Chemical investigations on ethanolic extract of Argyrolobium roseum led to the isolation of Pinitol as the major constituent of the plant. Pinitol is chemically known as 3-O-methyl-D-Chiro-inositol and has been found to possess anti-diabetic activity. It helps in the regeneration of beta cells, present in the areas of the pancreas called as islets – of Langerhans. These cells make and release insulin, a hormone which controls the level of glucose in the blood. Pinitol was isolated from the ethanolic extract of the plant and a sensitive & reliable method, based on Proton Nuclear Magnetic Resonance (PNMR), was developed and used as an analytical tool for quantification and identification of this relatively UV insensitive compound in the alcoholic extract of the plant. The method involves the use of pyrazinamide (an anti-tuberculosis drug), as a reference. Validation of the method was carried out by preparing a known concentration of an artificial mixture of pinitol and pyrazinamide. The recovery of pinitol in the mixture was in the range of 98.5–101.3%. Pinitol in pure form was isolated from the ethanolic extract of A. roseum by repeated column chromatography over silica gel followed by crystallization in methanol. Pinitol isolated from the plant was identified on the basis of 1H-NMR, 13C-NMR, DEPT (45°, 90° and 135°) experiments and mass spectral data. The method was successfully applied for the quantitation of pinitol in various extracts of the said plant

Boswellia serrata, also known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis. It contains naturally occurring triterpenoic acids, called as boswellic acids (BA’s). A highly reproducible High performance liquid chromatography-ultraviolet diode array detection (HPLC-UV-DAD) method was developed for the simultaneous determination and quantitative analysis of eight major triterpenoic acids in Boswellia serrata gum resin obtained by different extraction techniques. All the calibration curves exhibited good linear regression (R2 > 0.997) within the test ranges. The established method showed good precision and overall recoveries of the boswellic acids. The eight triterpenoic acids coded as BS-1 (11-keto-beta-boswellic acid), BS-2 (3-O-acetyl-11-keto-beta-boswellic acid), BS-3 (3-keto tirucallic acid), BS-4 (3-O-acetyl-alpha-tirucallic acid), BS-5 (3-O-acetyl-beta-tirucallic acid), BS-6 (alpha-boswellic acid), BS-7 (beta-boswellic acid) and BS-8 (3-O-acetyl-beta-boswellic acid) were isolated from the processed gum resin of Boswellia serrata by column chromatography. The proposed HPLC method is simple, reliable and has been very useful for the qualitative as well as quantitative analysis of boswellic acids in the gum resin of Boswellia serrata. The proposed method allows to quantify boswellic acids in appreciable amounts by HPLC-UV (DAD) method in the extracts and the available marketed formulations.

A specific and sensitive UPLC-qTOF-MS/MS method has been developed for the simultaneous determination of curcuminoids. These Curcuminoids comprises of curcumin, a principal curcuminoid and other two namely, demethoxycurcumin, and bisdemethoxycurcumin obtained from rhizomes of Curcuma longa an ancient Indian curry spice turmeric, family (Zingiberaceae).

Two distinct chemotypes of Artemisia dracunculus were found in Kashmir. The oils from these two distinct chemotypes were analysed by GC and GC-MS. The chemical constituents of local variety were α-pinene (0.7%), myrecene (1.2 %), limonene (3.5 %), Z-(β)-ocimene (12.7 %), E-(β)-ocimene (4.6 %), α-terpinolene (2.7 %), 5-phenyl-l,3-Pentadiyne (5.1 %), methyl eugenol (0.7 %), capillene (60.2 %), elemicin (2.3 %), bicyclogermacrene (0.5 %), iso-elemicin (2.1 %) and germacrene-B (0.6 %). The major chemical constituents found in exotic variety are α-pinene (1.4 %), β-pinene (0.3 %), myrecene (0.1 %), p-cymene (0.2 %), limonene (2.8 %), Z-(β)-ocimene (1.6 %), E-(β)-ocimene (1.0 %), γ-terpinene (0.3 %) linalool (1.2 %), β-thujone (0.4 %),camphor (0.4 %), methyl chavicol (71.3 %), iso-menthol (0.1 %), bornyl acetate (0.7 %), carvacraol (7.7 %), α-terpinyl acetate (0.2 %), hexyl hexanoate (0.5 %), α-copaene (2.2 %), β-caryophyllene (0.2 %). The chemical constituents of the volatile oil are similar to French Tarragon.