The valorization of Crocus sativus focuses mainly on the plant’s stigma because it is one of the most expensive and valuable spices in the world and has great value in the food, cosmetics, and pharmacological industries. Due to this high stigma value, the other parts of the plant are considered as by-products; our study aimed to evaluate the myorelaxant and antispasmodic activities of the byproducts (tepals and leaves) to compare them with the stigma of the plant. To investigate the myorelaxant and antispasmodic activities of the Crocus sativus on isolated rabbit and rat jejunum, we used an in vitro technic with an organ bath and an isotonic transducer. Our results showed that the STG (hydroethanolic extract of stigmas) and LV (hydroethanolic extract of leaves) had a moderate myorelaxant effect with IC₅₀ = 6.61 ± 1.5 and 5.08 ± 0.45 mg/ml, respectively. TPL (hydroethanolic extract of tepals) had a significant () inhibitory effect on the amplitude of the rabbit jejunum basic contractions with an IC₅₀ = 1.36 ± 0.15 mg/ml. TPL also caused a significant () antispasmodic activity depending on the dose of the contraction induced by CCh (10⁻⁶ M) and KCl (25 mM). The antispasmodic effect of the TPL is slightly altered in the presence of nifedipine by a percentage of 26.8%. This difference is statistically significant (). Therefore, the extract could induce the inhibitory effect on L-type voltage-dependent Ca²⁺ channels, but not on the guanylate cyclase and nitric oxide pathways. That confirmed that the TPL has a comparable effect to the verapamil. The HPLC-DAD analysis of various parts of C. sativus shows that the three extracts contain the kaempferol flavanol compound, the STG also was revealed to be rich in carotenoids crocin, and these isomers are trans and cis-crocin, safranal, and picrocrocin. In contrast, the TPL revealed the presence of isorhamnetin and quercetin, but the LV was rich in hesperidin and mangiferin. In conclusion, this study supports the traditional use of this plant to treat digestive problems and will allow us to explore future possibilities for treating bowel spasms using natural molecules derived from saffron.

Background: This study investigated the hypolipidemic and hepatoprotective effects of refined soybean oil supplemented with an Ocimum basilicum L. extract, characterized by HPLC and found to be rich in caftaric, caffeic, chicoric, and rosmarinic acids. Methods: After a 12-week model of diet-induced hyperlipidemia, we examined the plasma levels of TC, TG, Glucose, HDL-C, and LDL-C and the LDL-C/HDL-C ratio using enzymatic kits. The Plasma Hepatic and Biliary Marker Analysis was analysed following standardized hospital protocols with quality-controlled instrumentation. Results: The supplementation with Basil-Enriched Oil (BEO) resulted in a notable redistribution of lipids, significantly reducing the plasma total cholesterol (−75%), triglycerides (−96%), and glucose (−22%), while enhancing their hepatic sequestration. This was accompanied by a marked improvement in the LDL-C/HDL-C ratio and a reduction in hepatic oxidative stress (measured by MDA). Importantly, BEO preserved liver structure and prevented steatosis, despite inducing an increase in adaptive hepatomegaly. Conclusions: The results reveal a dual mechanism whereby the antioxidant properties of BEO collaborate with reprogrammed lipid metabolism, promoting safe hepatic storage rather than harmful circulating levels. These findings strongly advocate for the extract’s potential as a nutraceutical for addressing hyperlipidemia and related metabolic disorders by targeting both oxidative stress and lipid imbalance. Further research is required to confirm these effects in clinical settings and to confirm its long-term efficacy.

Oxidative stress and inflammation play crucial roles in skeletal muscle atrophy and injury, impacting protein synthesis, proteolysis, and muscle regeneration. While inflammation is essential for muscle healing, excessive inflammatory responses can disrupt recovery. Various herbal bioactives have shown promise in modulating these processes. Camellia sinensis, Curcuma longa, Panax ginseng, and several other plants exhibit anti-inflammatory effects by reducing reactive oxygen species production, inhibiting cyclooxygenase activity, and downregulating inflammatory mediators such as IL-1, IL-6, and TNF-α. These plant-derived compounds can potentially optimize muscle healing and prevent atrophy. However, the use of anti-inflammatory drugs may have both beneficial and detrimental effects on muscle adaptations to exercise and training. Understanding the complex interplay between inflammation, oxidative stress, and muscle physiology is crucial for developing effective treatments for muscle-related disorders.

Background: Hyperlipidemia is a major risk factor for cardiometabolic disorders, including non-alcoholic fatty liver disease (NAFLD), and is closely associated with oxidative stress. Opuntia ficus-indica (OFI) cladodes are recognized as a rich source of bioactive phytochemicals; however, the molecular mechanisms underlying their metabolic benefits remain incompletely understood. Objectives: This study aimed to comprehensively evaluate the hypolipidemic and hepatoprotective potential of a polyphenol-rich O. ficus-indica cladode extract (OCE) using an integrated approach combining in vivo evaluation, untargeted metabolomics (UHPLC-Orbitrap-MS/MS), molecular docking, and ADMET prediction. Methods: Hyperlipidemic mice fed a high-fat diet (HFD) were treated with OCE, while molecular docking was performed on ten major annotated phytochemicals against twelve key proteins involved in lipid metabolism and cholesterol homeostasis, including HMGCR, FAS, PPARα, PCSK9, and NPC1L1, using simvastatin as the reference compound. Results: OCE treatment significantly improved plasma and hepatic lipid profiles, improved glucose homeostasis, and markedly reduced hepatic malondialdehyde (MDA) levels, indicating attenuation of oxidative stress. Histopathological analysis further supported a pronounced hepatoprotective effect, with a substantial reduction in hepatic steatosis. Untargeted metabolomics enabled the annotation of 102 metabolites, putatively identifying piscidic acid as the predominant phenolic constituent together with a diverse profile of flavonoids and phenolic acids. Molecular docking supported the potential contribution of these phytochemicals to the regulation of lipid metabolism through favorable interactions with multiple therapeutic targets, while ADMET prediction suggested an overall favorable pharmacokinetic and toxicity profile despite the lower intestinal permeability predicted for glycosylated derivatives. Conclusions: Overall, these findings support O. ficus-indica cladodes as a promising source of dietary bioactive compounds with potential applications in the nutritional management and prevention of hyperlipidemia and related cardiometabolic disorders.