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P. Garcia
Department of Molecular Biology, Faculty of Biological and Environmental Sciences, University of León, 24007 León, Spain

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Journal article
Published: 13 May 2021 in Water
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Benthic diatoms have traditionally been used as bioindicators of aquatic ecosystems. Because diatom-based monitoring of water quality is required by European legislation, molecular-based methods had emerged as useful alternatives to classical methods based on morphological identification using light microscopy. The aim of this study was to test the reliability of DNA metabarcoding combined with High-Throughput Sequencing (HTS) techniques in the bioassessment of the trophic status of 22 Mediterranean shallow ponds in NW Spain. For each pond, the Trophic Diatom Index (TDI) was calculated from inventories obtained by identification using light microscopy (LM) followed by high-throughput sequencing (HTS) at the molecular level. Ponds were subsequently classified into five water quality classes. The results showed a good correspondence between both methods, especially after applying a correction factor that depended on the biovolume of the cells. This correspondence led to the assignment to the same quality class in 59% of the ponds. The determination and quantification of valves or DNA sequences was one of the main pitfalls, which mainly included those related to the variability in the relative abundances of some species. Accordingly, ponds with similar relative abundances for the dominant species were assigned to the same quality class. Moreover, other difficulties leading the discrepancies were the misidentification of some species due to the presence of semi-cryptic taxa, the incompleteness of the reference database and the bioinformatic protocol. Thus, the validation of DNA-based methods for the identification of freshwater diatoms represents an important goal, as an alternative to using traditional methods in Mediterranean shallow ponds.

ACS Style

María Borrego-Ramos; Eloy Bécares; Pedro García; Alejandro Nistal; Saúl Blanco. Epiphytic Diatom-Based Biomonitoring in Mediterranean Ponds: Traditional Microscopy versus Metabarcoding Approaches. Water 2021, 13, 1351 .

AMA Style

María Borrego-Ramos, Eloy Bécares, Pedro García, Alejandro Nistal, Saúl Blanco. Epiphytic Diatom-Based Biomonitoring in Mediterranean Ponds: Traditional Microscopy versus Metabarcoding Approaches. Water. 2021; 13 (10):1351.

Chicago/Turabian Style

María Borrego-Ramos; Eloy Bécares; Pedro García; Alejandro Nistal; Saúl Blanco. 2021. "Epiphytic Diatom-Based Biomonitoring in Mediterranean Ponds: Traditional Microscopy versus Metabarcoding Approaches." Water 13, no. 10: 1351.

Journal article
Published: 30 April 2021 in Science of The Total Environment
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Diatoms are important organisms in freshwater ecosystems due to their position as primary producers and therefore, analyzing their assemblages provides relevant information on ecosystem functioning. Diatoms have historically been identified based on morphological traits, which is time-consuming and requires well-trained specialists. Nevertheless, DNA barcoding offers an alternative approach to overcome some limitations of the morphological method. Here, we assess if both approaches are comparable methods to study patterns and mechanisms (including environmental filtering and dispersal limitation) of epiphytic diatom metacommunities using a comprehensive dataset from 22 Mediterranean ponds at different taxonomic resolutions. We used a fragment of rbcL barcode gene combined with High-Throughput Sequencing to infer diatom community composition. The overall degree of correspondence between both approaches was assessed by Procrustean rotation analysis and Procrustean randomization tests, whereas the role of local environmental variables and geographical distances was studied using a comprehensive combination of BIOENV, Mantel tests and distance-based redundancy analysis. Our results showed a relatively poor correspondence in the compositional variation of diatom metacommunity between both approaches. We speculate that the incompleteness of the reference database and the bioinformatics processing are the biases most likely affecting the molecular approach, whereas the limited counting effort and the presence of cryptic species are presumably the major biases related with the morphological method. On the other hand, variation in diatom community composition detected with both approaches was strongly related to the environmental template, which may be related with the narrow community-environment relationships in diatoms. Nevertheless, we found no significant relationship between compositional variation and geographical distances. Overall, our work shows the complementary nature of both approaches and highlights the importance of DNA metabarcoding to address empirical research questions of community ecology in freshwaters, especially once the reference databases include most genotypes of occurring taxa and bioinformatics biases are overcome.

ACS Style

Alejandro Nistal-García; Pedro García-García; Jorge García-Girón; María Borrego-Ramos; Saúl Blanco; Eloy Bécares. DNA metabarcoding and morphological methods show complementary patterns in the metacommunity organization of lentic epiphytic diatoms. Science of The Total Environment 2021, 786, 147410 .

AMA Style

Alejandro Nistal-García, Pedro García-García, Jorge García-Girón, María Borrego-Ramos, Saúl Blanco, Eloy Bécares. DNA metabarcoding and morphological methods show complementary patterns in the metacommunity organization of lentic epiphytic diatoms. Science of The Total Environment. 2021; 786 ():147410.

Chicago/Turabian Style

Alejandro Nistal-García; Pedro García-García; Jorge García-Girón; María Borrego-Ramos; Saúl Blanco; Eloy Bécares. 2021. "DNA metabarcoding and morphological methods show complementary patterns in the metacommunity organization of lentic epiphytic diatoms." Science of The Total Environment 786, no. : 147410.

Journal article
Published: 15 February 2020 in Bionatura
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Otosclerosis (OTSC) is a common type of deafness affecting up to 0.4 % of Caucasians. Its familial form is inherited in an autosomal dominant fashion, although to this date, no definitive cause for OTSC has been found. In the development of OTSC, three recent genetic association studies have suggested the participation of particular point mutations and small indels in the TGFB1, SERPINF1, and MEPE genes. Consequently, replicative studies are needed to confirm the role of the proposed mutations in OTSC patients. The goal of this study was to test the presence of the candidate variants described in the genes TGFB1, SERPINF1, and MEPE in a new case of familial OTSC with seven affected individuals. DNA was extracted from saliva samples of a Spanish family with several members affected by OTSC. PCR amplified target regions of some candidate genes, and the products were purified, Sanger-sequenced, and analyzed in silico. The family subject of the study did not carry the candidate variants for OTSC described in the genes TGFB1, SERPINF1, and MEPE, although it cannot be ruled out the involvement of other mutations in genes related to their same signaling pathways. This result highlights the importance of performing replicative studies for complex diseases, such as OTCS, in families of diverse origins. Additionally, a significant association of subjective tinnitus with OTSC has been found in this family, although the link between the two pathologies should be studied further.

ACS Style

Francisco J. Álvarez; Pedro García; Jesús Alonso; Santiago Álvarez. Evaluation of Gene Variants in TGFB1, SERPINF1 and MEPE in a Spanish Family Affected by Otosclerosis and Tinnitus. Bionatura 2020, 5, 1050 -1055.

AMA Style

Francisco J. Álvarez, Pedro García, Jesús Alonso, Santiago Álvarez. Evaluation of Gene Variants in TGFB1, SERPINF1 and MEPE in a Spanish Family Affected by Otosclerosis and Tinnitus. Bionatura. 2020; 5 (1):1050-1055.

Chicago/Turabian Style

Francisco J. Álvarez; Pedro García; Jesús Alonso; Santiago Álvarez. 2020. "Evaluation of Gene Variants in TGFB1, SERPINF1 and MEPE in a Spanish Family Affected by Otosclerosis and Tinnitus." Bionatura 5, no. 1: 1050-1055.

Journal article
Published: 13 February 2020 in Spanish Journal of Agricultural Research
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Aim of study: The purpose was to identify some general and genotypic-specific defense responses, in order to obtain a set of candidate genes presumably involved in the resistance.Area of study: The experiment was carried out in León, Spain.Material and methods: We have analyzed the response of three lentil genotypes to Ascochyta lentis (isolate AL 84) at transcriptomic level using the Massive Analysis of cDNA Ends (MACE) technique: the susceptible cultivar 'Lupa', the moderately resistant 'ILL5588' and the resistant wild accession 'BG 16880' (L. culinaris subsp. orientalis).Main results: MACE results yielded a total of 50,935 contigs. The average number of detected contigs in each of the six samples was approximately of 40,000. In response to Ascochyta infection, the jasmonic acid pathway and the lignin biosynthesis were up-regulated in resistant genotypes, while they were down-regulated in the susceptible one. The response to chitin, the salicylic pathway and the auxin response were activated only in the resistant L. c. culinaris genotype, while the giberellin synthesis was only induced in the susceptible L. c. culinaris cv. 'Lupa'. A set of 18 lentil gene sequences putatively involved in the response to the pathogen were validated by RT-qPCR.Research highlights: It can be concluded that in response to the infection by Ascochyta, the lignin biosynthesis and the JA pathway were critical for the resistance, while the giberellin synthesis seems to be related with susceptibility to the pathogen.

ACS Style

Pedro García-García; Francisca Vaquero; F. Javier Vences; Luis E. Sáenz De Miera; Carlos Polanco; Ana I. González; Ralf Horres; Nicolas Krezdorn; Björn Rotter; Peter Winter; Marcelino Pérez De La Vega. Transcriptome profiling of lentil in response to Ascochyta lentis infection. Spanish Journal of Agricultural Research 2020, 17, e0703 .

AMA Style

Pedro García-García, Francisca Vaquero, F. Javier Vences, Luis E. Sáenz De Miera, Carlos Polanco, Ana I. González, Ralf Horres, Nicolas Krezdorn, Björn Rotter, Peter Winter, Marcelino Pérez De La Vega. Transcriptome profiling of lentil in response to Ascochyta lentis infection. Spanish Journal of Agricultural Research. 2020; 17 (4):e0703.

Chicago/Turabian Style

Pedro García-García; Francisca Vaquero; F. Javier Vences; Luis E. Sáenz De Miera; Carlos Polanco; Ana I. González; Ralf Horres; Nicolas Krezdorn; Björn Rotter; Peter Winter; Marcelino Pérez De La Vega. 2020. "Transcriptome profiling of lentil in response to Ascochyta lentis infection." Spanish Journal of Agricultural Research 17, no. 4: e0703.

Journal article
Published: 02 December 2019 in Scientific Reports
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The degree to which dispersal limitation interacts with environmental filtering has intrigued metacommunity ecologists and molecular biogeographers since the beginning of both research disciplines. Since genetic methods are superior to coarse proxies of dispersal, understanding how environmental and geographic factors influence population genetic structure is becoming a fundamental issue for population genetics and also one of the most challenging avenues for metacommunity ecology. In this study of the aquatic macrophyte Myriophyllum alterniflorum DC., we explored the spatial genetic variation of eleven populations from the Iberian Plateau by means of microsatellite loci, and examined if the results obtained through genetic methods match modern perspectives of metacommunity theory. To do this, we applied a combination of robust statistical routines including network analysis, causal modelling and multiple matrix regression with randomization. Our findings revealed that macrophyte populations clustered into genetic groups that mirrored their geographic distributions. Importantly, we found a significant correlation between genetic variation and geographic distance at the regional scale. By using effective (genetic) dispersal estimates, our results are broadly in line with recent findings from metacommunity theory and re-emphasize the need to go beyond the historically predominant paradigm of understanding environmental heterogeneity as the main force driving macrophyte diversity patterns.

ACS Style

Jorge García-Girón; Pedro García; Margarita Fernández-Aláez; Eloy Bécares; Camino Fernández-Aláez. Bridging population genetics and the metacommunity perspective to unravel the biogeographic processes shaping genetic differentiation of Myriophyllum alterniflorum DC. Scientific Reports 2019, 9, 1 -10.

AMA Style

Jorge García-Girón, Pedro García, Margarita Fernández-Aláez, Eloy Bécares, Camino Fernández-Aláez. Bridging population genetics and the metacommunity perspective to unravel the biogeographic processes shaping genetic differentiation of Myriophyllum alterniflorum DC. Scientific Reports. 2019; 9 (1):1-10.

Chicago/Turabian Style

Jorge García-Girón; Pedro García; Margarita Fernández-Aláez; Eloy Bécares; Camino Fernández-Aláez. 2019. "Bridging population genetics and the metacommunity perspective to unravel the biogeographic processes shaping genetic differentiation of Myriophyllum alterniflorum DC." Scientific Reports 9, no. 1: 1-10.

Research article
Published: 27 March 2019 in PLoS ONE
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Usage of high-throughput sequencing approaches allow for the generation and characterization of reference transcriptome datasets that support gene-based marker discovery, which in turn can be used to build genetic maps among other purposes. We have obtained a transcriptome assembly including 49,453 genes for the lentil (Lens culinaris Medik.) cultivar Alpo using RNAseq methodology. This transcriptome was used as reference to obtain 6,306 quality polymorphic markers (SNPs and short indels) analyzing genotype data from a RIL population at F7 generation derived from the interspecific cross between L. culinaris cv. Alpo and L. odemensis accession ILWL235. L. odemensis is a wild species included in the secondary gene pool and can be used as a source for gene introgression in lentil breeding programs. Marker data were used to construct the first genetic interspecific map between these two species. This linkage map has been used to precisely identify regions of the CDC-Redberry lentil draft genome in which the candidate genes for some qualitative traits (seed coat spotting pattern, flower color, and stem pigmentation) could be located. The genome regions corresponding to a significant single quantitative trait locus (QTL) controlling “time to flowering” located in chromosome 6 and three QTLs regulating seed size and positioned in chromosomes 1 and 5 (two QTLs) were also identified. Significant QTLs for Ascochyta blight resistance in lentil were mapped to chromosome 6 in the genome region or close to it where QTLs for Ascochyta blight resistance have previously been reported.

ACS Style

Carlos Polanco; Luis E. Sáenz de Miera; Ana Isabel González; Pedro García García; Richard Fratini; Francisca Vaquero; Francisco Javier Vences; Marcelino Pérez De La Vega. Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil. PLoS ONE 2019, 14, e0214409 .

AMA Style

Carlos Polanco, Luis E. Sáenz de Miera, Ana Isabel González, Pedro García García, Richard Fratini, Francisca Vaquero, Francisco Javier Vences, Marcelino Pérez De La Vega. Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil. PLoS ONE. 2019; 14 (3):e0214409.

Chicago/Turabian Style

Carlos Polanco; Luis E. Sáenz de Miera; Ana Isabel González; Pedro García García; Richard Fratini; Francisca Vaquero; Francisco Javier Vences; Marcelino Pérez De La Vega. 2019. "Construction of a high-density interspecific (Lens culinaris x L. odemensis) genetic map based on functional markers for mapping morphological and agronomical traits, and QTLs affecting resistance to Ascochyta in lentil." PLoS ONE 14, no. 3: e0214409.

Journal article
Published: 30 June 2017 in BMC Plant Biology
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Frost is one of the main abiotic stresses limiting plant distribution and crop production. To cope with the stress, plants evolved adaptations known as cold acclimation or chilling tolerance to maximize frost tolerance. Cold acclimation is a progressive acquisition of freezing tolerance by plants subjected to low non-freezing temperatures which subsequently allows them to survive exposure to frost. Lentil is a cool season grain legume that is challenged by winter frost in some areas of its cultivation. To better understand the genetic base of frost tolerance differential gene expression in response to cold acclimation was investigated. Recombinant inbred lines (RILs) from the cross Precoz x WA8649041 were first classified as cold tolerant or cold susceptible according to their response to temperatures between −3 to −15 °C. Then, RILs from both extremes of the response curve were cold acclimated and the leaf transcriptomes of two bulks each of eight frost tolerant and seven cold susceptible RILs were investigated by Deep Super-SAGE transcriptome profiling. Thus, four RNA bulks were analysed: the acclimated susceptible, the acclimated tolerant and the respective controls (non-acclimated susceptible and non-acclimated tolerant). Approximately 16.5 million 26 nucleotide long Super-SAGE tags were sequenced in the four sets (between ~3 and 5.4 millions). In total, 133,077 different unitags, each representing a particular transcript isoform, were identified in these four sets. Tags which showed a significantly different abundance in any of the bulks (fold change ≥4.0 and a significant p-value <0.001) were selected and used to identify the corresponding lentil gene sequence. Three hundred of such lentil sequences were identified. Most of their known homologs coded for glycine-rich, cold and drought-regulated proteins, dormancy-associated proteins, proline-rich proteins (PRPs) and other membrane proteins. These were generally but not exclusively over-expressed in the acclimated tolerant lines. This set of candidate genes implicated in the response to frost in lentil represents an useful base for deeper and more detailed investigations into this important agronomic trait in future.

ACS Style

Abel Barrios; Constantino Caminero; Pedro García; Nicolas Krezdorn; Klaus Hoffmeier; Peter Winter; Marcelino Pérez De La Vega. Deep Super-SAGE transcriptomic analysis of cold acclimation in lentil (Lens culinaris Medik.). BMC Plant Biology 2017, 17, 111 -111.

AMA Style

Abel Barrios, Constantino Caminero, Pedro García, Nicolas Krezdorn, Klaus Hoffmeier, Peter Winter, Marcelino Pérez De La Vega. Deep Super-SAGE transcriptomic analysis of cold acclimation in lentil (Lens culinaris Medik.). BMC Plant Biology. 2017; 17 (1):111-111.

Chicago/Turabian Style

Abel Barrios; Constantino Caminero; Pedro García; Nicolas Krezdorn; Klaus Hoffmeier; Peter Winter; Marcelino Pérez De La Vega. 2017. "Deep Super-SAGE transcriptomic analysis of cold acclimation in lentil (Lens culinaris Medik.)." BMC Plant Biology 17, no. 1: 111-111.

Research article
Published: 27 April 2017 in PLOS ONE
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Retrotransposons with long terminal repeats (LTR-RTs) are widespread mobile elements in eukaryotic genomes. We obtained a total of 81 partial LTR-RT sequences from lentil corresponding to internal retrotransposon components and LTRs. Sequences were obtained by PCR from genomic DNA. Approximately 37% of the LTR-RT internal sequences presented premature stop codons, pointing out that these elements must be non-autonomous. LTR sequences were obtained using the iPBS technique which amplifies sequences between LTR-RTs. A total of 193 retrotransposon-derived genetic markers, mainly iPBS, were used to obtain a genetic linkage map from 94 F7 inbred recombinant lines derived from the cross between the cultivar Lupa and the wild ancestor L. culinaris subsp. orientalis. The genetic map included 136 markers located in eight linkage groups. Clusters of tightly linked retrotransposon-derived markers were detected in linkage groups LG1, LG2, and LG6, hence denoting a non-random genomic distribution. Phylogenetic analyses identified the LTR-RT families in which internal and LTR sequences are included. Ty3-gypsy elements were more frequent than Ty1-copia, mainly due to the high Ogre element frequency in lentil, as also occurs in other species of the tribe Vicieae. LTR and internal sequences were used to analyze in silico their distribution among the contigs of the lentil draft genome. Up to 8.8% of the lentil contigs evidenced the presence of at least one LTR-RT similar sequence. A statistical analysis suggested a non-random distribution of these elements within of the lentil genome. In most cases (between 97% and 72%, depending on the LTR-RT type) none of the internal sequences flanked by the LTR sequence pair was detected, suggesting that defective and non-autonomous LTR-RTs are very frequent in lentil. Results support that LTR-RTs are abundant and widespread throughout of the lentil genome and that they are a suitable source of genetic markers useful to carry out further genetic analyses.

ACS Style

Rita Rey-Baños; Luis E. Sáenz De Miera; Pedro García García; Marcelino Perez de la Vega. Obtaining retrotransposon sequences, analysis of their genomic distribution and use of retrotransposon-derived genetic markers in lentil (Lens culinaris Medik.). PLOS ONE 2017, 12, e0176728 .

AMA Style

Rita Rey-Baños, Luis E. Sáenz De Miera, Pedro García García, Marcelino Perez de la Vega. Obtaining retrotransposon sequences, analysis of their genomic distribution and use of retrotransposon-derived genetic markers in lentil (Lens culinaris Medik.). PLOS ONE. 2017; 12 (4):e0176728.

Chicago/Turabian Style

Rita Rey-Baños; Luis E. Sáenz De Miera; Pedro García García; Marcelino Perez de la Vega. 2017. "Obtaining retrotransposon sequences, analysis of their genomic distribution and use of retrotransposon-derived genetic markers in lentil (Lens culinaris Medik.)." PLOS ONE 12, no. 4: e0176728.

Journal article
Published: 01 September 2016 in Biological Conservation
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Borja Jiménez-Alfaro; Laura García-Calvo; Pedro García García; José-Luis Acebes. Anticipating extinctions of glacial relict populations in mountain refugia. Biological Conservation 2016, 201, 243 -251.

AMA Style

Borja Jiménez-Alfaro, Laura García-Calvo, Pedro García García, José-Luis Acebes. Anticipating extinctions of glacial relict populations in mountain refugia. Biological Conservation. 2016; 201 ():243-251.

Chicago/Turabian Style

Borja Jiménez-Alfaro; Laura García-Calvo; Pedro García García; José-Luis Acebes. 2016. "Anticipating extinctions of glacial relict populations in mountain refugia." Biological Conservation 201, no. : 243-251.

Journal article
Published: 22 April 2016 in Euphytica
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Bacterial blight caused by Pseudomonas syringae pv. pisi is an important disease of pea (Pisum sativum L.). Knowledge about the genes or mechanisms acting against this pathogen is scarce. A genetic mapping study of the resistance was performed using a recombinant inbred line mapping population generated by crossing the susceptible cultivar Cheyenne to the resistant line ZP0104. Resistance to race 2 was a dominant monogenic trait mapped at 109.4 cM on LGVII between AB114 and AB122 simple sequence repeat markers. It is assumed to be the previously Ppi2 based on its location. Resistance to races 4 and 8 behaved also as dominant monogenic traits and presumably due to a single gene conferring resistance to both races. It was mapped on LGIII at 11.2 cM of the AD57 microsatellite marker. This gene has been named Ppi8 and it is proposed as a new resistance gene. Ppi8 and Ppi2 are associated to the pea consensus map for the first time. To identify genes related to resistance and susceptibility, a DeepSuperSAGE genome-wide transcription profiling approach was conducted producing 45,261 different pea unitags. A set of 651 unitags were differently represented in the susceptible versus the resistant response with a significance of P < 0.001 and a fold change >4. These putative differentially expressed set includes genes previously related to responses to biotic stresses (pathogenesis-related or related to disease resistance responses), abiotic stresses or of unknown function, among other.

ACS Style

Alberto Martín-Sanz; Trinidad Aparicio; José Carlos Santana; Pedro García; Peter Winter; Constantino Caminero; Marcelino Pérez De La Vega. Mapping genes for resistance to bacterial blight (Pseudomonas syringae pv. pisi) in pea and identification of genes involved in resistance by DeepsuperSAGE transcriptome profiling. Euphytica 2016, 210, 375 -392.

AMA Style

Alberto Martín-Sanz, Trinidad Aparicio, José Carlos Santana, Pedro García, Peter Winter, Constantino Caminero, Marcelino Pérez De La Vega. Mapping genes for resistance to bacterial blight (Pseudomonas syringae pv. pisi) in pea and identification of genes involved in resistance by DeepsuperSAGE transcriptome profiling. Euphytica. 2016; 210 (3):375-392.

Chicago/Turabian Style

Alberto Martín-Sanz; Trinidad Aparicio; José Carlos Santana; Pedro García; Peter Winter; Constantino Caminero; Marcelino Pérez De La Vega. 2016. "Mapping genes for resistance to bacterial blight (Pseudomonas syringae pv. pisi) in pea and identification of genes involved in resistance by DeepsuperSAGE transcriptome profiling." Euphytica 210, no. 3: 375-392.

Research article
Published: 04 March 2015 in Journal of Integrative Plant Biology
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Maize (Zea mays L.) suspension‐cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor. Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy. Cell wall compositional analysis indicated that the cellulose‐deficient cell walls showed an enhancement of highly branched and cross‐linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p‐coumaric acid, and the presence of a polymer that stained positive for phloroglucinol. In accordance with this, cellulose‐deficient cell walls showed a fivefold increase in Klason‐type lignin. Thioacidolysis/GC‐MS analysis of cellulose‐deficient cell walls indicated the presence of a lignin‐like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress‐related lignin that arose in response to short‐term DCB‐treatments. Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin. A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions. In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.

ACS Style

Hugo Mélida; Asier Largo-Gosens; Esther Novo-Uzal; Rogelio Santiago; Federico Pomar; Pedro García; Penélope García-Angulo; José-Luis Acebes; Jesús Álvarez; Antonio Encina. Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures. Journal of Integrative Plant Biology 2015, 57, 357 -372.

AMA Style

Hugo Mélida, Asier Largo-Gosens, Esther Novo-Uzal, Rogelio Santiago, Federico Pomar, Pedro García, Penélope García-Angulo, José-Luis Acebes, Jesús Álvarez, Antonio Encina. Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures. Journal of Integrative Plant Biology. 2015; 57 (4):357-372.

Chicago/Turabian Style

Hugo Mélida; Asier Largo-Gosens; Esther Novo-Uzal; Rogelio Santiago; Federico Pomar; Pedro García; Penélope García-Angulo; José-Luis Acebes; Jesús Álvarez; Antonio Encina. 2015. "Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures." Journal of Integrative Plant Biology 57, no. 4: 357-372.

Journal article
Published: 05 March 2013 in Spanish Journal of Agricultural Research
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A previous Lens genetic map was improved by adding 31 molecular genetic markers, reaching a total of 190 markers with undistorted segregation. Data were obtained from the segregational analysis of 113 F2 plants generated from a single hybrid of Lens culinaris ssp. culinaris × L. c. ssp. orientalis. The added markers are predominantly codominant (15 SSRs, five CAPSs, four presence-absence polymorphisms, three length polymorphisms, two RAPDs, and two SRAPs). At a LOD score of 3.0, the 190 markers were grouped into eight linkage groups (LG) covering 2,234.4 cM, with an average distance between markers of 12.28 cM. This linkage map has reduced the numbers of linkage groups from ten in the previous map to eight. Most of the added markers must be functional markers since primers were mostly designed to amplify transcribed sequences. Some of the amplicons were sequenced to test if they were functional markers. One of the sequences showed homology with the Pisum TFL1a gene, involved in the transition from vegetative to flowering stages. This lentil gene was located in the LG 1 thanks to the presence of a polymorphic microsatellite in the first intron of the gene. Since L. culinaris ssp. orientalis is the primary source of additional genetic variability for lentil, this improved map could help in the use of such variability in lentil breeding programs.

ACS Style

R. De La Puente; Pedro García García; Carlos Polanco; Marcelino Perez de la Vega. Short communication. An improved intersubspecific genetic map in Lens including functional markers. Spanish Journal of Agricultural Research 2013, 11, 132 .

AMA Style

R. De La Puente, Pedro García García, Carlos Polanco, Marcelino Perez de la Vega. Short communication. An improved intersubspecific genetic map in Lens including functional markers. Spanish Journal of Agricultural Research. 2013; 11 (1):132.

Chicago/Turabian Style

R. De La Puente; Pedro García García; Carlos Polanco; Marcelino Perez de la Vega. 2013. "Short communication. An improved intersubspecific genetic map in Lens including functional markers." Spanish Journal of Agricultural Research 11, no. 1: 132.

Book chapter
Published: 16 November 2009 in Crop wild relative conservation and use
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Most of the species described in Spain are abundantly present throughout Europe (i.e. A. barbata, A. sterilis and A. fatua), however, three of them...

ACS Style

Pedro García García; Luis E. Sáenz de Miera; F. J. Vences; M. Benchacho; Marcelino Perez de la Vega. Conservation of Spanish wild oats: Avena canariensis, A. prostrata and A. murphyi. Crop wild relative conservation and use 2009, 413 -428.

AMA Style

Pedro García García, Luis E. Sáenz de Miera, F. J. Vences, M. Benchacho, Marcelino Perez de la Vega. Conservation of Spanish wild oats: Avena canariensis, A. prostrata and A. murphyi. Crop wild relative conservation and use. 2009; ():413-428.

Chicago/Turabian Style

Pedro García García; Luis E. Sáenz de Miera; F. J. Vences; M. Benchacho; Marcelino Perez de la Vega. 2009. "Conservation of Spanish wild oats: Avena canariensis, A. prostrata and A. murphyi." Crop wild relative conservation and use , no. : 413-428.

Journal article
Published: 01 September 2007 in Spanish Journal of Agricultural Research
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Lentil quantitative trait loci (QTL) related to plant structure (branches at first node, height of first node, total number of branches, plant height), growth habit (flowering time, pod dehiscence) and yield (number of seeds, seed weight, seed diameter) were located using a F2 population of 113 individuals derived from the intersubspecific cross of Lens culinaris ssp. culinaris and L. c. ssp. orientalis. Several traits were found to be significantly correlated. Using interval and composite interval mapping a total of 23 QTL for nine quantitative traits were located. No QTL was identified for the number of F3 seed produced. Six QTL were positioned respectively in linkage groups III and VI, and five QTL in linkage group I. Each remaining group included one or two QTL, except groups VII and IX where no QTL was found. The multiple QTL model explained more than 80% of the observed phenotypic variance with logarithm of the odds (LOD) scores above 10 for three of the quantitative traits analyzed (branches at first node, flowering time, and dehiscence). For the remaining traits the phenotypic variance explained was relatively low, between the 50% and 20%, and the LOD scores ranged between 4 and 8. The possible homology between some QTL and other previously described is discussed in relation to their chromosomal location.

ACS Style

Richard Mario Fratini; Y. Durán; Pedro García García; Marcelino Perez de la Vega. Identification of quantitative trait loci (QTL) for plant structure, growth habit and yield in lentil. Spanish Journal of Agricultural Research 2007, 5, 348 .

AMA Style

Richard Mario Fratini, Y. Durán, Pedro García García, Marcelino Perez de la Vega. Identification of quantitative trait loci (QTL) for plant structure, growth habit and yield in lentil. Spanish Journal of Agricultural Research. 2007; 5 (3):348.

Chicago/Turabian Style

Richard Mario Fratini; Y. Durán; Pedro García García; Marcelino Perez de la Vega. 2007. "Identification of quantitative trait loci (QTL) for plant structure, growth habit and yield in lentil." Spanish Journal of Agricultural Research 5, no. 3: 348.

Journal article
Published: 01 October 2006 in Plant Breeding
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Richard Mario Fratini; P. Garcia; M. L. Ruiz. Pollen and pistil morphology, in vitro pollen grain germination and crossing success of Lens cultivars and species. Plant Breeding 2006, 125, 501 -505.

AMA Style

Richard Mario Fratini, P. Garcia, M. L. Ruiz. Pollen and pistil morphology, in vitro pollen grain germination and crossing success of Lens cultivars and species. Plant Breeding. 2006; 125 (5):501-505.

Chicago/Turabian Style

Richard Mario Fratini; P. Garcia; M. L. Ruiz. 2006. "Pollen and pistil morphology, in vitro pollen grain germination and crossing success of Lens cultivars and species." Plant Breeding 125, no. 5: 501-505.

Journal article
Published: 12 January 2006 in Genetic Resources and Crop Evolution
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Genetic diversity was analysed in 52 Argentinian populations of Avena barbata, a tetraploid grass introduced in America from Spain during the colonization period. Nine isozyme systems were studied and 14 loci identified, five of which were polymorphic. Cluster analysis based on Hedrick’s index revealed a high similarity among populations. The total diversity (P t ) in the 52 populations was 0.144, the mean diversity (P s ) was 0.04, while between population diversity (D st ) was 0.103. The resulting coefficient of differentiation (G st ) was 0.714, indicating that diversity among populations was an important contributor to the total variability. Genetic diversity was structured into multilocus associations; 122 different complexes were found among 3311 individuals, but only two complexes occurred at a high frequency. The distribution pattern of these frequent multilocus genotypes was associated with environmental factors, mainly rainfall and temperature. The comparison of these results with those of previous studies on A. barbata from Spain indicated that Spanish and Argentinian populations are closely similar in allelic composition on a locus-by-locus basis but different in multilocus genotypic composition. We concluded that selection was the main force involved in the reorganization of the Spanish genepool into novel multilocus associations adapted to specific habitats in Argentina.

ACS Style

Irma-Rosana Guma; Marcelino Pérez De La Vega; Pedro García. Isozyme Variation and Genetic Structure of Populations of Avena barbata from Argentina. Genetic Resources and Crop Evolution 2006, 53, 587 -601.

AMA Style

Irma-Rosana Guma, Marcelino Pérez De La Vega, Pedro García. Isozyme Variation and Genetic Structure of Populations of Avena barbata from Argentina. Genetic Resources and Crop Evolution. 2006; 53 (3):587-601.

Chicago/Turabian Style

Irma-Rosana Guma; Marcelino Pérez De La Vega; Pedro García. 2006. "Isozyme Variation and Genetic Structure of Populations of Avena barbata from Argentina." Genetic Resources and Crop Evolution 53, no. 3: 587-601.

Comparative study
Published: 16 December 2003 in Theoretical and Applied Genetics
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A Lens map was developed based on the segregational analysis of five kinds of molecular and morphological genetic markers in 113 F2 plants obtained from a single hybrid of Lens culinaris ssp. culinaris × L. c. ssp. orientalis. A total of 200 markers were used on the F2 population, including 71 RAPDs, 39 ISSRs, 83 AFLPs, two SSRs and five morphological loci. The AFLP technique generated more polymorphic markers than any of the others, although AFLP markers also showed the highest proportion (29.1%) of distorted segregation. At a LOD score of 3.0, 161 markers were grouped into ten linkage groups covering 2,172.4 cM, with an average distance between markers of 15.87 cM. There were six large groups with 12 or more markers each, and four small groups with two or three markers each. Thirty-nine markers were unlinked. A tendency for markers to cluster in the central regions of large linkage groups was observed. Likewise, clusters of AFLP, ISSR or RAPD markers were also observed in some linkage groups, although RAPD markers were more evenly spaced along the linkage groups. In addition, two SSR, three RAPD and one ISSR markers segregated as codominant. ISSR markers are valuable tools for Lens genetic mapping and they have a high potential in the generation of saturated Lens maps.

ACS Style

Y. Durán; Richard Mario Fratini; Pedro García García; M. Pérez De La Vega. An intersubspecific genetic map of Lens. Theoretical and Applied Genetics 2003, 108, 1265 -1273.

AMA Style

Y. Durán, Richard Mario Fratini, Pedro García García, M. Pérez De La Vega. An intersubspecific genetic map of Lens. Theoretical and Applied Genetics. 2003; 108 (7):1265-1273.

Chicago/Turabian Style

Y. Durán; Richard Mario Fratini; Pedro García García; M. Pérez De La Vega. 2003. "An intersubspecific genetic map of Lens." Theoretical and Applied Genetics 108, no. 7: 1265-1273.

Comparative study
Published: 01 January 2002 in Cellular & Molecular Biology Letters
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Mohammed Benchacho; Rosana Guma; Marcelino Pérez De La Vega; Pedro García García. The genetic structure of tetraploid Avena: a comparison of isozyme and RAPD markers. Cellular & Molecular Biology Letters 2002, 7, 1 .

AMA Style

Mohammed Benchacho, Rosana Guma, Marcelino Pérez De La Vega, Pedro García García. The genetic structure of tetraploid Avena: a comparison of isozyme and RAPD markers. Cellular & Molecular Biology Letters. 2002; 7 (2):1.

Chicago/Turabian Style

Mohammed Benchacho; Rosana Guma; Marcelino Pérez De La Vega; Pedro García García. 2002. "The genetic structure of tetraploid Avena: a comparison of isozyme and RAPD markers." Cellular & Molecular Biology Letters 7, no. 2: 1.

Journal article
Published: 01 January 2002 in Genetic Resources and Crop Evolution
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The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of 43 multilocus associations per accession. In general, genetic variation within accessions was higher than between accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation of genetic variation in plant genetic resource collections.

ACS Style

El-Habib Dakir; Maria-Luisa Ruiz; Pedro García; Marcelino Pérez De La Vega. Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs. Genetic Resources and Crop Evolution 2002, 49, 619 -631.

AMA Style

El-Habib Dakir, Maria-Luisa Ruiz, Pedro García, Marcelino Pérez De La Vega. Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs. Genetic Resources and Crop Evolution. 2002; 49 (6):619-631.

Chicago/Turabian Style

El-Habib Dakir; Maria-Luisa Ruiz; Pedro García; Marcelino Pérez De La Vega. 2002. "Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs." Genetic Resources and Crop Evolution 49, no. 6: 619-631.

Journal article
Published: 01 March 1999 in Parasitology International
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2 figures, 2 tables.-- PMID: 11269322 [PubMed].Genetic variability of adult specimens of Dicrocoelium dendriticum has been studied using random amplified polymorphic DNA (RAPD). The worms were collected from the infected livers of different sheep from several localities in León province (NW Spain). DNA fragments were amplified by means of decamer primer oligonucleotides of arbitrary sequence. Some primers produce complex and highly variable patterns of amplified DNA in D. dendriticum. Intra- and inter-population genetic variability of adult parasites were analyzed, scoring polymorphic and monomorphic reproducible bands by means of the Jaccard similarity, and dendrograms showing genetic relationships between individuals were obtained using the FITCH method. Genetic variability seems to be high in this parasite and genetic similarity within a population (worms infecting a single animal) is similar to the average similarity between worms from different sheep. These results suggest that each sheep is infected by numerous genetically different parasites from one or more populations of infected ants.This study was supported by the Spanish CICYT, Projects number AGF92-0588 and AGF96-0416.Peer reviewe

ACS Style

Hilda Sandoval; M. Yolanda Manga-González; Raquel Campo; Pedro García García; Jose Maria Castro; Marcelino Pérez De La Vega. Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA. Parasitology International 1999, 48, 21 -26.

AMA Style

Hilda Sandoval, M. Yolanda Manga-González, Raquel Campo, Pedro García García, Jose Maria Castro, Marcelino Pérez De La Vega. Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA. Parasitology International. 1999; 48 (1):21-26.

Chicago/Turabian Style

Hilda Sandoval; M. Yolanda Manga-González; Raquel Campo; Pedro García García; Jose Maria Castro; Marcelino Pérez De La Vega. 1999. "Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA." Parasitology International 48, no. 1: 21-26.