Food losses, defined as a reduction in the quantity and quality of food during production and storage, impact food safety and security. Losses caused by plant pathogens are among the most significant. Chemical pesticides have been extensively used to prevent microbial diseases. Their toxicity and reduced efficacy, however, have encouraged investigators to develop alternatives. Alternatives based on microbial biopesticides tend to be safer and more environmentally benign than conventional pesticides. In recent years, formulations based on biopesticides have progressively increased in number and diversity and have attracted commercial interest. Understanding the mechanisms by which biopesticides control the disease is fundamental to achieving optimal disease control. Biocontrol mechanisms can be divided into two main categories: those related to the ability to inhibit pathogens or their virulence factors, and those that enhance host plant fitness and induce disease resistance. Here, the first type of strategy is reviewed, which is directly mediated by physical contact between biocontrol agents and pathogens or indirectly by exposure of a pathogen to antimicrobial or microbial-inhibiting compounds produced by the microbial antagonist. Mechanisms involving physical contact include mycophagy, destruction of pathogenic bacteria by bacteriophages or predation, and disease inhibition by topical applications of specific dsRNA. Indirect mechanisms that do not involve direct contact with a pathogen include the production of antimicrobial compounds, competition, and virulence factor suppression by quorum quenching. These topics are reviewed and discussed.
Urbanisation and climate change have intensified the Urban Heat Island (UHI) effect in European cities, increasing thermal stress and health risks, especially for vulnerable people. Nature-based Solutions (NbS), such as green infrastructures (GI), offer effective mitigation strategies. A notable example is the Horizon 2020 URBAN GreenUP project in Valladolid, Spain, where a green shade shelter infrastructure was installed and monitored between 2019 and 2022 to evaluate its cooling performance and its effects on temperature-based urban heat indicators through the use of Key Performance Indicators (KPIs). Local thermal conditions were monitored across two adjacent narrow streets—one with the green shade shelter and one as a control. The installation of the green shade shelter in Valladolid produced measurable thermal benefits. During summer peaks, it reduced ambient temperatures by approximately 0.9 °C, with daily maximum temperatures decreasing by 1.0–3.0 °C. In winter, daily minimum temperatures dropped by around 1.0 °C, while autumn saw an increase of 0.7 °C. Summer minimum temperatures varied, ranging from a 0.2 °C decrease in early summer to a 0.7 °C increase in late summer, with a 0.3 °C rise during peak summer in mid-July. The intervention also reduced the frequency and delayed the onset of days exceeding 35 °C and nights above 20 °C, although the delay in peak tropical nights was limited to seven days. These findings indicate that green shade shelters can contribute to improving street-level thermal conditions and reducing temperature-based heat exposure indicators in dense urban environments.