The Tibetan Plateau (TP) is the largest alpine plateau on Earth and plays an important role in global climate dynamics. On the TP, climate change is happening particularly fast, with an increase in air temperature twice the global average. The particular sensitivity of this high mountain environment allows observation and tracking of abiotic and biotic feedback mechanisms. Closed lake systems, such as Nam Co on the central TP, represent important natural laboratories for tracking past and recent climatic changes, as well as geobiological processes and interactions within their respective catchments. This review gives an interdisciplinary overview of past and modern environmental changes using Nam Co as a case study. In the catchment area, ongoing rise in air temperature forces glaciers to melt, contributing to a rise in lake level and changes in water chemistry. Some studies base their conclusions on inconsistent glacier inventories, but an ever-increasing deglaciation and thus higher water availability have persisted over the last few decades. Increasing water availability causes translocation of sediments, nutrients and dissolved organic matter to the lake, as well as higher carbon emissions to the atmosphere. The intensity of grazing has an additional and significant effect on CO₂ fluxes, with moderate grazing enhancing belowground allocation of carbon while adversely affecting the C sink potential through reduction of above-surface and subsurface biomass at higher grazing intensities. Furthermore, increasing pressure from human activities and livestock grazing are enhancing grassland degradation processes, thus shaping biodiversity patterns in the lake and catchment. The environmental signal provided by taxon-specific analysis (e.g., diatoms and ostracods) in Nam Co revealed profound climatic fluctuations between warmer–cooler and wetter–drier periods since the late Pleistocene and an increasing input of freshwater and nutrients from the catchment in recent years. Based on the reviewed literature, we outline perspectives to further understand the effects of global warming on geodiversity and biodiversity and their interplay at Nam Co, which acts as a case study for potentially TP-level or even worldwide processes that are currently shaping high mountain areas.

The last 85,000 years were characterized by high climate and environmental variability on the Yucatán Peninsula. Heinrich stadials are examples of abrupt climate transitions that involved shifts in regional temperatures and moisture availability. Thus, they serve as natural experiments to evaluate the contrasting responses of aquatic and terrestrial ecosystems. We used ostracodes and pollen preserved in a 75.9-m-long sediment core (PI-6, ~85 ka) recovered from Lake Petén Itzá, Guatemala, to assess the magnitude and velocity of community responses. Ostracodes are sensitive to changes in water temperature and conductivity. Vegetation responds to shifts in temperature and the ratio of evaporation to precipitation. Ostracodes display larger and more rapid community changes than does vegetation. Heinrich Stadial 5-1 (HS5-1) was cold and dry and is associated with lower ostracode and vegetation species richness and diversity. In contrast, the slightly warmer and dry conditions during HS6 and HS5a are reflected in higher ostracode species richness and diversity. Our paleoecological study revealed the greatest ecological turnover for ostracodes occurred from 62.5 to 51.0 ka; for pollen, it was at the Pleistocene/Holocene transition. Future studies should use various climate and environmental indicators from lake and marine sediment records to further explore late glacial paleoclimate causes and effects in the northern neotropics.

We evaluated how ranges of four endemic and non-endemic aquatic ostracode species changed in response to long-term (glacial–interglacial cycles) and abrupt climate fluctuations during the last 155 kyr in the northern Neotropical region. We employed two complementary approaches, fossil records and species distribution models (SDMs). Fossil assemblages were obtained from sediment cores PI-1, PI-2, PI-6 and Petén-Itzá 22-VIII-99 from the Petén Itzá Scientific Drilling Project, Lake Petén Itzá, Guatemala. To obtain a spatially resolved pattern of (past) species distribution, a downscaling cascade is employed. SDMs were reconstructed for the last interglacial (∼120 ka), the last glacial maximum (∼22 ka) and the middle Holocene (∼6 ka). During glacial and interglacial cycles and marine isotope stages (MISs), modelled paleo-distributions and paleo-records show the nearly continuous presence of endemic and non-endemic species in the region, suggesting negligible effects of long-term climate variations on aquatic niche stability. During periods of abrupt ecological disruption such as Heinrich Stadial 1 (HS1), endemic species were resilient, remaining within their current areas of distribution. Non-endemic species, however, proved to be more sensitive. Modelled paleo-distributions suggest that the geographic range of non-endemic species changed, moving southward into Central America. Due to the uncertainties involved in the downscaling from the global numerical to the highly resolved regional geospatial statistical modelling, results can be seen as a benchmark for future studies using similar approaches. Given relatively moderate temperature decreases in Lake Petén Itzá waters (∼5 ^∘C) and the persistence of some aquatic ecosystems even during periods of severe drying in HS1, our data suggest (1) the existence of micro-refugia and/or (2) continuous interaction between central metapopulations and surrounding populations, enabling aquatic taxa to survive climate fluctuations in the northern Neotropical region.

Dwelling in a variety of aquatic habitats, one of the most abundant groups of microcrustaceans, ostracodes, are widely used indicator organisms in paleolimnological research. Typically, they are identified via traditional methods using morphological features but this may be excessively time‐consuming and prone to inter‐investigator variation. DNA barcoding and metabarcoding have become important tools for specimen identification, with a great impact in the field of taxonomy, (paleo‐)ecology and evolution. Despite its potential, metabarcoding has been rarely used to analyze the community structure of ostracodes. Here, we evaluate the performance of a metabarcoding approach for ostracode identification in surface sediment samples collected from Lake Nam Co on the Tibetan Plateau. We tested six different primer pairs amplifying fragments of three different genes, and compared their success in inferring ostracode communities, coupled with morphological identification of ostracodes from the same sediment samples. In total, depending on the primer pair used, seven to nineteen ostracode amplicon sequence variants (ASVs) were identified. Via microscopy, eight morphospecies were identified. We found considerable differences between primer pairs in yielding ostracode sequences via metabarcoding. In general, the highest proportions of ostracode reads and ASVs were found with primers amplifying fragments of the 18S rRNA gene, whereas primers for COI gene had the highest in silico amplification success and highest sequencing depth per sample but only contained <1% of ostracode sequences. As a consequence, the metabarcoding results with 18S rRNA gene were more consistent with the morphological data compared to those obtained with COI or mitochondrial 16S rRNA primers. No significant effects of treatment with different sediment quantities for DNA extraction (10 g vs. 0.5 g) were found on ostracode ASVs community composition. These results indicate that DNA metabarcoding can serve as an efficient tool for ostracode‐based environmental reconstructions but requires an informed decision on primers and target gene, as well as extending the barcoding database for improved accuracy.

The recent rise in air temperatures detected at high altitudes of the Tibetan Plateau has accelerated glacier melt and retreat. Moreover, enhanced monsoonal precipitation has increased runoff and transport of allochthonous material to the lakes. Consequently, water levels are rising, modifying the spatial distribution and composition of local aquatic biota. To infer these environmental and biological changes in recent decades, a 30‐cm‐long sediment core, representing the past ~160 years, from Nam Co, an endorheic lake, was analyzed for subfossil chironomid assemblages and sediment geochemistry. In total, 25 chironomid morphotypes were identified. Nineteen were considered as non‐rare taxa (abundances ≥2%) and six as rare taxa (abundances <2%). Since 1956 ce, higher chironomid richness (S = 19) is evident compared to the previous 100 years. The simultaneous decrease in the abundance of profundal Micropsectra radialis‐type and increase of both Chironomus and Procladius, taxa adapted to more eurytopic and slightly warmer water bodies, indicate increasing water temperatures and intensified primary productivity. The dominance of littoral chironomid assemblages reflects increasing lake water levels, flooded shorelines and expansion of littoral areas driven by increased precipitation and glacial meltwater input both resulting from the increase in air temperatures. This scenario is confirmed by increases in total nitrogen and Zr/Rb ratios, indicating higher productivity and coarser grain size as a consequence of increased runoff via the Niya Qu. These hydrological changes have resulted in a positive water balance that can be linked to an increase in moisture supply from the Indian summer monsoon and glacier melt, reflecting increasing temperatures and precipitation since 1956 ce, ultimately driven by anthropogenic warming.

Taxonomic clarification of Tonnacypris stewarti comb. nov. (= Herpetocypris stewarti), a non-marine ostracod first described by Daday in 1908, was required due to the existence of various synonyms without detailed morphological descriptions. Our study examined specimens from Mang-tsa, Nam Co (Tibetan Plateau) and Lake Band-e Amir (Afghanistan). We identified seven definitive synonyms (Herpetocypris stewarti Daday 1908, Ilyodromus estonicus Järvekülg 1960, Eucypris afghanistanensis Hartmann 1964, E. gyirongensis Yang 1982, E. rischtanica Schneider 1963 in Mandelstam & Schneider 1963, Tonnacypris estonica (Järvekülg 1960), and T. gyirongensis (Guo et al. 2016). After a detailed study of the morphology of valves and soft parts supplemented by a literature review, we propose combining the genus Tonnacypris with the specific epithet stewarti, resulting in the new name Tonnacypris stewarti. We documented the ontogenetic series of T. stewarti in surface sediment samples from Nam Co. Only female specimens were found, consistent with observations in Mongolia, where they were considered asexual populations. Sexual populations were reported from Afghanistan and the southern Tibetan Plateau, suggesting geographic parthenogenesis in the Palearctic region. Because of widely missing males, we explored the use of female genital lobes as a distinguishing feature for taxonomy. Considering current ecological knowledge, T. stewarti could serve as a (paleo)—bioindicator of cold, oligotrophic, and fresh to slightly brackish habitats, including rivers, lagoons, and lakes. This study emphasizes the importance of resolving taxonomic issues for future paleolimnological research. Moreover, our investigation highlights the potential use of sclerotized internal female genital lobes as distinctive characteristics for species differentiation, especially in asexual populations.