In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and −19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.

In recent years, interest in the eco-friendly manufacturing of metal nanoparticles from plant extracts has surged. Nonetheless, no research has examined the combined antibacterial and anticancer properties of SeNPs synthesized with Solanum tuberosum (S. tuberosum) extract. This study involved the synthesis of selenium nanoparticles (ST-SeNPs) utilizing phytochemicals with reducing and capturing properties derived from the aqueous extract of S. tuberosum shell through a green synthesis approach. To determine the unique characteristics of ST-SeNPs nanoparticles, a variety of techniques were used, including scanning electron microscopy (SEM), zeta potential analysis, transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), UV-visible (UV-Vis) spectroscopy, and energy dispersive X-ray spectrometry (EDX). The optical characteristics of ST-SeNPs were validated using UV-Vis measurement, revealing the peak absorbance at 350 nm. FTIR examination verified the presence of functional groups on the surface of the produced ST-SeNPs nanoparticles. Upon examination of the SEM results, it was concluded that the synthesized SeNPs exhibited uniform distribution and possessed a round morphology. The anticancer efficacy of the produced nanoparticles on the A549 lung cancer cell line and OVCAR-3 ovarian cancer cell line after 24 and 48 hours of exposure was assessed using the MTT test. It was established that elevated concentration inhibited cell growth. The inhibitory efficacy of SeNPs against the proliferation of Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Candida albicans (C. albicans) was assessed using the disk diffusion agar technique. The evaluated SeNPs exhibited antibacterial efficacy against bacterial and yeast cells. The results indicate that ST-SeNPs produced via green synthesis can serve as anticancer and antibacterial agents.

Objective: This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of Elaeagnus angustifolia L. (E. angustifolia) (oleaster) leaves. Methods: Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method. The free radical scavenging activity was determined by the (2,2-Diphenyl-1-picrylhydrazyl) DPPH method, the free radical scavenging activity was determined by the ABTS method, and cytotoxicity assays were performed by the MTT method in human retinal epithelium cells (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer cells (DU-145) cell lines. Results: High amounts of gallic acid, protocatechuic acid, and o-coumaric acid were identified as phenolic compounds. E. angustifolia was found to have a good antioxidant capacity and high free radical scavenging capacity. In this study, for the first time, E. angustifolia leaf extract was used to investigate cytotoxic effects on human retinal epithelium (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer (DU-145) cells and antimicrobial effects on Listeria monocytogenes American Type Culture Collection (ATTC) 7644, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 11774, and Candida albicans ATCC 10231 microorganisms. The highest cytotoxic effect was observed in the DU-145 cell line, and the highest antimicrobial effect was observed in Listeria monocytogenes ATTC 7644 and Candida albicans ATCC 10231. The leaf extract of the plant contains some important phenolic compounds and has high free radical scavenging capacity, a good anticancer effect, and effective antimicrobial activity on yeast species such as C. albicans. Conclusion: Our study will contribute greatly to the search for anticancer and antimicrobial agents, especially from a pharmacological perspective, by examining biological activity using three different methods.