Species richness of terrestrial isopods is high in caves of the Dinaric Karst, which hosts ~10% of the world’s nominal oniscidean troglobionts. The most widespread taxon is the southern European genus Alpioniscus, which consists of two subgenera: Alpioniscus s.s. and Illyrionethes. Before this study, 14 nominal troglobiotic Illyrionethes taxa were recorded from the Dinaric Karst. Our molecular analyses using two mitochnodrial DNA (16S rRNA and COI) fragments and a nuclear gene (H3) fragment on all known Dinaric taxa identified three distinct lineages: strasseri-, heroldi- and magnus-lineage. Our results confirmed the validity of most nominal species. The exceptions are Alpioniscus balthasari, which consists of two different species including Alpioniscus iapodicus, and Alpioniscus heroldi, which is paraphyletic with respect to Alpioniscus bosniensis. The strasseri-lineage was highly supported by all phylogenetic methods used; therefore, we performed a detailed morphological analysis to distinguish and characterize the species of this group. New morphological characters, such as body part ratios, are proposed for future species identification. In addition, we redescribe three known species (Alpioniscus strasseri, Alpioniscus christiani and Alpioniscus balthasari) and describe two new ones (Alpioniscus hirci sp. nov. and Alpioniscus velebiticus sp. nov.). As a result, 15 nominal species of Illyrionethes are currently known from the Dinaric Karst.

The Dinaric Karst is a global hotspot for subterranean diversity, with two distinct peaks of species richness in the northwest and southeast, and an area of a lower species richness in the central part. In this article, we present a species list and describe the ecological conditions of the Lukina jama–Trojama cave system, located in the central part of the Dinaric Karst. This cave system is the deepest and one of the most logistically challenging cave systems sampled so far in the Dinaric Karst. Repeated sampling resulted in a list of 45 species, including 25 troglobionts, 3 troglophiles, 16 stygobionts, and 1 stygophile. Most of the recorded species are endemic to the Velebit Mountain, while three species are endemic to the Lukina jama–Trojama cave system. Within the system, species richness peaks in the deepest third of the cave, most likely reflecting the harsh ecological conditions in the upper parts, including ice, cold winds, and occasional waterfalls. Milder and more stable deeper parts of the cave contain a rich subterranean species community, part of which is associated with two very distinct aquatic habitats, the cave hygropetric and the phreatic zone. The newly recognized hotspot of subterranean biodiversity in the central Dinaric Karst, which has emerged between the two known centers of biodiversity, further highlights the species richness in large cave systems, but also challenges the diversity patterns in the Dinaric Karst overall.

Terrestrial isopods were studied in the Dubravica peat bog and surrounding forest in the northwestern Croatia. Sampling was conducted using pitfall traps over a

While extensive research on traditional model species has significantly advanced the biological sciences, the ongoing search for new model organisms is essential to tackle contemporary challenges such as human diseases or climate change, and fundamental phenomena including adaptation or speciation. Recent methodological advances such as next-generation sequencing, gene editing, and imaging are widely applicable and have simplified the selection of species with specific traits from the wild. However, a critical milestone in this endeavor remains the successful cultivation of selected species. A historically overlooked but increasingly recognized group of non-model organisms are cave dwellers. These unique animals offer invaluable insights into the genetic basis of human diseases like eye degeneration, metabolic and neurological disorders, and basic evolutionary principles and the origin of adaptive phenotypes. However, to take advantage of the beneficial traits of cave-dwelling animals, laboratory cultures must be established—a practice that remains extremely rare except for the cavefish Astyanax mexicanus. For most cave-dwelling organisms, there are no published culturing protocols. In this study, we present the results of our multi-year effort to establish laboratory cultures for a variety of invertebrate groups. We have developed comprehensive protocols for housing, feeding, and husbandry of cave dwellers and their surface relatives. Our recommendations are versatile and can be applied to a wide range of species. Hopefully our efforts will facilitate the establishment of new laboratory animal facilities for cave-dwelling organisms and encourage their greater use in experimental biology.

Alpioniscus (Illyrionethes) iapodicus n. sp. is described and illustrated. Representing the 14th nominal species of the subgenus Illyrionethes from the Dinaric karst, it is a troglobiotic species collected from the caves in the central...

Alpioniscus (Illyrionethes) is a widespread subgenus of Trichoniscidae in the Dinaric Karst with 15 nominal troglobiotic species. Here we redescribe Alpioniscus (Illyrionethes) magnus and describe three new species (A. lossinii n. sp., A. drazinai n. sp., and A. mandalinae n. sp.) restricted to coastal areas. As a result, 18 nominal Illyrionethes species are known from the Dinaric Karst.