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Wenxiong Lin
College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou, China

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Journal article
Published: 30 July 2021 in Agronomy
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Arsenic is one of the most dangerous metalloids, and silicon is a helpful element supporting plants to withstand stress. In this study, three factors were considered, including rice accessions with three different lines, including Lsi1-RNAi line (LE-R), Lsi1 overexpression line (LE-OE), and their wild type (LE-WT), and silicon and arsenic treatments with two different levels. Analysis of variance in dry weight biomass, protein content, arsenic, and silicon concentration has shown a significant interaction between three factors. Further analysis showed that the silicon concentration of all rice seedlings under silicon treatments increased significantly. The LE-OE line has shown a higher ability to absorb silicon in hydroponic conditions than the wild type, and when the seedlings were exposed to arsenic, the concentration of arsenic in all lines increased significantly. Adding silicon to over-expressed rice lines with the Lsi1 gene creates better arsenic resistance than their wild type. These findings confirmed antagonism between silicon and arsenic, and seedlings exposed to arsenic showed a reduction in silicon concentration in all rice lines. RNA-seq analysis showed 106 differentially expressed genes in the LE-OE line, including 75 up-regulated genes and 31 down-regulated genes. DEGs in the LE-R line were 449 genes, including 190 up-regulated and 259 down-regulated genes. Adding treatment has changed the expression of Calcium-binding EGF domain-containing, Os10g0530500, Os05g0240200 in both LE-OE and LE-R roots. They showed that transgenic cultivars were more resistant to arsenic than wild-type, especially when silicon was added to the culture medium.

ACS Style

Mohammad Boorboori; Wenxiong Lin; Yanyang Jiao; Changxun Fang. Silicon Modulates Molecular and Physiological Activities in Lsi1 Transgenic and Wild Lemont Rice Seedlings under Arsenic Stress. Agronomy 2021, 11, 1532 .

AMA Style

Mohammad Boorboori, Wenxiong Lin, Yanyang Jiao, Changxun Fang. Silicon Modulates Molecular and Physiological Activities in Lsi1 Transgenic and Wild Lemont Rice Seedlings under Arsenic Stress. Agronomy. 2021; 11 (8):1532.

Chicago/Turabian Style

Mohammad Boorboori; Wenxiong Lin; Yanyang Jiao; Changxun Fang. 2021. "Silicon Modulates Molecular and Physiological Activities in Lsi1 Transgenic and Wild Lemont Rice Seedlings under Arsenic Stress." Agronomy 11, no. 8: 1532.

Accepted manuscript
Published: 23 July 2021 in Journal of Experimental Botany
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Root-pathogen interactions influence premature senescence in rice, however, few studies have addressed the underlying mechanism. In this study, when premature senescence significantly occurred in the osvha-a1 mutant (loss of tonoplast H+-ATPase activity), the relative abundance of rhizospheric bacterial communities was similar between the mutant and its wild type, while the fungi in the rhizosphere of the osvha-a1 mutant significantly differed from the wild type. Furthermore, one key fungal strain in the rhizospheric soil of the osvha-a1 mutant, Gibberella intermedia, increased substantially during the late growing phase, resulting in severe accumulation of reactive oxygen species (ROS). By contrast, the wild type showed much lower levels of ROS when infected by G. intermedia. Using high performance liquid chromatography, sugars in root exudates were identified to be different between osvha-a1 mutant and the wild type. G. intermedia could use mannose and rhamnose in root exudates from the mutant more efficiently than any other sugar. Finally, antagonistic bacteria could be employed for limiting the proliferation of G. intermedia in the rhizosphere, thereby alleviating the early senescent phenotypes of the osvha-a1 mutant, and improving grain yield.

ACS Style

Feifan Lin; Puleng Letuma; Zhaowei Li; Sheng Lin; Christopher Rensing; Wenxiong Lin. Rhizospheric pathogen proliferation and ROS production is associated with premature senescence of the osvha-a1 rice mutant. Journal of Experimental Botany 2021, 1 .

AMA Style

Feifan Lin, Puleng Letuma, Zhaowei Li, Sheng Lin, Christopher Rensing, Wenxiong Lin. Rhizospheric pathogen proliferation and ROS production is associated with premature senescence of the osvha-a1 rice mutant. Journal of Experimental Botany. 2021; ():1.

Chicago/Turabian Style

Feifan Lin; Puleng Letuma; Zhaowei Li; Sheng Lin; Christopher Rensing; Wenxiong Lin. 2021. "Rhizospheric pathogen proliferation and ROS production is associated with premature senescence of the osvha-a1 rice mutant." Journal of Experimental Botany , no. : 1.

Journal article
Published: 09 July 2021 in Genomics
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Heat shock transcription factors (HSFs) can regulate plant development and stress response. The comprehensive evolutionary history of the HSF family remains elusive in cotton. In this study, each cotton species had 78 members in Gossypium barbadense and Gossypium hirsutum. The diploid species had 39 GaHSFs in Gossypium arboreum, 31 GrHSFs in Gossypium raimondii, 34 GtHSFs in Gossypium turneri, and 34 GlHSFs in Gossypium longicalyx. The HSF family in cotton can be classified into three subfamilies, with seven groups in subfamily A and five groups in subfamily B. Different groups exhibited distinct gene proportions, conserved motifs, gene structures, expansion rates, gene loss rates, and cis-regulatory elements. The paleohexaploidization event led to the expansion of the HSF family in cotton, and the gene duplication events in six Gossypium species were inherited from their common ancestor. The HSF family in diploid species had a divergent evolutionary history, whereas two cultivated tetraploids presented a highly conserved evolution of the HSF family. The HSF members in At and Dt subgenomes of the cultivated tetraploids showed a different evolution from their corresponding diploid donors. Some HSF members were regarded as key candidates for regulating cotton development and stress response. This study provided the comprehensive information on the evolutionary history of the HSF family in cotton.

ACS Style

Kai Fan; Zhijun Mao; Fangting Ye; Xinfeng Pan; Zhaowei Li; Weiwei Lin; Yongqiang Zhang; Jinwen Huang; Wenxiong Lin. Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species. Genomics 2021, 113, 3112 -3127.

AMA Style

Kai Fan, Zhijun Mao, Fangting Ye, Xinfeng Pan, Zhaowei Li, Weiwei Lin, Yongqiang Zhang, Jinwen Huang, Wenxiong Lin. Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species. Genomics. 2021; 113 (5):3112-3127.

Chicago/Turabian Style

Kai Fan; Zhijun Mao; Fangting Ye; Xinfeng Pan; Zhaowei Li; Weiwei Lin; Yongqiang Zhang; Jinwen Huang; Wenxiong Lin. 2021. "Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species." Genomics 113, no. 5: 3112-3127.

Research article
Published: 26 April 2021 in Environmental Science and Pollution Research
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Sugarcane monoculture (SM) often leads to soil problems, like soil acidification, degradation, and soil-borne diseases, which ultimately pose a negative impact on agricultural productivity and sustainability. Understanding the change in microbial communities’ composition, activities, and functional microbial taxa associated with the plant and soil under SM is unclear. Using multidisciplinary approaches such as Illumina sequencing, measurements of soil properties, and enzyme activities, we analyzed soil samples from three sugarcane fields with different monoculture histories (1-, 2-, and 4-year cultivation times, respectively). We observed that SM induced soil acidity and had adverse effects on soil fertility, i.e., soil organic matter (OM), total nitrogen (TN), total carbon (TC), and available potassium (AK), as well as enzyme activities indicative for carbon, phosphorus, and nitrogen cycles. Non-metric multidimensional scaling (NMDS) analysis showed that SM time greatly affected soil attribute patterns. We observed strong correlation among soil enzymes activities and soil physiochemical properties (soil pH, OM, and TC). Alpha diversity analysis showed a varying response of the microbes to SM time. Bacterial diversity increased with increasing oligotrophs (e.g., Acidobacteria and Chloroflexi), while fungal diversity decreased with reducing copiotrophs (e.g., Ascomycota). β-Diversity analysis showed that SM time had a great influence on soil microbial structure and soil properties, which led to the changes in major components of microbial structure (soil pH, OM, TC, bacteria and soil pH; TC, fungi). Additionally, SM time significantly stimulated (four bacterial and ten fungal) and depleted (12 bacterial and three fungal) agriculturally and ecologically important microbial genera that were strongly and considerably correlated with soil characteristics (soil pH, OM, TC, and AK). In conclusion, SM induces soil acidity, reduces soil fertility, shifts microbial structure, and reduces its activity. Furthermore, most beneficial bacterial genera decreased significantly due to SM, while beneficial fungal genera showed a reverse trend. Therefore, mitigating soil acidity, improving soil fertility, and soil enzymatic activities, including improved microbial structure with beneficial service to plants and soil, can be an effective measure to develop a sustainable sugarcane cropping system.

ACS Style

Muhammad Tayyab; Ziqi Yang; Caifang Zhang; Waqar Islam; Wenxiong Lin; Hua Zhang. Sugarcane monoculture drives microbial community composition, activity and abundance of agricultural-related microorganisms. Environmental Science and Pollution Research 2021, 1 -17.

AMA Style

Muhammad Tayyab, Ziqi Yang, Caifang Zhang, Waqar Islam, Wenxiong Lin, Hua Zhang. Sugarcane monoculture drives microbial community composition, activity and abundance of agricultural-related microorganisms. Environmental Science and Pollution Research. 2021; ():1-17.

Chicago/Turabian Style

Muhammad Tayyab; Ziqi Yang; Caifang Zhang; Waqar Islam; Wenxiong Lin; Hua Zhang. 2021. "Sugarcane monoculture drives microbial community composition, activity and abundance of agricultural-related microorganisms." Environmental Science and Pollution Research , no. : 1-17.

Research article
Published: 01 April 2021 in Plant Disease
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Radix pseudostellariae L. is one of the most common and highly-prized Chinese medicinal plants with various pharmacological effects, and mainly produced in acid soils in the Guizhou and Fujian provinces of southwestern and southeastern China, respectively (Wu et al. 2020). However, consecutive monoculture of R. pseudostellariae results in severe root rot and decline in biomass and quality of underground tubers. Root tubers of R. pseudostellariae are typically planted in December and harvested in next June. Root rot commonly starts developing in May. The disease incidence of root rot was ranging from 37 to 46% in root portions and basal stem of R. pseudostellariae under the consecutive monoculture fields in Shibing County, Guizhou Province, China (108°12ʹE, 27°03ʹN) (Li et al. 2017). Severe root rot was observed in Shibing County in May 2018. Infected plants displayed curly, withered, and yellow leaves, blight, retarded growth, root rot, and yield losses. Abundant whitish mycelia were observed on roots and surrounding soil. Two fungal isolates, designated GZ20190123 and GZ20190124, were obtained from symptomatic roots cultured on potato dextrose agar (PDA). The optimum temperature range for growth of the two isolates was 25 to 30°C. The optimum pH range for the growth of GZ20190123 was 5 to 5.5, whereas GZ20190124 grew better between pH 5 to 8.5. The mean mycelial growth rates of GZ20190123 and GZ20190124 at 30°C were 2.1 and 1.5 cm/day, respectively. Conidia of the two isolates were ovoid or obclavate and were produced in single or branched chains. The internal transcribed spacer (ITS) region was amplified with primers ITS1 and ITS4 (White et al. 1990). The sequences were deposited in GenBank as accession No. MN726736 for GZ20190123 and MN726738 for GZ20190124. Sequence comparison revealed 99% (GZ20190123) and 97% (GZ20190124) identity with previously reported isolate xsd08071 of Mucor racemosus Bull. (accession No. FJ582639.1) and isolate BM3 of Mucor fragilis Bainier (accession No. MK910058.1), respectively, which was confirmed by phylogenetic analysis. The two isolates were tested for pathogenicity on R. pseudostellariae. Six roots of R. pseudostellariae were surface-sterilized with 75% ethanol and stab inoculated with mycelia using a sterile toothpick for each isolate. Sterile distilled water was stab inoculated to twelve roots to serve as the control. Treated roots were incubated in a greenhouse with 16 h day length [light intensity 146.5 μmol/(m2·s)] and day/night temperature 26°C/18°C. The inoculated roots showed the expected symptoms on roots and sprouts 7 days after inoculation, whereas the control roots with sprouts did not show any symptom. The fungi were re-isolated from the diseased roots and confirmed as expected M. racemosus or M. fragilis based on the ITS sequences, which satisfied Koch’s postulates. Thus, isolate GZ20190123 was identified as M. racemosus and GZ20190124 as M. fragilis. Previously, M. racemosus and M. fragilis have been reported as a pathogen on tomato (Kwon and Hong 2005) and grape (Ghuffar et al. 2018), respectively. To our knowledge, this is the first report of M. racemosus and M. fragilis causing root rot of R. pseudostellariae in southwestern China, where the disease could cause a significant loss to production of this important medicinal plant.

ACS Style

Hongmiao Wu; Jiachun Wu; Feng Li; Ling Zheng; Jingkai Fan; Wenxiong Lin. First Report of Mucor spp. Causing Root Rot of Radix pseudostellariae in Guizhou Province of China. Plant Disease 2021, 105, 1200 .

AMA Style

Hongmiao Wu, Jiachun Wu, Feng Li, Ling Zheng, Jingkai Fan, Wenxiong Lin. First Report of Mucor spp. Causing Root Rot of Radix pseudostellariae in Guizhou Province of China. Plant Disease. 2021; 105 (4):1200.

Chicago/Turabian Style

Hongmiao Wu; Jiachun Wu; Feng Li; Ling Zheng; Jingkai Fan; Wenxiong Lin. 2021. "First Report of Mucor spp. Causing Root Rot of Radix pseudostellariae in Guizhou Province of China." Plant Disease 105, no. 4: 1200.

Journal article
Published: 16 March 2021 in Protein Expression and Purification
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The poor grain filling of inferior spikelets (IS) situated on the lower secondary rachis branch leads to a remarkable decrease in rice yield and quality. The AGPase small subunit 2 (AGPS2) encodes a small subunit of adenosine diphosphate-glucose pyrophosphorylase (AGPase) enzyme, which plays an important role in sucrose-starch conversion and starch biosynthesis in the grain filling of rice. In the present study, qPCR analysis showed low expression abundance of AGPS2 in IS, compared to the superior spikelets (SS), which was consistent with the lower grain weight of IS. To evaluate the molecular mechanism of AGPS2, we first identified the AGPS2 interaction network through Co-immunoprecipitation (Co-IP). In total, 29 proteins of AGPS2 interaction network were characterized by LC–MS/MS. Bioinformatics analysis revealed that, the characterized proteins in the interaction network are likely to be related to starch synthesis, sugar conversion, energy pathway, and folding/modification, and most of them were involved in the grain filling of rice. The sequent Co-IP analysis showed that AGPS2 can bind to starch branching enzyme (SBE), pullulanase (PUL) and starch debranching enzyme (DBE) and assemble into starch synthesizing protein complex (SSPC). In addition, the 14-3-3 protein GF14e was also found to interact with AGPS2. Further analysis by qPCR showed that the expression of GF14e was much higher on IS than on SS. The qPCR results also showed that the expression of GF14e was relatively stable in SS, but changed significantly in IS under alternate wetting and moderate soil drying (WMD), which is consistent with the AGPS2 expression pattern. Our present work provides direct molecular evidence for the different expression patterns of AGPS2 in SS and IS, which could be greatly helpful for the molecular amelioration of the poor grain filling of IS in rice.

ACS Style

Hong Zhao; Zhou Li; Hira Amjad; Guopei Zhong; Muhammad Umar Khan; Zhixing Zhang; Wenxiong Lin. Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets. Protein Expression and Purification 2021, 183, 105875 .

AMA Style

Hong Zhao, Zhou Li, Hira Amjad, Guopei Zhong, Muhammad Umar Khan, Zhixing Zhang, Wenxiong Lin. Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets. Protein Expression and Purification. 2021; 183 ():105875.

Chicago/Turabian Style

Hong Zhao; Zhou Li; Hira Amjad; Guopei Zhong; Muhammad Umar Khan; Zhixing Zhang; Wenxiong Lin. 2021. "Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets." Protein Expression and Purification 183, no. : 105875.

Short communication
Published: 16 January 2021 in Agriculture, Ecosystems & Environment
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Replanting disease is a complex system of stressors affecting multiple biological processes, consequently shaping the soil microbial community structure. However, only a few studies have focused on the fauna of the rhizosphere soil. In addition, the effects of replanting disease on the diversity of the faunal community are poorly understood. We collected samples of Radix pseudostellariae rhizosphere soils in different consecutive monoculture fields from three geo-authentic production zones. The microbial abundance and soil faunal community diversity were analyzed using qRT-PCR and 18S rRNA gene sequencing, respectively. Continuous monoculture significantly increased the diversity of the soil nematodes. A total of 14 faunal phyla were detected among all samples, which predominantly included Nematoda, Chordata, Annelida, Arthropoda, Platyhelminthes, Gastrotricha, and Apicomplexa. Compared to the 1-year monoculture, continuous monoculture significantly increased the relative abundances of plant parasites, plant pathogens, phototrophs, and parasites, while significantly reducing the relative abundance of omnivores. Co-occurrence network analysis indicated that continuous monoculture increased the connections and positive correlations of the nematode community. Spearman correlation analysis showed that the soil faunal taxa were significantly associated with various soil factors (pH, sucrase, NO3−-N, NH4+-N, total bacterial, and fungal abundance). Our findings have extended our knowledge regarding the effect of replanting disease on the functional composition of rhizosphere fauna, and the results highlight the role of continuous monoculture in shaping faunal community diversity.

ACS Style

Hongmiao Wu; Huiming Wu; Xianjin Qin; Manhong Lin; Yanlin Zhao; Christopher Rensing; Wenxiong Lin. Replanting disease alters the faunal community composition and diversity in the rhizosphere soil of Radix pseudostellariae. Agriculture, Ecosystems & Environment 2021, 310, 107304 .

AMA Style

Hongmiao Wu, Huiming Wu, Xianjin Qin, Manhong Lin, Yanlin Zhao, Christopher Rensing, Wenxiong Lin. Replanting disease alters the faunal community composition and diversity in the rhizosphere soil of Radix pseudostellariae. Agriculture, Ecosystems & Environment. 2021; 310 ():107304.

Chicago/Turabian Style

Hongmiao Wu; Huiming Wu; Xianjin Qin; Manhong Lin; Yanlin Zhao; Christopher Rensing; Wenxiong Lin. 2021. "Replanting disease alters the faunal community composition and diversity in the rhizosphere soil of Radix pseudostellariae." Agriculture, Ecosystems & Environment 310, no. : 107304.

Journal article
Published: 29 September 2020 in Field Crops Research
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The grain quality of rice in the ratoon cultivation system is often higher than in the main crop (late season). To investigate the reason for that difference, a study of physiochemical factors, particularly relevant enzyme activities and their relationships with grain-filling and rice quality, was conducted with two hybrid rice cultivars, indica type "Taifengyou 3301″ and indica-japonica type "Yongyou 2640″. The results showed that the head rice yield of the superior and inferior grains in ratoon rice were significantly higher than those of their counterpart main crops (late season) to which rice heading time was synchronized. The degree of grain chalkiness and chalky grain percentage of both superior and inferior grains in ratoon rice were much lower than those of the late season main crop of both cultivars over two years of study. The starch RVA (Rapid Viscosity Analyzer) profile parameter analysis indicated that the maximum viscosity and setback viscosity of the superior and inferior grains of ratoon rice were higher than the late season main crop counterparts in both cultivars. We also found that the grain-filling of ratoon rice, especially for inferior grains, initiated earlier and continued longer and steadier than that of the late season main crop at the early and middle stages, which was driven by better translocation of photosynthate and the coordinated activity of enzymes including starch synthase and starch branching enzyme. These findings suggest that lower grain sink capacity and higher photosynthate remobilization ability result in fast and steady grain-filling in ratoon crops. Furthermore, coordinated enzyme activity appears to be involved in the process of starchsynthesis and dry matter translocation that drives the improvement of grain-filling and the grain quality of ratoon rice.

ACS Style

Jinwen Huang; Yiping Pan; Hongfei Chen; Zhixing Zhang; Changxun Fang; Caihong Shao; Hira Amjad; Weiwei Lin; Wenxiong Lin. Physiochemical mechanisms involved in the improvement of grain-filling, rice quality mediated by related enzyme activities in the ratoon cultivation system. Field Crops Research 2020, 258, 107962 .

AMA Style

Jinwen Huang, Yiping Pan, Hongfei Chen, Zhixing Zhang, Changxun Fang, Caihong Shao, Hira Amjad, Weiwei Lin, Wenxiong Lin. Physiochemical mechanisms involved in the improvement of grain-filling, rice quality mediated by related enzyme activities in the ratoon cultivation system. Field Crops Research. 2020; 258 ():107962.

Chicago/Turabian Style

Jinwen Huang; Yiping Pan; Hongfei Chen; Zhixing Zhang; Changxun Fang; Caihong Shao; Hira Amjad; Weiwei Lin; Wenxiong Lin. 2020. "Physiochemical mechanisms involved in the improvement of grain-filling, rice quality mediated by related enzyme activities in the ratoon cultivation system." Field Crops Research 258, no. : 107962.

Regular article
Published: 10 August 2020 in Plant and Soil
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Traditional agricultural systems increasingly contribute to food and livelihood security worldwide. Previous studies have demonstrated the positive effects of intercropping on crop productivity due to the reduced impact of disease. However, surprisingly few studies to date have explored the mechanisms underlying the alleviation of agricultural replanting disease by intraspecific intercropping. Here, we performed metabolomic, high-throughput sequencing and quantitative PCR analysis to explore the beneficial effects of intraspecific intercropping on Radix pseudostellariae yields, soil metabolite abundance and microbial communities. Our results showed that intraspecific intercropping alleviated the serious replanting disease of R. pseudostellariae. The intercropping increased fungal and bacterial community diversity, increasing the relative abundances of potentially beneficial taxa (e.g., Nitrosomonadales, Nitrospirales, and Pseudomonadales), and decreasing the relative abundances of pathogenic taxa (e.g., Aspergillus and Talaromyces). Quantitative PCR analysis showed intercropping significantly decreased the populations of pathogenic F. oxysporum, and increased the beneficial Pseudomonas spp. and Burkholderia spp. as compared to monoculture. Our findings also suggested that intraspecific intercropping tightened associations within the soil microbiome and promoted microbial co-occurrence. Specifically, we observed that soils subjected to the consecutive monoculture of two plant varieties contained fewer metabolites, while intercropping increased the abundances of metabolites in the second cropping year. The metabolites most reduced after intercropping were negatively correlated with the beneficial taxa Bacillus, Pseudomonas, Nitrobacter, and Penicillium. Thus, intercropping drove changes in root exudate metabolites, which subsequently shaped the rhizosphere microbiome by increasing microbial diversity. This consequently improved soil nutrients and reduced disease in the consecutive monocultures.

ACS Style

Hongmiao Wu; Manhong Lin; Christopher Rensing; Xianjin Qin; Shengkai Zhang; Jun Chen; Linkun Wu; Yanlin Zhao; Sheng Lin; Wenxiong Lin. Plant-mediated rhizospheric interactions in intraspecific intercropping alleviate the replanting disease of Radix pseudostellariae. Plant and Soil 2020, 454, 411 -430.

AMA Style

Hongmiao Wu, Manhong Lin, Christopher Rensing, Xianjin Qin, Shengkai Zhang, Jun Chen, Linkun Wu, Yanlin Zhao, Sheng Lin, Wenxiong Lin. Plant-mediated rhizospheric interactions in intraspecific intercropping alleviate the replanting disease of Radix pseudostellariae. Plant and Soil. 2020; 454 (1-2):411-430.

Chicago/Turabian Style

Hongmiao Wu; Manhong Lin; Christopher Rensing; Xianjin Qin; Shengkai Zhang; Jun Chen; Linkun Wu; Yanlin Zhao; Sheng Lin; Wenxiong Lin. 2020. "Plant-mediated rhizospheric interactions in intraspecific intercropping alleviate the replanting disease of Radix pseudostellariae." Plant and Soil 454, no. 1-2: 411-430.

Original research article
Published: 23 July 2020 in Frontiers in Microbiology
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The use of plant allelopathy to control weeds in the field has been generally recognized as a win-win strategy because it is an environmentally friendly and resource-saving method. The mechanism of this natural weed-control method relies on allelochemicals, the rhizosphere microbiome, and their bio-interaction, and exploring the link between allelochemicals and specific microbes helps accelerate the application of allelopathic characteristics in farming. In this study, we used allelopathic rice PI312777 (PI), its genetically modified OsPAL2-1 repression (PR) or overexpression (PO) lines, and non-allelopathic rice Lemont (Le) as donor plants to reveal the bio-interaction between rice allelochemicals and rhizosphere specific microorganisms. The results showed a higher content of phenolic acid exudation from the roots of PI than those of Le, which resulted in a significantly increased population of Myxococcus in the rhizosphere soil. Transgenic PO lines exhibited increasing exudation of phenolic acid, which led to the population of Myxococcus xanthus in the rhizosphere soil of PO to be significantly increased, while PR showed the opposite result in comparison with wild type PI. Exogenous application of phenolic acid induced the growth of M. xanthus, and the expressions of chemotaxis-related genes were up-regulated in M. xanthus. In addition, quercetin was identified in the culture medium; according to the bioassay determination, a quercetin concentration of 0.53 mM inhibited the root length by 60.59%. Our study indicates that OsPAL2-1 is among the efficient genes that regulate rice allelopathy by controlling the synthesis of phenolic acid allelochemicals, and phenolic acid (ferulic acid, FA) induces the chemotactic aggregation of M. xanthus, which promoted the proliferation and aggregation of this microbe. The potential allelochemical, quercetin was generated from the FA-induced M. xanthus cultured medium.

ACS Style

Yingzhe Li; Xin Jian; Yue Li; Xiaomei Zeng; Lining Xu; Muhammad Umar Khan; Wenxiong Lin. OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem. Frontiers in Microbiology 2020, 11, 1411 .

AMA Style

Yingzhe Li, Xin Jian, Yue Li, Xiaomei Zeng, Lining Xu, Muhammad Umar Khan, Wenxiong Lin. OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem. Frontiers in Microbiology. 2020; 11 ():1411.

Chicago/Turabian Style

Yingzhe Li; Xin Jian; Yue Li; Xiaomei Zeng; Lining Xu; Muhammad Umar Khan; Wenxiong Lin. 2020. "OsPAL2-1 Mediates Allelopathic Interactions Between Rice and Specific Microorganisms in the Rhizosphere Ecosystem." Frontiers in Microbiology 11, no. : 1411.

Journal article
Published: 06 July 2020 in BMC Genomics
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Soybean (Glycine max) is an important oil provider and ecosystem participant. The protein phosphatase 2C (PP2C) plays important roles in key biological processes. Molecular evolution and functional analysis of the PP2C family in soybean are yet to be reported. The present study identified 134 GmPP2Cs with 10 subfamilies in soybean. Duplication events were prominent in the GmPP2C family, and all duplicated gene pairs were involved in the segmental duplication events. The legume-common duplication event and soybean-specific tetraploid have primarily led to expanding GmPP2C members in soybean. Sub-functionalization was the main evolutionary fate of duplicated GmPP2C members. Meanwhile, massive genes were lost in the GmPP2C family, especially from the F subfamily. Compared with other genes, the evolutionary rates were slower in the GmPP2C family. The PP2C members from the H subfamily resembled their ancestral genes. In addition, some GmPP2Cs were identified as the putative key regulator that could control plant growth and development. A total of 134 GmPP2Cs were identified in soybean, and their expansion, molecular evolution and putative functions were comprehensively analyzed. Our findings provided the detailed information on the evolutionary history of the GmPP2C family, and the candidate genes can be used in soybean breeding.

ACS Style

Kai Fan; Yunrui Chen; Zhijun Mao; Yao Fang; Zhaowei Li; Weiwei Lin; Yongqiang Zhang; Jianping Liu; Jinwen Huang; Wenxiong Lin. Pervasive duplication, biased molecular evolution and comprehensive functional analysis of the PP2C family in Glycine max. BMC Genomics 2020, 21, 1 -17.

AMA Style

Kai Fan, Yunrui Chen, Zhijun Mao, Yao Fang, Zhaowei Li, Weiwei Lin, Yongqiang Zhang, Jianping Liu, Jinwen Huang, Wenxiong Lin. Pervasive duplication, biased molecular evolution and comprehensive functional analysis of the PP2C family in Glycine max. BMC Genomics. 2020; 21 (1):1-17.

Chicago/Turabian Style

Kai Fan; Yunrui Chen; Zhijun Mao; Yao Fang; Zhaowei Li; Weiwei Lin; Yongqiang Zhang; Jianping Liu; Jinwen Huang; Wenxiong Lin. 2020. "Pervasive duplication, biased molecular evolution and comprehensive functional analysis of the PP2C family in Glycine max." BMC Genomics 21, no. 1: 1-17.

Journal article
Published: 09 June 2020 in BMC Plant Biology
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Background Identification of the allelopathy-interrelated metabolites from the allelopathic rice rhizosphere is crucial to understand the allelopathic mechanism of rice, which in turn can promote its applications to farming. In this study, the metabolites from the rhizosphere soil of five different rice lines, including allelopathic rice accession PI312777 (PI) and non-allelopathic rice accession Lemont (Le) as well as their genetic derivatives (e.g., phenylalanine ammonia-lyase (PAL) gene overexpression transgenic lines of PI and Le, namely, PO and LO respectively, and PAL RNA interference line of PI, namely, PR) were identified and comparatively analyzed to explore the positive compounds that are involved in the process of rice allelopathy. Results The results showed that 21 non-polar compounds and 21 polar compounds differed in content in the rhizosphere soil of PI and Le, which include several volatile fatty acids and long-chain fatty acids. The relative contents of fatty acids also differed between PAL overexpressing or RNA interference (RNAi)-silenced line and their wild-type respectively. Acetic acid content also differed among groups, i.e., it is higher in the high allelopathic potential rice. Further analysis showed that different metabolites from the ADS8 resin-extracted phase were more abundant than that those from the ADS21 resin-extracted phase, suggesting that the allelochemicals in root exudates of allelopathic rice are mainly non-polar substances. KEGG annotation of these differential metabolites revealed that these compounds were related to nutrient metabolism, secondary metabolite synthesis, signaling substance synthesis, and toxin degradation. Conclusions Rice allelochemicals deposited in the ADS8 resin-extracted phase were more abundant than those in the ADS21 resin-extracted phase. Allelochemicals in root exudates of allelopathic rice are mainly non-polar substances, and long-chain fatty acids are considered as allelopathy interrelated metabolites.

ACS Style

Yingzhe Li; Lining Xu; Puleng Letuma; Wenxiong Lin. Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions. BMC Plant Biology 2020, 20, 1 -21.

AMA Style

Yingzhe Li, Lining Xu, Puleng Letuma, Wenxiong Lin. Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions. BMC Plant Biology. 2020; 20 (1):1-21.

Chicago/Turabian Style

Yingzhe Li; Lining Xu; Puleng Letuma; Wenxiong Lin. 2020. "Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions." BMC Plant Biology 20, no. 1: 1-21.

Journal article
Published: 26 May 2020 in BMC Plant Biology
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Background Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling. Results A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit α, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit β. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit β, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment. Conclusions Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit β is an intermediate junction between OsNIP2;1 and OsSHMT.

ACS Style

Changxun Fang; Pengli Zhang; Lanlan Li; Luke Yang; Dan Mu; Xue Yan; Zhong Li; Wenxiong Lin. Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. BMC Plant Biology 2020, 20, 1 -13.

AMA Style

Changxun Fang, Pengli Zhang, Lanlan Li, Luke Yang, Dan Mu, Xue Yan, Zhong Li, Wenxiong Lin. Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. BMC Plant Biology. 2020; 20 (1):1-13.

Chicago/Turabian Style

Changxun Fang; Pengli Zhang; Lanlan Li; Luke Yang; Dan Mu; Xue Yan; Zhong Li; Wenxiong Lin. 2020. "Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance." BMC Plant Biology 20, no. 1: 1-13.

Preprint content
Published: 19 May 2020
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Background: Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling. Results: A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit α, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit β. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit β, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment. Conclusions: Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit β is an intermediate junction between OsNIP2;1 and OsSHMT.

ACS Style

Changxun Fang; Pengli Zhang; Lanlan Li; Luke Yang; Dan Mu; Xue Yan; Zhong Li; Wenxiong Lin. Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. 2020, 1 .

AMA Style

Changxun Fang, Pengli Zhang, Lanlan Li, Luke Yang, Dan Mu, Xue Yan, Zhong Li, Wenxiong Lin. Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. . 2020; ():1.

Chicago/Turabian Style

Changxun Fang; Pengli Zhang; Lanlan Li; Luke Yang; Dan Mu; Xue Yan; Zhong Li; Wenxiong Lin. 2020. "Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance." , no. : 1.

Preprint content
Published: 26 March 2020
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Background: Rice is a chilling-sensitive crop that would suffer serious damage from low temperatures. Overexpression of the Lsi1 gene (Lsi1-OX) in rice enhances its chilling tolerance. This study revealed that a serine hydroxymethyltransferase (OsSHMT) mainly localised in the endoplasmic reticulum (ER) is involved in increasing tolerance to chilling. Results: A higher transcription level of OsSHMT was detected in Lsi1-OX rice than in the wild type. Histone H1 and nucleic acid binding protein were found to bind to the promoter region of OsSHMT and regulate its expression, and the transcription levels of these proteins were also up-regulated in the Lsi1-OX rice. Moreover, OsSHMT interacts with ATP synthase subunit α, heat shock protein Hsp70, mitochondrial substrate carrier family protein, ascorbate peroxidase 1 and ATP synthase subunit β. Lsi1-encoded protein OsNIP2;1 also interacts with ATP synthase subunit β, and the coordination of these proteins appears to function in reducing reactive oxygen species, as the H2O2 content of transgenic OsSHMT Arabidopsis thaliana was lower than that of the non-transgenic line under chilling treatment. Conclusions: Our results indicate that ER-localised OsSHMT plays a role in scavenging H2O2 to enhance the chilling tolerance of Lsi1-OX rice and that ATP synthase subunit β is an intermediate junction between OsNIP2;1 and OsSHMT.

ACS Style

Changxun Fang; Pengli Zhang; Lanlan Li; Luke Yang; Dan Mu; Xue Yan; Zhong Li; Wenxiong Lin. Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. 2020, 1 .

AMA Style

Changxun Fang, Pengli Zhang, Lanlan Li, Luke Yang, Dan Mu, Xue Yan, Zhong Li, Wenxiong Lin. Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance. . 2020; ():1.

Chicago/Turabian Style

Changxun Fang; Pengli Zhang; Lanlan Li; Luke Yang; Dan Mu; Xue Yan; Zhong Li; Wenxiong Lin. 2020. "Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance." , no. : 1.

Journal article
Published: 04 February 2020 in International Journal of Molecular Sciences
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Inositol polyphosphate 5-phosphatases (5PTases) function in inositol signaling by regulating the catabolism of phosphoinositol derivatives. Previous reports showed that 5PTases play a critical role in plant development and stress responses. In this study, we identified a novel 5PTase gene, Gs5PTase8, from the salt-tolerance locus of chromosome 3 in wild soybean (Glycine soja). Gs5PTase8 is highly up-regulated under salt treatment. It is localized in the nucleus and plasma membrane with a strong signal in the apoplast. Ectopic expression of Gs5PTase8 significantly increased salt tolerance in transgenic BY-2 cells, soybean hairy roots and Arabidopsis, suggesting Gs5PTase8 could increase salt tolerance in plants. The overexpression of Gs5PTase8 significantly enhanced the activities of catalase and ascorbate peroxidase under salt stress. The seeds of Gs5PTase8-transgenic Arabidopsis germinated earlier than the wild type under abscisic acid treatment, indicating Gs5PTase8 would alter ABA sensitivity. Besides, transcriptional analyses showed that the stress-responsive genes, AtRD22, AtRD29A and AtRD29B, were induced with a higher level in the Gs5PTase8-transgenic Arabidopsis plants than in the wild type under salt stress. These results reveal that Gs5PTase8 play a positive role in salt tolerance and might be a candidate gene for improving soybean adaptation to salt stress.

ACS Style

Qi Jia; Song Sun; Defeng Kong; Junliang Song; Lumei Wu; Zhen Yan; Lin Zuo; Yingjie Yang; Kangjing Liang; Wenxiong Lin; Jinwen Huang. Ectopic Expression of Gs5PTase8, a Soybean Inositol Polyphosphate 5-Phosphatase, Enhances Salt Tolerance in Plants. International Journal of Molecular Sciences 2020, 21, 1023 .

AMA Style

Qi Jia, Song Sun, Defeng Kong, Junliang Song, Lumei Wu, Zhen Yan, Lin Zuo, Yingjie Yang, Kangjing Liang, Wenxiong Lin, Jinwen Huang. Ectopic Expression of Gs5PTase8, a Soybean Inositol Polyphosphate 5-Phosphatase, Enhances Salt Tolerance in Plants. International Journal of Molecular Sciences. 2020; 21 (3):1023.

Chicago/Turabian Style

Qi Jia; Song Sun; Defeng Kong; Junliang Song; Lumei Wu; Zhen Yan; Lin Zuo; Yingjie Yang; Kangjing Liang; Wenxiong Lin; Jinwen Huang. 2020. "Ectopic Expression of Gs5PTase8, a Soybean Inositol Polyphosphate 5-Phosphatase, Enhances Salt Tolerance in Plants." International Journal of Molecular Sciences 21, no. 3: 1023.

Original paper
Published: 24 January 2020 in Plant Growth Regulation
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With global climate change in progress, a particular problem is that sudden drops in temperature affect the production of rice. In this study, we explored how to overcome the effects of chilling injury on rice seedlings. Wild-type Dular (WT) plants and Lsi1 (low silicon rice 1) overexpressing Dular plants (Lsi1-OX) were used as research materials. We found that Lsi1 enhanced the antioxidant system and non-structural carbohydrates (NSC) of rice at physiological levels. On this basis, Isobaric Tag for Relative Absolute Quantitation (iTRAQ) technology was used to examine the expression of the rice root proteins in response to low temperature stress. In addition, a total of 433 proteins were differentially expressed in this study, of which 284 were upregulated and 149 were downregulated. These proteins are divided into 24 functions. Moreover, our analysis found that the signaling pathway of the Lsi1-OX plant roots is significantly enhanced under low temperature stress, which in turn, led to the development of resistance reaction and transport pathways, indicating that Lsi1 increases the ability of rice to perceive and transmit low-temperature signals and stimulate the corresponding biochemical processes. In this study, we provided a theoretical basis to understand the mechanism related to the cold resistance of plants and therefor, accelerate the molecular breeding process of low temperature resistant crops.

ACS Style

Zhong Li; Shizhong Feng; Wenshan Zhan; Lining Xu; Changxun Fang; Zhixing Zhang; Wenxiong Lin. Lsi1 plays an active role in enhancing the chilling tolerance of rice roots. Plant Growth Regulation 2020, 90, 529 -543.

AMA Style

Zhong Li, Shizhong Feng, Wenshan Zhan, Lining Xu, Changxun Fang, Zhixing Zhang, Wenxiong Lin. Lsi1 plays an active role in enhancing the chilling tolerance of rice roots. Plant Growth Regulation. 2020; 90 (3):529-543.

Chicago/Turabian Style

Zhong Li; Shizhong Feng; Wenshan Zhan; Lining Xu; Changxun Fang; Zhixing Zhang; Wenxiong Lin. 2020. "Lsi1 plays an active role in enhancing the chilling tolerance of rice roots." Plant Growth Regulation 90, no. 3: 529-543.

Journal article
Published: 06 January 2020 in Chemosphere
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Biochar amendment has been extensively used to improve plant performance and suppress disease in monoculture systems; however, few studies have focused on the underlying control mechanisms of replanting disease. In this study, we assessed the effects of biochar application on Radix pseudostellariae plant growth, rhizosphere soil microbial communities, and the physiological properties of microorganisms in a consecutive monoculture system. We found that biochar addition had little impact on the physiological parameters of tissue cultures of R. pseudostellaria but did significantly mediate microbial abundance in the rhizosphere soil of different consecutive monoculture years, leading to decreases in the abundance of pathogenic Fusarium oxysporum, Talaromyces helicus, and Kosakonia sacchari. Furthermore, biochar amendment had negative effects on the growth of beneficial bacteria, such as Burkholderia ambifaria, Pseudomonas chlororaphis, and Bacillus pumilus. Metabolomic analysis indicated that biochar significantly influenced the metabolic processes of F. oxysporum while inhibiting the mycelial growth and abating the virulence on plants. In summary, this study details the potential mechanisms responsible for the biochar-stimulated changes in the abundances and metabolism of rhizosphere bacteria and fungi, decreases in the contents of pathogens, and therefore improvements in the environmental conditions for plants growth. Further research is needed to evaluate the effects of biochar in long-term field trials.

ACS Style

Hongmiao Wu; Xianjin Qin; Huiming Wu; Feng Li; Jiachun Wu; Ling Zheng; Juanying Wang; Jun Chen; Yanlin Zhao; Sheng Lin; Wenxiong Lin. Biochar mediates microbial communities and their metabolic characteristics under continuous monoculture. Chemosphere 2020, 246, 125835 .

AMA Style

Hongmiao Wu, Xianjin Qin, Huiming Wu, Feng Li, Jiachun Wu, Ling Zheng, Juanying Wang, Jun Chen, Yanlin Zhao, Sheng Lin, Wenxiong Lin. Biochar mediates microbial communities and their metabolic characteristics under continuous monoculture. Chemosphere. 2020; 246 ():125835.

Chicago/Turabian Style

Hongmiao Wu; Xianjin Qin; Huiming Wu; Feng Li; Jiachun Wu; Ling Zheng; Juanying Wang; Jun Chen; Yanlin Zhao; Sheng Lin; Wenxiong Lin. 2020. "Biochar mediates microbial communities and their metabolic characteristics under continuous monoculture." Chemosphere 246, no. : 125835.

Research article
Published: 01 January 2020 in Plant Disease
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Replant disease caused by negative plant-soil feedback commonly occurs in a Pseudostellaria heterophylla monoculture regime. Here, barcoded pyrosequencing of 16S ribosomal DNA amplicons combined with phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis was applied to study the shifts in soil bacterial community structure and functional potentials in the rhizosphere of P. heterophylla under consecutive monoculture and different soil amendments (i.e., bio-organic fertilizer application [MF] and paddy-upland rotation [PR]). The results showed that the yield of tuberous roots decreased under P. heterophylla consecutive monoculture and then increased after MF and PR treatments, which was consistent with the changes in soil bacterial diversity. Both principal coordinate analysis and the unweighted pair-group method with arithmetic means cluster analysis showed the distinct difference in bacterial community structure between the consecutively monocultured soil (relatively unhealthy soil) and other relatively healthy soils (i.e., newly planted soil, MF, and PR). Furthermore, taxonomic analysis showed that consecutive monoculture of P. heterophylla significantly decreased the relative abundances of the families Burkholderiaceae and Acidobacteriaceae (subgroup 1), whereas it increased the population density of families Xanthomonadaceae, Phyllobacteriaceae, Sphingobacteriaceae, and Alcaligenaceae, and Fusarium oxysporum. In contrast, the MF and PR treatments recovered the soil microbiome and decreased F. oxysporum abundance through the different ways; for example, the introduction of beneficial microorganisms (in MF) or the switching between anaerobic and aerobic conditions (in PR). In addition, PICRUSt analysis revealed the higher abundances of membrane transport, cell motility, and DNA repair in the consecutively monocultured soil, which might contribute to the root colonization and survival for certain bacterial pathogens under monoculture. These findings highlight the close association between replant disease of P. heterophylla and the variations in structure and potential functions of rhizosphere bacterial community.

ACS Style

Linkun Wu; Bo Yang; Manlin Li; Jun Chen; Zhigang Xiao; Hongmiao Wu; Qingyu Tong; Xiaomian Luo; Wenxiong Lin. Modification of Rhizosphere Bacterial Community Structure and Functional Potentials to Control Pseudostellaria heterophylla Replant Disease. Plant Disease 2020, 104, 25 -34.

AMA Style

Linkun Wu, Bo Yang, Manlin Li, Jun Chen, Zhigang Xiao, Hongmiao Wu, Qingyu Tong, Xiaomian Luo, Wenxiong Lin. Modification of Rhizosphere Bacterial Community Structure and Functional Potentials to Control Pseudostellaria heterophylla Replant Disease. Plant Disease. 2020; 104 (1):25-34.

Chicago/Turabian Style

Linkun Wu; Bo Yang; Manlin Li; Jun Chen; Zhigang Xiao; Hongmiao Wu; Qingyu Tong; Xiaomian Luo; Wenxiong Lin. 2020. "Modification of Rhizosphere Bacterial Community Structure and Functional Potentials to Control Pseudostellaria heterophylla Replant Disease." Plant Disease 104, no. 1: 25-34.

Journal article
Published: 06 December 2019 in Cells
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Leaf senescence, either as a natural stage of development or as an induced process under stress conditions, incorporates multiple intricate signaling pathways. At the cellular level, retrograde signals have been considered as important players during the initiation and progression of senescence in both animals and plants. The plant-specific single-strand DNA-binding protein WHIRLY1 (WHY1), a repressor of leaf natural senescence, is dually located in both nucleus and plastids. Despite many years of studies, the myth about its dual location and the underlying functional implications remain elusive. Here, we provide further evidence in Arabidopsis showing that alteration in WHY1 allocation between the nucleus and chloroplast causes perturbation in H2O2 homeostasis, resulting in adverse plant senescence phenotypes. The knockout of WHY1 increased H2O2 content at 37 days post-germination, coincident with an early leaf senescence phenotype, which can be rescued by ectopic expression of the nuclear isoform (nWHY1), but not by the plastid isoform (pWHY1). Instead, accumulated pWHY1 greatly provoked H2O2 in cells. On the other hand, exogenous H2O2 treatment induced a substantial plastid accumulation of WHY1 proteins and at the same time reduced the nuclear isoforms. This H2O2-induced loss of nucleus WHY1 isoform was accompanied by enhanced enrichments of histone H3 lysine 9 acetylation (H3K9ac) and recruitment of RNA polymerase II (RNAP II) globally, and specifically at the promoter of the senescence-related transcription factor WRKY53, which in turn activated WRKY53 transcription and led to a senescence phenotype. Thus, the distribution of WHY1 organelle isoforms and the feedback of H2O2 intervene in a circularly integrated regulatory network during plant senescence in Arabidopsis.

ACS Style

Wenfang Lin; Dongmei Huang; Ximiao Shi; Ban Deng; Yujun Ren; Wenxiong Lin; Ying Miao. H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis. Cells 2019, 8, 1585 .

AMA Style

Wenfang Lin, Dongmei Huang, Ximiao Shi, Ban Deng, Yujun Ren, Wenxiong Lin, Ying Miao. H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis. Cells. 2019; 8 (12):1585.

Chicago/Turabian Style

Wenfang Lin; Dongmei Huang; Ximiao Shi; Ban Deng; Yujun Ren; Wenxiong Lin; Ying Miao. 2019. "H2O2 as a Feedback Signal on Dual-Located WHIRLY1 Associates with Leaf Senescence in Arabidopsis." Cells 8, no. 12: 1585.