Plant-derived antimicrobial agents have adequate antimicrobial effects on food-borne pathogens, which can be used as food preservatives. The purpose of this study was to evaluate the antibacterial mechanism of chlorogenic acid (CA) against Yersinia enterocolitica and Enterobacter sakazakii. The minimum inhibitory concentration (MIC) of CA was determined by employing the broth microdilution method. Then, the cell function and morphological changes of Y. enterocolitica and E. sakazakii treated with CA were characterized. Finally, the growth inhibition models of Y. enterocolitica in raw pork and E. sakazakii in skim milk were constructed through the response surface methodology. The results demonstrated that CA has a satisfactory inhibitory effect against Y. enterocolitica and E. sakazakii with a MIC of 2.5 mg/mL. In addition, CA inhibited the growth of Y. enterocolitica and E. sakazakii via cell membrane damage, such as depolarization of the cell membrane, reduction in intracellular adenosine triphosphate (ATP) and pH levels, and destruction of cell morphology. Moreover, CA reduced two log cycles of Y. enterocolitica in raw pork and E. sakazakii in skim milk at a certain temperature. According to the corresponding findings, CA has the potential to be developed as an effective preservative to control Y. enterocolitica and E. sakazakii-associated foodborne diseases.

A series of blend membranes were prepared by immersion precipitation phase inversion method using hydroxypropylcellulose and polyacrylonitrile as raw materials. The effects of casting conditions on membrane’s characterization were studied. The properties of resulting blend membranes were that water flux decreased from 489 L·h ^-1 ·m ^-2 to 312 L·h ^-1 ·m ^-2 ; retention rate increased from 80.1% to 91.8%; and corresponding porosity presented increasing trend but changed little within the range of casting solution concentrations from 10 wt % to 16 wt %. The greater the mass fraction of HPC in casting solution, the greater the water flux of blend membranes. Prolonging the atmosphere exposing time, water flux increased from 268 L·h ^-1 ·m ^-2 about 20s to 372 L·h ^-1 ·m ^-2 about 60 s, then decreased to 340 L·h ^-1 ·m ^-2 about 75s. In addition, retention rate decreased from 91.2% to 81.3%. Porosity tend to decrease but changed a little. With the coagulation bath temperature rising, water flux of blend membranes increased from 306 L·h ^-1 ·m ^-2 about 20 ⁰ C to 429 L·h ^-1 ·m ^-2 about 35 ⁰ C, and corresponding retention rate decreased from 87.9% to 82.5%, porosity decreased from 80.23% to 68.26%.