Detection of infectious diseases in wastewater SARS-CoV-2 can be detected in wastewater. The MDEGLE and MDHHS organized multiple institutions across Michigan, to more rapidly detect the circulation of the SARS-CoV-2 virus within specific communities. We also use qPCR, Nested-PCR and sequencing to track the genetic evolution of variants in the wastewater. Future directions could include adapting this protocol for early detection of other viruses and infectious diseases. Microbial-source tracking in recreational water With MDEGLE and the EPA, we are using a standardized qPCR method to detect E. coli in beach water. In addition to E. coli monitoring, we are also emphasizing on using genetic techniques to determine sources of fecal pollution in watersheds. Microbial source tracking, or MST, can detect and quantify fecal pollution originating from various sources. We are currently working with the BEDHD and KCHD to apply this method at beaches across the State of Michigan. Late Embryogenesis Abundant Proteins Gene Family in the Orchidaceae Orchidaceae is increasingly endangered due to anthropogenic disturbances and climate change. The seeds of most species acquire the ability to tolerate extreme drying near the end of development. Maturation drying entails many changes, including the accumulation of LEA proteins and this is necessary for desiccation tolerance. Our research use molecular evidence to show that orchid seeds undergo maturation drying late in development.