Heavy metal pollution is one of the most serious environmental and human health risk problem associated with industrial progress. The present study was conducted with the goal of isolation and characterization of metal-resistant lactic acid bacteria (LAB) from the Alexandrian Mediterranean Seacoast, Egypt, with their possible exploitation in metal remediation. Lactobacillus plantarum MF042018 exhibited high degree of resistance, up to 500 and 100 ppm, to both nickel and chromium, respectively, with multiple antibiotic resistance (MAR) index above 0.5. In an attempt to improve chromium removal by L. plantarum MF042018, Plackett-Burman followed by Box-Behnken statistical designs were applied. An initial Cr²⁺ concentration of 100 ppm and inoculum size of 3% presented the best conditions for the accumulation of chromium by L. plantarum MF042018. The study was also navigated to assess the biosorption capacity of L. plantarum MF042018, the maximum uptake capacity (q) of both Cd²⁺ and Pb²⁺ was recorded at pH 2.0 and a temperature of 22 °C after 1 hr. The biosorption process of Cd²⁺ and Pb²⁺ was well explained by the Langmuir isotherm model better than the Freundlich isotherm. Furthermore, the results revealed that the use of L. plantarum MF042018 is an effective tool for the treatment of hazardous metal-polluted battery-manufacturing effluent. Therefore, the present study implies that L. plantarum MF042018 can be applied as a promising biosorbent for the removal of heavy metals from industrial wasterwaters.

Microbial bioremediation of heavy metals-polluted industrial effluents has been adopted as one of the most effective eco-friendly tool to cope up with the harmful effects of metals. This study was designed to investigate the biosorption potential of marine actinomycetes isolated from the Alexandrian Mediterranean Seacoast, Egypt, with their potential use in metal remediation of industrial effluents. Among the nine marine actinomycetes isolates, Streptomyces rochei ANH showed the highest versatile metal resistance capability with MIC values of 125 mg/l for Cr⁶⁺ and 60 mg/l for both Cd²⁺ and Pb²⁺. Additionally, scanning electron micrographs showed complete disintegration of Cr⁶⁺-treated biomass compared with the control ones where spores remained intact and connected in long chains. The study also aimed to improve the percentage of Cr⁶⁺ biosorption by S. rochei ANH biomass using the statistical designs of Plackett–Burman and Box-Behnken where up to 85% of Cr⁶⁺ removal was recorded under the following conditions: pH (5), incubation temperature (30 °C), contact time (3 h), agitation speed (90 rpm), initial Cr⁶⁺ concentration (50 mg/l) and living biomass concentration (10 mg/ml). The results also showed that the percentage of Cr⁶⁺ biosorption by S. rochei ANH decreased gradually beyond these values. Moreover, the results revealed that the use of the biomass of S. rochei ANH is an effective biotechnological agent for the biological treatment of heavy metal-contaminated tannery effluent where the percentages of metal removal were in the following order: Ni²⁺ (100%) ≥ Cu²⁺ ≥ Mn²⁺ ≥ Fe²⁺ > Pb²⁺ (95%) ≥ Cd²⁺ > Cr⁶⁺ (86%). Furthermore, the treated effluent exhibited a stimulating effect on the germination process of Lepidium sativum seeds. Therefore, the present study implies that S. rochei ANH can be considered a powerful candidate to mitigate hazardous heavy metals pollution from industrial effluents and improve the water quality for agricultural purposes.