The recovery of cathode and anode materials plays an important role in the recycling process of spent lithium-ion batteries (LIBs). Organic binders reduce the liberation efficiency and flotation efficiency of electrode materials derived from spent LIBs. In this study, pyrolysis technology is used to improve the recovery of cathode and anode materials from spent LIBs by removing organic binders. Pyrolysis characteristics of organics in electrode materials are investigated, and on this basis, the effects of pyrolysis parameters on the liberation efficiency of electrode materials are studied. Afterwards, flotation technology is used to separate cathode material from anode material. The results indicate that the optimum liberation efficiency of electrode materials is obtained at a pyrolysis temperature of 500 °C, a pyrolysis time of 15 min and a pyrolysis heating rate of 10 °C/min. At this time, the liberation efficiency of cathode materials is 98.23% and the liberation efficiency of anode materials is 98.89%. Phase characteristics of electrode materials cannot be changed under these pyrolysis conditions. Ultrasonic cleaning was used to remove pyrolytic residues to further improve the flotation efficiency of electrode materials. The cathode material grade was up to 93.89% with a recovery of 96.88% in the flotation process.

Background: Liver metastasis poses a significant barrier to effective immunotherapy in patients with colorectal cancer. Cryoablation has emerged as a vital supplementary therapeutic approach for these patients. However, its impact on the tumor microenvironment following the ablation of liver metastases remains unclear. Methods: We acquired multi-omics time-series data at 1 day, 5 days, and 14 days post-cryoablation, based on tumor and peripheral blood samples from clinical patients, cell co-culture models, and a liver metastases mouse model built on the MC38 cell line in C57BL/6 J mice. This dataset included single-cell transcriptomic sequencing, bulk tissue transcriptomic sequencing, 4D-Label-Free proteomics, flow cytometry data, western blot data, and histological immunofluorescence staining of pathological specimens. Results: We found that a neutrophil-related inflammatory state persisted for at least 14 days post-cryoablation. During this period, neutrophils underwent phenotypic changes, shifting from the N1 to the N2 type. Cryoablation also caused a significant increase in intracellular Ca²⁺ concentration in neutrophils, which triggered the formation of PAD4-dependent neutrophil extracellular traps (NETs), further promoting immune evasion. Moreover, animal studies demonstrated that depleting or inhibiting the CXCL2-CXCR2 signaling axis within neutrophils, or degrading NETs, could effectively restore the host’s anti-tumor immune response. Conclusions: These findings underscore the critical role of neutrophils and their NETs in immune escape following cryoablation. Targeting the CXCL2-CXCR2-Ca²⁺-PAD4 axis could enhance the therapeutic response to PD-1 antibodies, providing a potential strategy to improve treatment outcomes for colorectal cancer with liver metastases. Graphical