Li ming Liu

Dr. Li ming Liu


Research Focus & Metrics

Academic Career Length

25Years (2002-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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13 January 2015 in BMC Systems Biology
Verified
Journal Article
Open access
PDF file
CYChao Ye
Chao Ye
NXNan Xu
Nan Xu
HCHaiqin Chen
Haiqin Chen
YCYongquan Chen
Yongquan Chen
WCWei Chen
Wei Chen
LLLi ming Liu
Li ming Liu
Mortierella alpina is an oleaginous fungus used in the industrial scale production of arachidonic acid (ARA). In order to investigate the metabolic characteristics at a systems level and to explore potential strategies for enhanced lipid production, a genome-scale metabolic model of M. alpina was reconstructed. This model included 1106 genes, 1854 reactions and 1732 metabolites. On minimal growth medium, 86 genes were identified as essential, whereas 49 essential genes were identified on yeast extract medium. A series of sequential desaturase and elongase catalysed steps are involved in the synthesis of polyunsaturated fatty acids (PUFAs) from acetyl-CoA precursors, with concomitant NADPH consumption, and these steps were investigated in this study. Oxygen is known to affect the degree of unsaturation of PUFAs, and robustness analysis determined that an oxygen uptake rate of 2.0 mmol gDW−1 h−1 was optimal for ARA accumulation. The flux of 53 reactions involving NADPH was significantly altered at different ARA levels. Of these, malic enzyme (ME) was confirmed as a key component in ARA production and NADPH generation. When using minimization of metabolic adjustment, a knock-out of ME led to a 38.28% decrease in ARA production. The simulation results confirmed the model as a useful tool for future research on the metabolism of PUFAs.

26 April 2017 in Chemical Reviews
Verified
Journal Article
Closed access
XCXiulai Chen
Xiulai Chen
CGCong Gao
Cong Gao
LGLiang Guo
Liang Guo
GHGuipeng Hu
Guipeng Hu
QLQiuling Luo
Qiuling Luo
LLLi ming Liu
Li ming Liu

1 June 2014 in Trends in Biotechnology
Verified
Journal Article
Closed access
XCXiulai Chen
Xiulai Chen
SLShubo Li
Shubo Li
LLLi ming Liu
Li ming Liu

29 April 2021 in Nature Catalysis
Verified
Journal Article
Closed access
PDF file
GHGuipeng Hu
Guipeng Hu
ZLZehong Li
Zehong Li
DMDanlei Ma
Danlei Ma
CYChao Ye
Chao Ye
LZLinpei Zhang
Linpei Zhang
XCXiulai Chen
Xiulai Chen

1 May 2019 in Trends in Biotechnology
Verified
Journal Article
Closed access
GHGuipeng Hu
Guipeng Hu
YLYin Li
Yin Li
CYChao Ye
Chao Ye
LLLi ming Liu
Li ming Liu
XCXiulai Chen
Xiulai Chen

1 May 2019 in Metabolic Engineering
Verified
Journal Article
Closed access
YQYanli Qi
Yanli Qi
HLHui Liu
Hui Liu
XCXiulai Chen
Xiulai Chen
LLLi ming Liu
Li ming Liu