Botrytis cinerea (B. cinerea) is one of the necrotrophic pathogens resulting in the heaviest commercial losses in cut rose flowers, and the severity of gray mold disease partly depends on the presence of ethylene during the storage and transport. The effectiveness of nano silver (NS) and salicylic acid (SA) was assessed as a novel control agent in protecting the cut rose flowers against B. cinerea infection and ethylene damages. The efficacy of NS and SA was compared with an inoculated control (CON). A non-treated control (NT) was also used to evaluate the natural infection process. The results indicated that pretreatment with 20 mg L⁻¹ NS significantly reduced B. cinerea growth in rose petals during vase periods. NS effectively suppressed the mRNA levels of ethylene biosynthesis genes (RhACS2, RhACS4, and RhACO1) and the reduction in expression levels of ethylene receptor genes (RhETR1, RhETR2, and RhETR5) and the downstream regulator RhCTR2 in rose petals after B. cinerea inoculation. NS application also decreased the expression of the B. cinerea snod-prot-like 1 (Bcspl1) gene which acts as the virulence factor in cut roses. In NS flowers, the maximum quantum yield of PSII (F_v/F_m) value was higher and the leaf temperature was lower on day 1, suggesting that these factors can be used for detecting B. cinerea infection and water stress in cut rose flowers. Furthermore, NS improved water relations and extended the vase life of cut rose flowers by 3.3 d, compared with that of NT flowers. In contrast, SA had no inhibitive effects on both B. cinerea growth and ethylene response in cut roses. The findings from the present study highlight NS as a promising new candidate for preventing B. cinerea infection and ethylene damages and for improving the postharvest quality of cut roses exported overseas.

Flower senescence varies among cut roses (Rosa hybrida L.), and it is known that the postharvest life of ethylene-sensitive flowers is strongly related to the transcriptional accumulation of ethylene biosynthesis genes, whereas that of ethylene-insensitive flowers is shortened by water stress. These different responses of flowers to hormone and water stresses limit the action of preservatives in inhibiting the postharvest deterioration of cut rose flowers. This study was conducted to investigate the effects of the combined application of antibacterial agents and ethylene biosynthesis and binding inhibitors on the postharvest life and quality of the cut rose cultivars ‘Matador’ (ethylene-sensitive) and ‘Dolcetto’ (ethylene-insensitive). Cut flowers were treated with nano silver (NS), a combination of NS and α-aminoisobutyric acid (NS+A), or a combination of NS+A and 1-methylcyclopropene (NS+AM), and they were subsequently exposed to ethylene for 24 h. Treatment effectiveness was compared with that of control (CON) flowers, which were kept in distilled water and exposed only to ethylene. The results showed that all treatments significantly improved the postharvest quality and vase life of both rose cultivars. However, NS+AM most markedly delayed senescence and prolonged the vase life of cut flowers by 217% in ‘Matador’ and 168.4% in ‘Dolcetto’, compared with those of CON flowers. NS+AM also effectively increased the flower diameter and maintenance time of positive water balance and initial fresh weight by enhancing solution absorption as well as suppressing bacterial growth at the cut stem ends of the cut roses. Furthermore, NS+AM greatly suppressed the ethylene-inducible increase of ethylene biosynthesis genes and the reduction of ethylene receptor genes in petals, which resulted in a reduced flower response to exogenous ethylene in both rose cultivars. These findings show that NS+AM effectively delays flower senescence in both ethylene sensitive and insensitive cultivars by synergistically preventing ethylene response and water stress in cut flowers.

Botrytis cinerea (B. cinerea) causes gray mold disease (GMD), which results in physiological disorders in plants that decrease the longevity and economic value of horticultural crops. To prevent the spread of GMD during distribution, a rapid, early detection technique is necessary. Thermal imaging has been used for GMD detection in various plants, including potted roses; however, its application to cut roses, which have a high global demand, has not been established. In this study, we investigated the utility of thermal imaging for the early detection of B. cinerea infection in cut roses by monitoring changes in petal temperature after fungal inoculation. We examined the effects of GMD on the postharvest quality and petal temperature of cut roses treated with different concentrations of fungal conidial suspensions and chemicals. B. cinerea infection decreased the flower opening, disrupted the water balance, and decreased the vase life of cut roses. Additionally, the average temperature of rose petals was higher for infected flowers than for non-inoculated flowers. One day before the appearance of necrotic symptoms (day 1 of the vase period), the petal temperature in infected flowers was significantly higher, by 1.1 °C, than that of non-inoculated flowers. The GMD-induced increase in petal temperature was associated with the mRNA levels of genes related to ethylene, reactive oxygen species, and water transport. Furthermore, the increase in temperature caused by GMD was strongly correlated with symptom severity and fungal biomass. A multiple regression analysis revealed that the disease incidence in the petals was positively related to the petal temperature one day before the appearance of necrotic symptoms. These results show that thermography is an effective technique for evaluating changes in petal temperature and a possible method for early GMD detection in the cut flower industry.

Post-harvest quality and longevity of cut flowers are critical factors influencing customer satisfaction and purchase decisions. Maintaining post-harvest quality and ensuring a long vase life (VL) present significant challenges to the floriculture industry due to the perishable nature of cut flowers. Recent studies have demonstrated the potential of melatonin (MT), a multifunctional biomolecule, to improve the post-harvest quality and longevity of floricultural products. This review highlights recent advances in the application of MT to improve the longevity and post-harvest quality of cut flowers. The physiological, biochemical, and molecular mechanisms underlying MT’s effects, along with various application methods, are discussed. Furthermore, current challenges and knowledge gaps are identified, and future research directions are proposed to explore MT’s potential in diverse flower species and its practical application in the cut flower industry.

The herbaceous peony (Paeonia lactiflora Pall.) flower is one of the most important ornamental plants in current international flower markets and is widely used for festive occasions because of its bright colors, large flowers, pleasant scent, and plump flower shape. However, the cut peony flowers have a relatively short postharvest life in vases compared to other flower species. The short vase life and susceptibility to gray mold disease caused by Botrytis cinerea significantly influence the commercial value of cut peonies. Here, we reviewed the main factors for postharvest quality reduction in cut peony flowers and the various postharvest treatments aiming for vase life extension and enhancing gray mold disease resistance in cut peonies. Chemical treatments, including ethylene inhibitors, antimicrobial agents, and other preservatives, have been assessed for their effectiveness in improving vase life. Moreover, physical treatments, such as temperature management, controlled atmosphere storage, and dry storage methods, were also discussed for their effectiveness in delaying flower senescence and reducing gray mold disease infection. The review also highlighted the importance of cultivar-specific responses to gray mold disease, ethylene, and water stress, which is important for the development of new effective and specific postharvest practices to improve the vase life of cut peonies.