential for effectively using unmanned aerial vehicle (UAV)-based sensor systems in precision agriculture (PA). However, the existing research falls short in providing a comprehensive examination of sensor data quality and the inversion potential of crop growth parameters, and there is still ambiguity regarding how the quality of data affects the inversion potential. Therefore, this study explored the application potential of RGB and multispectral (MS) images acquired from three lightweight UAV platforms in the realm of PA: the DJI Mavic 2 Pro (M2P), Phantom 4 Multispectral (P4M), and Mavic 3 Multispectral (M3M). The reliability of pixel-scale data quality was evaluated based on image quality assessment metrics, and three winter wheat growth parameters, above-ground biomass (AGB), plant nitrogen content (PNC) and soil and plant analysis development (SPAD), were inverted using machine learning models based on multi-source image features at the plot scale. The results indicated that the RGB image quality from the M3M outperformed that of the M2P, while the MS image quality was marginally superior to that of the P4M. Nevertheless, these advantages in pixel-scale data quality did not improve inversion accuracy for crop parameters at the plot scale. Spectral features (SFs) derived from the P4M-based MS sensor demonstrated significant advantages in AGB inversion (R² = 0.86, rRMSE = 27.47%), while SFs derived from the M2P-based RGB camera exhibited the best performance in SPAD inversion (R² = 0.60, rRMSE = 7.67%). Additionally, combining spectral and textural features derived from the P4M-based MS sensor yielded the highest accuracy in PNC inversion (R² = 0.82, rRMSE = 14.62%). This study clarified the data quality of three prevalent UAV mounted sensor systems in PA and their influence on parameter inversion potential, offering guidance for selecting appropriate sensors and monitoring key crop growth parameters.

The timely and accurate estimation of above-ground biomass (AGB) is crucial for indicating crop growth status, assisting management decisions, and predicting grain yield. Unmanned aerial vehicle (UAV) remote sensing technology is a promising approach for monitoring crop biomass. However, the determination of winter wheat AGB based on canopy reflectance is affected by spectral saturation effects. Thus, constructing a generic model for accurately estimating winter wheat AGB using UAV data is significant. In this study, a three-dimensional conceptual model (3DCM) for estimating winter wheat AGB was constructed using plant height (PH) and fractional vegetation cover (FVC). Compared with both the traditional vegetation index model and the traditional multi-feature combination model, the 3DCM yielded the best accuracy for the jointing stage (based on RGB data: coefficient of determination (R²) = 0.82, normalized root mean square error (nRMSE) = 0.2; based on multispectral (MS) data: R² = 0.84, nRMSE = 0.16), but the accuracy decreased significantly when the spike organ appeared. Therefore, the spike number (SN) was added to create a new three-dimensional conceptual model (n3DCM). Under different growth stages and UAV platforms, the n3DCM (RGB: R² = 0.73–0.85, nRMSE = 0.17–0.23; MS: R² = 0.77–0.84, nRMSE = 0.17–0.23) remarkably outperformed the traditional multi-feature combination model (RGB: R² = 0.67–0.88, nRMSE = 0.15–0.25; MS: R² = 0.60–0.77, nRMSE = 0.19–0.26) for the estimation accuracy of the AGB. This study suggests that the n3DCM has great potential in resolving spectral errors and monitoring growth parameters, which could be extended to other crops and regions for AGB estimation and field-based high-throughput phenotyping.

Plant nitrogen concentration (PNC) is a key indicator reflecting the growth and development status of plants. The timely and accurate monitoring of plant PNC is of great significance for the refined management of crop nutrition in the field. The rapidly developing sensor technology provides a powerful means for monitoring crop PNC. Although RGB images have rich spatial information, they lack the spectral information of the red edge and near infrared bands, which are more sensitive to vegetation. Conversely, multispectral images offer superior spectral resolution but typically lag in spatial detail compared to RGB images. Therefore, the purpose of this study is to improve the accuracy and efficiency of crop PNC monitoring by combining the advantages of RGB images and multispectral images through image-fusion technology. This study was based on the booting, heading, and early-filling stages of winter wheat, synchronously acquiring UAV RGB and MS data, using Gram–Schmidt (GS) and principal component (PC) image-fusion methods to generate fused images and evaluate them with multiple image-quality indicators. Subsequently, models for predicting wheat PNC were constructed using machine-selection algorithms such as RF, GPR, and XGB. The results show that the RGB_B1 image contains richer image information and more image details compared to other bands. The GS image-fusion method is superior to the PC method, and the performance of fusing high-resolution RGB_B1 band images with MS images using the GS method is optimal. After image fusion, the correlation between vegetation indices (VIs) and wheat PNC has been enhanced to varying degrees in different growth periods, significantly enhancing the response ability of spectral information to wheat PNC. To comprehensively assess the potential of fused images in estimating wheat PNC, this study fully compared the performance of PNC models before and after fusion using machine learning algorithms such as Random Forest (RF), Gaussian Process Regression (GPR), and eXtreme Gradient Boosting (XGB). The results show that the model established by the fusion image has high stability and accuracy in a single growth period, multiple growth periods, different varieties, and different nitrogen treatments, making it significantly better than the MS image. The most significant enhancements were during the booting to early-filling stages, particularly with the RF algorithm, which achieved an 18.8% increase in R², a 26.5% increase in RPD, and a 19.7% decrease in RMSE. This study provides an effective technical means for the dynamic monitoring of crop nutritional status and provides strong technical support for the precise management of crop nutrition.

Timely and accurate yield estimation is essential for effective crop management and the grain trade. Remote sensing has emerged as a valuable tool for monitoring rice yields; however, many studies concentrate on a single period or simply aggregate multiple periods, neglecting the complexities underlying yield formation. The study enhances yield estimation by integrating cumulative time series vegetation indices (VIs) from multispectral (MS) and RGB (Red, Green, Blue) sensors to identify optimal combinations of growth periods. We utilized two unmanned aerial vehicle to capture spectral information from rice canopies through MS and RGB sensors. By analyzing the correlations between vegetation indices from different sensors and rice yields, the optimal MS-VIs and RGB-VIs for each period were identified. Following this, the relationship between the cumulative time series of MS-VIs, RGB-VIs, and rice yields was further examined. The results demonstrate that the booting stage is a crucial growth period influencing rice yield, with VIs exhibiting increased correlation with yield, peaking during this stage before declining. For the MS sensor, the rice yield model, based on the cumulative time series of MS-VIs from the tillering stage to the panicle initiation stage, achieves optimal accuracy (R² = 0.722, RRMSE = 0.555). For the RGB sensor, the rice yield model, based on the cumulative time series of RGB-VIs from the tillering stage to the grain-filling stage, yields the highest accuracy (R² = 0.727, RRMSE = 0.526). In comparison, the multi-sensor rice yield model, which combines the cumulative time series of MS-VIs from the tillering stage and RGB-VIs from the panicle initiation to grain-filling stages, achieves the highest accuracy with R² = 0.759 and RRMSE = 0.513. These findings suggest that cumulative time series VIs and the integration of multiple sensors enhance yield prediction accuracy, providing a comprehensive approach for estimating rice yield dynamics and supporting precision agriculture and informed crop management.

Chlorophyll is a crucial indicator for monitoring crop growth and assessing nutritional status. Hyperspectral remote sensing plays an important role in precision agriculture, offering a non-destructive approach to predicting leaf chlorophyll. However, crop canopy spectra often face background noise and data redundancy challenges. To tackle these issues, this study develops an integrated processing strategy incorporating multiple preprocessing techniques, sequential module fusion, and feature mining methods. Initially, the original spectrum (OS) from 2021, 2022, and the fusion year underwent preprocessing through Fast Fourier Transform (FFT) smoothing, multiple scattering correction (MSC), the first derivative (FD), and the second derivative (SD). Secondly, feature mining was conducted using Competitive Adaptive Reweighted Sampling (CARS), Iterative Retention of Information Variables (IRIV), and Principal Component Analysis (PCA) based on the optimal preprocessing order module fusion data. Finally, Partial Least Squares Regression (PLSR) was used to construct a prediction model for winter wheat SPAD to compare the prediction effects in different years and growth stages. The findings show that the preprocessing sequential module fusion of FFT-MSC (firstly pre-processing using FFT, and secondly secondary processing of FFT spectral data using MSC) effectively reduced issues such as noisy signals and baseline drift. The FFT-MSC-IRIV-PLSR model (based on the combined FFT-MSC preprocessed spectral data, feature screening using IRIV, and then combining with PLSR to construct a prediction model) predicts SPAD with the highest overall accuracy, with an R² of 0.79–0.89, RMSE of 4.51–5.61, and MAE of 4.01–4.43. The model performed best in 2022, with an R² of 0.84–0.89 and RMSE of 4.51–6.74. The best prediction during different growth stages occurred in the early filling stage, with an R² of 0.75 and RMSE of 0.58. On the basis of this research, future work will focus on optimizing the data processing process and incorporating richer environmental data, so as to further enhance the predictive capability and applicability of the model.