This paper presents a Peano fractal geometry complementary split ring resonator (PFCSRR) loaded microstrip transmission line with a microfluidic channel for equol (EQ) sensing in a high and wide range of concentrations in aqueous solution. The proposed sensor was designed based on a CSRR loaded microstrip line with a Peano fractal in the center of a CSRR and validated through simulation and experiment. The microfluidic channel was fabricated using polydimethylsiloxane (PDMS) and installed to cover the sensing area. The free space, empty microfluidic channels, deionized (DI) water, dimethyl sulfoxide (DMSO), and various concentrations of EQ were measured by a microwave sensor through sample-filled microfluidic channels. Detection of high levels of EQ was in the concentration range of 0.01 mM – 100 mM. The materials under test (MUTs) were measured in the frequency range of 1.0 GHz–3.5 GHz based on the magnitude of the transmission coefficient (S ₂₁ ) and resonance frequency (F _r ) at room temperature. The S ₂₁ and F _r were recorded and analyzed by logarithmic concentrations of EQ for the determinant of the correlations between EQ concentration and S ₂₁ and F _r . Principal component analysis (PCA) and K–means clustering were used to analyze and classify groups of MUTs.

The global issue of pollution caused by endocrine-disrupting chemicals (EDCs) has been gaining increasing attention. Among the EDCs of environmental concern, 17β-estradiol (E2) can produce the strongest estrogenic effects when it enters the organism exogenously through various routes and has the potential to cause harm, including malfunctions of the endocrine system and development of growth and reproductive disorders in humans and animals. Additionally, in humans, supraphysiological levels of E2 have been associated with a range of E2-dependent disorders and cancers. To ensure environmental safety and prevent potential risks of E2 to human and animal health, it is crucial to develop rapid, sensitive, low cost and simple approaches for detecting E2 contamination in the environment. A planar microwave sensor for E2 sensing is presented based on the integration of a microstrip transmission line (TL) loaded with a Peano fractal geometry with a narrow slot complementary split-ring resonator (PF-NSCSRR) and a microfluidic channel. The proposed technique offers a wide linear range for detecting E2, ranging from 0.001 to 10 mM, and can achieve high sensitivity with small sample volumes and simple operation methods. The proposed microwave sensor was validated through simulations and empirical measurements within a frequency range of 0.5–3.5 GHz. The E2 solution was delivered to the sensitive area of the sensor device via a microfluidic polydimethylsiloxane (PDMS) channel with an area of 2.7 mm² and sample value of 1.37 µL and measured by a proposed sensor. The injection of E2 into the channel resulted in changes in the transmission coefficient (S₂₁) and resonance frequency (F_r), which can be used as an indicator of E₂ levels in solution. The maximum quality factor of 114.89 and the maximum sensitivity based on S₂₁ and F_r at a concentration of 0.01 mM were 1746.98 dB/mM and 40 GHz/mM, respectively. Upon comparing the proposed sensor with the original Peano fractal geometry with complementary split-ring (PF-CSRR) sensors without a narrow slot, several parameters were evaluated, including sensitivity, quality factor, operating frequency, active area, and sample volume. The results showed that the proposed sensor exhibited an increased sensitivity of 6.08% and had a 40.72% higher quality factor, while the operating frequency, active area, and sample volume showed decreases of 1.71%, 25%, and 28.27%, respectively. The materials under tests (MUTs) were analyzed and categorized into groups using principal component analysis (PCA) with a K-mean clustering algorithm. The proposed E2 sensor has a compact size and simple structure that can be easily fabricated with low-cost materials. With the small sample volume requirement, fast measurement with a wide dynamic range, and a simple protocol, this proposed sensor can also be applied to measure high E2 levels in environmental, human, and animal samples.