In respiratory diseases, the induction of allergic asthma is one of the hottest issues of international concern. The adjuvant effect of air pollutants including nanoparticles (NPs) has be pointed out to facilitate the occurrence and development of allergic asthma. This work studied the development of allergic asthma upon exposures of carbon black nanoparticles (CB NPs, 30-50 nm) and/or high environmental humidity (90% relative humidity). The mechanisms involved were investigated from perspectives of the activation of oxidative stress and transient receptor potential vanilloid 1 (TRPV1) pathways and the alteration in intestinal microbiota. Both high humidity and CB NPs aggravated the airway hyperreactivity, remodeling, and inflammation in Balb/c mice sensitized by ovalbumin. The co-exposure of these two risk factors exhibited adjuvant effect on the development of asthma likely through activating oxidative stress pathway and TRPV1 pathway and then facilitating type I hypersensitivity. Additionally, exposures of high humidity and/or CB NPs reduced the richness of intestinal microbes, altered microbial community composition, and weakened corresponding biological functions, which may interact with the development of asthma. The findings will add new toxicological knowledge to the health risk assessment and management of co-exposures of NPs and other risk factors in the environment.

In the context of global climate change, organisms in their natural habitats usually suffer from unfavorable climatic conditions together with environmental pollution. Temperature and humidity (or moisture) are two central climatic factors, while their relationships with the toxicity of contaminants are not well understood. This review provides a synthesis of existing knowledge on important interactions between contaminant toxicity and climatic conditions of unfavorable temperature, soil moisture, and air humidity. Both high temperature and low moisture can extensively pose severe combined hazards with organic pollutants, heavy metal ions, nanoparticles, or microplastics. There is more information on the combined effects on animalia than on other kingdoms. Prevalent mechanisms underlying their joint effects include the increased bioavailability and bioaccumulation of contaminants, modified biotransformation of contaminants, enhanced induction of oxidative stress, accelerated energy consumption, interference with cell membranes, and depletion of bodily fluids. However, the interactions of contaminants with low temperature or high humidity/moisture, particularly on plants and microorganisms, are relatively vague and need to be further revealed. This work emphasizes that the co-exposure of chemical and physical stressors results in detrimental effects generally greater than those caused by either stressor. It is necessary to take this into consideration in the ecological risk assessment of both environmental contamination and climate change.

Asthma is a chronic respiratory disease that is highly sensitive to environmental pollutants, including engineered nanoparticles (NPs). Exposure to NPs has become a growing concern for human health, especially for susceptible populations. Toxicological studies have demonstrated strong associations between ubiquitous NPs and allergic asthma. In this review, we analyze articles that focus on adverse health effects induced by NPs in animal models of allergic asthma to highlight their critical role in asthma. We also integrate potential mechanisms that could stimulate and aggravate asthma by NPs. The toxic effects of NPs are influenced by their physicochemical properties, exposure dose, duration, route, as well as the exposure order between NPs and allergens. The toxic mechanisms involve oxidative stress, various inflammasomes, antigen presenting cells, immune cells, and signaling pathways. We suggest that future research should concentrate on establishing standardized models, exploring mechanistic insights at the molecular level, assessing the combined effects of binary exposures, and determining safe exposure levels of NPs. This work provides concrete evidence of the hazards posed by NPs in animals with compromised respiratory health and supports the modifying role of NPs exposure in allergic asthma. Graphical Abstract: