Telah dilakukan penelitian tentang aktivitas antibakteri ekstrak etanol sabut kelapa (Cocos nucifera Linn) terhadap bakteri Staphylococcus aureus dan Escherichia coli, bertujuan untuk menentukan konsentrasi etanol terbaik yang dapat menghasilkan ekstrak sabut kelapa muda, setengah tua, dan sabut kelapa tua dengan aktivitas antibakteri yang tinggi. Konsentrasi etanol yang diterapkan untuk mengekstrak sabut kelapa antara lain 75%, 80%, 85%, 90% dan 95% sedangkan Pengujian aktivitas antibakteri dilakukan dengan menerapkan metode sumur difusi dan parameter yang diamati adalah diameter zona hambat. Hasil penelitian menunjukan bahwa daya hambat terbaik ekstrak sabut kelapa terhadap bakteri Staphylococcus aureus dan Escherichia coli adalah 95% dengan diameter zona hambat masing-masing 22,59 mm dan 27,13 mm. Kontrol positif yang digunakan adalah kloramfenikol dan menghambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli dengan diameter zona hambat masing-masing 28,66 mm dan 34,60 mm, sedangkan kontrol negatif menggunakan etanol dan zona hambat keduanya nol.Kata kunci : Ekstrak Etanol Sabut kelapa, Staphylococcus aureus, Escherichia coli, Diameter Zona Hambat, Aktivitas Antibakteri.

This study reported the isolated compound from vetiver grass-root (Vetiveria zizanioides Stapf) have bioactivity as a repellant against termites. The compounds were extracted by n-hexane solvent using the soxhletation method. The compounds were tested through bioactivity assay using wood pieces added isolated compound as a sample on the final assay, acetone as a blank, and wood pieces without treatment as a control. The result showed that the isolated compound was acquired by separation and purification in the form of colorless oil 0.0225 g. Of the thin-layer chromatography (TLC) assay using eluents of n-Hexane eluent 100%, n-Hexane : DCM 95%, and n-Hexane : Acetone 70%, were obtained a red-purple spot and Rf values which are 0.27, 0.59, and 0.91, respectively. 2-methyl butane-1-amine (C₅H₁₃N) was supposed by the structure elucidation of the isolated compound. The molecular weight was 87.1 g/mol as a precursor terpenoid based on the spectrum examination of FTIR and GC-MS. The bioactivity showed that an isolated compound has acted as a repellent against termite (Cryptotermes sp.) with % attractive (0.025 %) and attractiveness index (AI) (-0.039). A negative value of AI indicated that the compound is potential as a repellent against termites.

For the majority of farmers, the limited availability of subsidized fertilizers in Rukti Endah Village, Seputih Raman District, poses a problem because it lowers agricultural production. Academics at the University of Lampung initiated a socialization about organic fertilizer production to solve this problem. The aim of this community service is to train and educate people on how to make organic fertilizer from kitchen waste in order to overcome restrictions on fertilizer subsidies. The methods used in community service activities are lectures, demonstrations of making compost and group discussions attended by 30 participants. The activity's outcomes revealed an increase in community knowledge of the process of transforming waste into compost and liquid organic fertilizer in Rukti Endah Village (60.6%). The final products have been used to support agricultural production in Rukti Endah Village and the area around it.

Insecticides are a type of pesticide that is commonly used by farmers to eradicate insect pests, one of which is the stink bug. Excessive use results in resistance and contamination of soil, water, and air. Another alternative is bioinsecticides which are safe and do not cause resistance. In this study, the samples used were gadung and bintaro root extracts. The method used is maceration, after the maceration process, the filtrate will be tested on the target animal, looking for the best insecticide mortality and screening for phytochemicals. Lastly, the best bioinsecticide solutions were identified using FT-IR and UV-Vis spectrophotometers. The best bioinsecticide solutions with 60% and 40% mortality were bioinsecticides with a concentration of 70% gadung and 30% gadung 20% bintaro. Phytochemical screening tests were carried out on both of them, the results were positive for terpenoids and alkaloids. Identification using the FTIR spectrophotometer showed that the bioinsecticide solution contained secondary metabolites with O-H groups appearing at absorption wave number 3260 cm-1, C=O groups appearing at wave number 1637cm-1,, and C-H groups appearing at wave number 1359 cm-1. The results of the UV-Vis spectrum showed that the bioinsecticide solution had the C=C functional group as indicated by the appearance of peaks at a wavelength of 210 nm, 217 nm, and 223 nm. Insektisida ialah salah satu jenis pestisida yang umum digunakan oleh para petani untuk membasmi hama serangga, salah satunya yakni walang sangit. Penggunaan secara berlebih mengakibatkan resisten dan pencemaran tanah, air dan udara. Alternatif lain yakni bioinsektisida yang aman dan tidak menimbulkan resisten. Pada penelitian ini sampel yang digunakan yakni ekstrak umbi gadung dan bintaro. Metode yang digunakan yakni maserasi, setelah proses maserasi, filtrat akan diuji ke hewan target, dicari mortalitas walang sangit terbaik dan diskrining fitokimia. Terakhir larutan bioinsektisida terbaik diidentifikasi dengan spektrofotometer FT-IR dan UV-Vis. Hasil larutan bioinsektisida terbaik dengan mortalitas 60% dan 40% yakni bioinsektisida dengan konsentrasi 70% gadung dan 30% gadung 20% bintaro. Uji skrining fitokimia dilakukan pada keduanya hasilnya positif mengandung terpenoid dan alkaloid. Identifikasi menggunakan spektrofotometer FTIR menunjukkan bahwa larutan bioinsektisida mengandung metabolit sekunder dengan gugus O-H yang muncul pada serapan bilangan gelombang 3260 cm-1, gugus C=O yang muncul pada bilangan gelombang 1637cm-1 dan gugus C-H muncul pada bilangan gelombang 1359 cm-1. Hasil Spektrum UV-Vis menunjukkan larutan bioinsektisida memiliki gugus fungsi C=C yang ditunjukkan dengan munculnya puncak pada panjang gelombang 210 nm, 217 nm dan 223 nm.

Cyperus rotundus Linn is a plant that is historically used in traditional medicine with anti-cancer potential. Despite the evidence of C. rotundus anti-cancer effect on various human carcinoma cell lines, its pharmacological mechanism remains unclear, particularly its non-polar fraction. This study was employed to provide mechanistic insight regarding the anti-cancer properties of C. rotundus non-polar fraction by integrating in silico and in vitro approach. The network pharmacology study was used to observe the molecular targets of n-hexane fraction of C. rotundus, confirmed by molecular docking simulation using Autodock 4.2. The in vitro toxicity using BSLT method was used to strengthen the in silico result. The network pharmacology investigation revealed several core targets including PI3K, MAPK1, mTOR, RAF1, and NF-κB in the potential anti-cancer mechanism of C. rotundus. The molecular docking study illustrated that compound 3 (Isopetasol) and compound 9 (alpha-cyperone) as the most promising compound in n-hexane fraction of C. rotundus, with free binding energies consistently less than -7 kcal/mol in all targets. The in vitro BSLT signified the in silico results, highlighting the highest toxicity of fraction 3 exhibited among others. Integrating the network pharmacology and molecular docking simulation along with in vitro toxicity have provided evidence of the anti-cancer potential of n-hexane fraction of C. rotundus. Specific compounds and the molecular targets responsible for its anti-cancer properties have been identified, warranting further investigations.

One of the main problems faced by farmers in Lampung today is the limited or scarcity of subsidized fertilizers. Meanwhile, there is an interesting phenomenon that has occurred on campus, including at FMIPA, University of Lampung (Unila), that about waste. So far, organic waste in the Faculty of Mathematics and Natural Sciences, Unila, has not been utilized optimally. The organic waste is only collected and deposited at the Integrated Waste Management Site (TPST) at Unila to be sorted and then taken to the Final Disposal Site (TPA) of Bandar Lampung City. These conditions prompted us, as academics at the University of Lampung, to be moved to carry out a community service program with the theme "Conversion of organic waste in the Faculty of Mathematics and Natural Sciences at the University of Lampung into Liquid Organic Fertilizer (POC) using solid liquid (SL) techniques." This PKM activity aims to process organic waste into liquid organic fertilizer. The processing of organic waste into liquid organic fertilizer is carried out using the soluble liquid technique, in which the organic waste is chopped and then pressed using a press machine, the pressed liquid is added to the starter solution so that the solid particles can dissolve. The dregs resulting from the press are also added to the starter solution so that the dregs can be used as animal feed or compost. The resulting product is applied to plants around FMIPA Unila. The results of this PKM activity were in the form of 60 liters of liquid organic fertilizer (POC) from 100 kg of organic waste, in other words, a yield of 60% was obtained. The resulting POC was then applied to plants in the FMIPA environment. Apart from products in the form of POC, this activity was also able to slowly change the behavior of the Unila FMIPA academic community towards existing organic waste. It is hoped that regular coaching will change the FMIPA environment to be cleaner, healthier and greener. The liquid organic fertilizer produced can also be packaged to be further sold to farmers at affordable prices, so as to increase the economic value of organic waste which has not been utilized so far and also increase the income of some of the education staff in FMIPA Unila.