Despite the trend of a transition to “clean” energy, the coal industry still plays a significant role in the global economy. The constant need for raw materials and energy for production leads to an environmental crisis—an increase in the content of greenhouse gases in the atmosphere, especially in the mining regions. The purpose of this study was to analyze the impact of the carbon footprint on the environment and to study ways to reduce the negative impact of coal mining enterprises on the ecology. To analyze the chosen topic, the available reviews and research articles on the impact of the carbon footprint of coal mining enterprises, and the ways to reduce it and restore the biodiversity of wastelands, were used. It was found out that a complete ban on the extraction and use of coal in the industry will not lead to the desired result. The main ways to reduce the negative impact of coal mining enterprises on the environment were considered. The most promising direction for reducing the carbon footprint is the restoration of the vegetation cover by phytoremediation methods and the creation of carbon landfills in reclaimed territories in technogenically polluted coal mining regions.

In order to ensure the timely and uninterrupted supply of medicinal plant raw materials, the methods of cultivation of plant cell cultures, namely, the production of plant root cultures, are relevant. In this paper, the geroprotective potential of Hedysarum neglectum Ledeb and Panax ginseng C. A. Mey root cultures is studied. They were cultured under in vitro conditions by transforming the rhizome (H. neglectum) and seed seedlings (P. ginseng) with Agrobacterium rhizogenes 15834 Swiss. To identify the geroprotective potential, the antimicrobial disc-diffusion method and the antioxidant activity were analyzed by titration of KMnO4 extracts of plant root cultures. The qualitative and quantitative composition was analyzed using high-performance liquid chromatography, thin-layer chromatography, and gas chromatography with mass spectrometry. In the course of the work, the presence of antimicrobial and antioxidant activity of plant root culture extracts was established. Biologically active substances contained in extracts of Hedysarum neglectum Ledeb root crops and Panax ginseng C. A. Mey are characterized by geroprotective potential, so they can act as a source of natural antioxidants in the functional nutrition of the geroprotective orientation.

Wheat is a vital agricultural crop whose phytopathogens include fungi of the genera Fusarium and Alternaria. Synthetic pesticides, which are used to combat them, have a negative impact on the environment. Therefore, there is a need for developing safe and effective biopesticides. We aimed to create a consortium of extremophilic microorganisms isolated from natural sources to protect wheat from the diseases caused by Alternaria and Fusarium fungi. Ten isolates of extremophilic microorganisms were tested for their antimicrobial activity against Escherichia coli and their antagonistic activity against phytopathogens. Based on the results, we developed microbial consortia and evaluated their effectiveness in protecting wheat from phytopathogens. Five of the strains under study showed the highest activity, three of which were biocompatible, namely Leclercia sp., Sphingomonas paucimobilis, and Lactobacillus plantarum. Four consortia were created from these microorganisms, of which consortium B (with a 2:1:1 ratio of the strains, respectively) proved the most effective. In particular, it increased the area free from the phytopathogen by 4.2% compared to the average values of its individual microorganisms. Also, the consortium had a phytostimulating effect on wheat seedlings (germination of 73.2–99.6%) and protected the seeds infected with phytopathogens from morphometric changes. The resulting consortium can be used as a biopesticide since it is highly effective in protecting wheat from Alternaria and Fusarium pathogens.

Introduction. Coal mining is the main source of anthropogenic impact on the landscapes of the Kemerovo Region – Kuzbass. The current mine reclamation rate lags far behind the annual increase in disturbed lands. A reclamation fund can be a perfect solution to this relevant issue. The present research objective was to analyze and structure the available data on the anthropogenic impact of coal mining in Kuzbass. The article reviews new effic ient methods of reclamation and resoiling. Study objects and methods. The study featured ten years of research publications that were registered in the PubMed database of the National Center for Biotechnology Information (USA), Elsevier (Scopus, ScienceDirect), the Web of Science, and the Russian Electronic Library (eLibrary.ru). Results and discussion. The research revealed the following Kuzbass districts that experience the greatest mining impact: Novokuznetsk, Prokopyevsk, Kemerovo, Belovo, and Leninsk-Kuznetskiy. The authors also identified the most common pollutants associated with coal mining. Polycyclic aromatic hydrocarbons (PAHs) appeared to be the most dangerous pollutants: as waste coal burns, these substances cover considerable distances with the wind. Biodegradation seems to be the optimal solution because PAHs are known to be carcinogenic, and most mine tips are located near settlements. The article also features mine reclamation laws and introduces a list of plants with a high absorption capacity recommended for biological reclamation, as well as microorganisms and their consortia used for bioremediation. Conclusion. The authors identified the most promising methods of mine reclamation in the Kemerovo region, i.e. bioremediation with pollutant-binding microbial consortia and plants.

Wheat (Triticum L.) is a strategically important agricultural crop because its quality and yield provide food security for the population. Biological fertilizers improve the growth and development of agricultural crops. Unlike chemical ones, they have no toxic effect on people and the environment. This research assessed the positive effect of extremophilic microorganisms isolated from coal dump soils of the Kemerovo Region (Russia) on the growth and development of wheat. The study featured bacterial isolates of Achromobacter denitrificans, Klebsiella oxytoca, and Rhizobium radiobacter, as well as their consortia in four different ratios: 1:1:1 (Consortium A), 2:1:1 (Consortium B), 1:2:1 (Consortium C), 1:1:2 (Consortium D), respectively. The beneficial effect was assessed by determining such factors as nitrogen fixation, solubilization of phosphates, potassium, and zinc, and production of gibberellic acid, siderophores, and hydrogen cyanide. The wheat samples were checked for germination, root length, and stem length. R. radiobacter demonstrated the best nitrogen fixation properties. Consortium D, with two shares of R. radiobacter, yielded the best results for zinc solubilization. R. radiobacter proved to be the most efficient potassium solubilizer while the isolate of A. denitrificans was the best phosphate solubilizer. The largest amount of gibberellic acid belonged to K. oxytoca. Consortium C, which included two shares of this isolate, appeared to be the most effective siderophore producer. All samples but A. denitrificans were able to produce hydrogen cyanide. The best seed germination rate (84%) belonged to Consortium C, which contained a double share of K. oxytoca. Consortia C and B (two shares of A. denitrificans) had the greatest positive effect on the root length. Treatment with Consortium B resulted in the longest average stem length. Extremophilic microorganisms isolated from coal dump soils of the Kemerovo Region (Russia) had a good potential as biofertilizers that could improve wheat quality and local food security.