Adipose‐derived stem cells (ADSCs) have been documented as possible candidates for skin rejuvenation. However, the effects of ADSC‐derived exosomes on photoaged skin remain to be fully elucidated. This study was aimed at determining the antiaging effects of ADSC‐derived exosomes on photoaged skin. Human ADSCs were isolated from the adipose tissue of healthy women and cultured in vitro. Then, exosomes were extracted from the cultured ADSCs, purified by ultracentrifugation, and verified by examination of cell morphology using transmission electron microscopy and the identification of specific biomarkers. Meanwhile, the optimal exosome concentration and treatment time were selected. The photoaged skin model was created by subjecting Sprague‐Dawley rats to ultraviolet B radiation. Exosomes were injected into the photoaged skin in a single therapeutic dose. The thickness of the epidermis and dermis was observed by HE staining. The relative mRNA expression of type I collagen, type III collagen, and matrix metalloproteinases (MMP‐1 and MMP‐3) was determined by real‐time PCR. In the rat model of photoaged skin, the injected exosomes markedly decreased the epidermal thickness and increased the dermal thickness of the photoaged skin 7 days after treatment. Moreover, the proportion of the stratum corneum of the epidermis was decreased. Furthermore, real‐time RT‐PCR showed that the mRNA expression of type I collagen was increased and that of type III collagen, MMP‐1, and MMP‐3 was decreased. Our results demonstrate that ADSC‐derived exosome treatment could significantly improve skin photodamage and that ADSC‐derived exosomes may be a potential agent for photoaged skin treatment.

Cesarean section results in scarring, which usually leads to adhesion between the subcutaneous fat and the abdominal wall muscle. The present study aimed to evaluate the therapeutic effect of autologous fat grafting on scar adhesion to the abdominal wall after cesarean section. Thirty-six patients with scar adhesion to the abdominal wall after cesarean section were recruited and treated between October 2013 and December 2015. The adhesion between the subcutaneous fat and the abdominal wall muscle was carefully separated through a small incision in the original scar to form multiple subcutaneous tunnels. Aspirated fat was injected into the scar lesion and subcutaneous tunnels, and the wound was then sutured. The clinical outcome was evaluated by comparing the pretreatment and 1-year posttreatment photographs and Patient and Observer Scar Assessment Scale (POSAS) scores. All patients had a marked improvement in the appearance, texture, and depression of the scar during 12 months of follow-up. The 1-year posttreatment POSAS scores for the color, pain, pruritus, hardness, fullness, mobility, and appearance of the scar were significantly decreased compared with the pretreatment scores. Hematoxylin-eosin staining revealed adipocyte-like cells in treated scar tissue specimens obtained 1 year after treatment. None of the patients reported severe adverse reactions. Autologous fat grafting combined with adhesion release may be a good treatment option for abdominal wall scarring after cesarean section. This method is minimally invasive and effective in achieving good functional and esthetic outcomes.

ObjectiveThe aim of this study was to assess the CT findings of chest lesions in patients with systemic lupus erythematosus (SLE), and to explore its correlation with the age and serum markers (anti-dsDNA antibodies, anti-Sm antibodies, CRP, ESR, C3, C4 and IgG), in an attempt to speculate the possible mechanism of chest lesions in SLE patients.Materials and methodsThirty nine patients who met the SLE classification criteria were enrolled into this study. All patients underwent serological tests and chest CT examination. The chest CT findings of each patients were analyzed, and its correlation with the age, serum markers were explored. All data were analyzed by using SPSS 19.0 software. Two-sample t-test was used to analyze the age difference between the two groups with or without chest lesions. Relationship between the serum markers and the chest lesions was analyzed by using the Fisher's exact probability method.ResultsAll the 39 patients (34 females and 5 males), aged from 19 to 74 years old, the mean age was 44.13 ± 12.17 years old. Among the serum markers, the positive rate of C3 was the highest (79.49%), ESR positive in 22 patients (56.41%), 9 patients of CRP positive (23.08%). Of all the 39 patients, abnormal manifestations of chest CT were found in 29 patients (74.36%), the most common changes were pulmonary interstitial changes (66.67%), the second were mediastinal/pleural changes (61.54%), then were the pulmonary parenchymal changes (25.64%) and pulmonary vascular changes (12.82%). ESR positive rate difference was found between the two groups with or without pulmonary parenchymal lesions (P < 0.05), and CRP positive rate difference was found between the two groups with or without mediastinal/pleural lesions (P < 0.05).ConclusionChest involvement occurred with high frequency in SLE patients, and the CT manifestations were complex and various, pulmonary interstitial lesions were the most common. Patients with ESR positive were more susceptible to have pulmonary parenchymal lesions, and mediastinal/pleural lesions were more common in patients with CRP positive