Skin cancer has always been and remains the leader among all tumors in terms of occurrence. One of the main factors responsible for skin cancer, natural and artificial UV radiation, causes the mutations that transform healthy cells into cancer cells. These mutations inactivate apoptosis, an event required to avoid the malignant transformation of healthy cells. Among these deadliest of cancers, melanoma and its ‘younger sister’, Merkel cell carcinoma, are the most lethal. The heavy toll of skin cancers stems from their rapid progression and the fact that they metastasize easily. Added to this is the difficulty in determining reliable margins when excising tumors and the lack of effective chemotherapy. Possibly the biggest problem posed by skin cancer is reliably detecting the extent to which cancer cells have spread throughout the body. The initial tumor is visible and can be removed, whereas metastases are invisible to the naked eye and much harder to eliminate. In our opinion, antisense oligonucleotides, which can be used in the form of targeted ointments, provide real hope as a treatment that will eliminate cancer cells near the tumor focus both before and after surgery.

Details var a2a_config = a2a_config || {}; a2a_config.onclick = 1; a2a_config.locale = "pl"; a2a_config.color_main = "D7E5ED"; a2a_config.color_border = "9D9D9D"; a2a_config.color_link_text = "ffffff"; a2a_config.color_link_text_hover = "ffffff"; a2a_config.color_bg = "d9d9d9"; a2a_config.icon_color = "#4e4e4e"; a2a_config.exclude_services = ["dihitt", "delicious", "whatsapp", "dzone", "sms", "stumpedia", "viber", "yahoo_messenger", "google_plus"]; Details PDF BIBTEX RIS Title A short history of insecticides Journal title Journal of Plant Protection Research Yearbook 2015 Volume vol. 55 Issue No 3 Authors Oberemok, Volodymyr Volodymyrovych ; Laikova, Kateryna Volodymyrivna ; Gninenko, Yuri Ivanovich ; Zaitsev, Aleksei Sergeevich ; Palmah Mutah Nyadar ; Tajudeen Adesoji Adeyemi Divisions of PAS Nauki Biologiczne i Rolnicze Publisher Committee of Plant Protection PAS ; Institute of Plant Protection – National Research Institute Date 2015[2015.01.01 AD - 2015.12.31 AD] Identifier DOI: 10.1515/jppr-2015-0033 ; ISSN 1427–4345 ; eISSN 1899–007X Source Journal of Plant Protection Research; 2015; vol. 55; No 3 References Inceoglu (2006), Genetically modified baculoviruses : a historical overview and future outlook in, Advances Virus Research, 68, 323, doi.org/10.1016/S0065-3527(06)68009-3 ; Casida (2013), Anticholinesterase insecticide retrospective Chemico - Biological, Interactions, 203, 221. ; Isman (2006), The role of botanical insecticides , deterrents and repellents in modern agriculture and an increasingly regulated world of, Annual Review Entomology, 51, 45, doi.org/10.1146/annurev.ento.51.110104.151146 ; Khalil (2013), Abamectin and azadirachtin as eco - friendly promising biorational tools in integrated nematodes management programs of and, Journal Plant Pathology Microbiology, 4, doi.org/10.4172/2157-7471.1000174 ; Auer (2009), Crop improvement using small RNAs : applications and predictive ecological risk assessments in, Trends Biotechnology, 27, 644, doi.org/10.1016/j.tibtech.2009.08.005 ; De Maagd (2001), HowBacillus thuringiensishas evolved specific toxins to colonize the insect world in, Trends Genetics, 17, 193, doi.org/10.1016/S0168-9525(01)02237-5 ; Wang (2011), Second - generation sequencing supply an effective way to screen RNAi targets in large scale for potential application in pest insect control, PLOS ONE, 6, 18644, doi.org/10.1371/journal.pone.0018644 ; Gordana (2013), The synergy of xenobiotics in honey beeApis mellifera : mechanisms and effects of, Journal Insect Physiology, 58, 613. ; Broughton (2005), The Bhopal disaster and its aftermath : a review, Environmental Health, 4, doi.org/10.1186/1476-069X-4-6 ; Rosell (2008), Biorational insecticides in pest management of, Journal Pesticide Science, 33, 103, doi.org/10.1584/jpestics.R08-01 ; Bate (2007), The rise fall rise and imminent fall of DDT American Enterprise Institute for Public, Policy Research, 14, 1. ; Jeschke (2011), Overview of the status and global strategy for neonicotinoids of and, Journal Agricultural Food Chemistry, 59, 2897, doi.org/10.1021/jf101303g ; Oberemok (2013), a DNA insecticides : application of the iap - gene single - stranded fragments from three different nucleopolyhedroviruses against second instar gypsy moth larvae Universal Journal of, Applied Science, 1, 33. ; Alyokhin (2009), Colorado potato beetle management on potatoes : current challenges and future prospects Fruit Vegetable and Cereal Science and, Biotechnology, 3, 10. ; Kawai (2004), DNA interference : a simple and efficient gene - silencing system for high - throughput functional analysis in the fern Adiantum and, Plant Cell Physiology, 45, 1648, doi.org/10.1093/pcp/pch186 ; Goulson (2013), Review : An overview of the environmental risks posed by neonicotinoid insecticides of, Journal Applied Ecology, 50, 977, doi.org/10.1111/1365-2664.12111 ; Symes (1952), Some recent progress in the study of Insecticides and their application for the control of vectors of disease The of the for the Promotion of, Journal Royal Society Health, 72, 498, doi.org/10.1177/146642405207200505 ; Sanchis (2011), From microbial sprays to insect - resistant transgenic plants : history of the biopesticideBacillus thuringiensis for, Agronomy Sustainable Development, 31, 217, doi.org/10.1051/agro/2010027 ; Gu (2013), Recent advances in RNA interference research in insects : Implications for future insect pest management strategies, Crop Protection, 45, 36, doi.org/10.1016/j.cropro.2012.10.004 ; Nojumian (2015), Sublethal effects of spirodiclofen on life table parameters of minute pirate bugOrius nigerWolff ( Hemiptera : Anthocoridae ) of Entomology and Zoology, Journal Studies, 3, 227. ; Jehle (2006), On the classification and nomenclature of baculoviruses : A proposal for revision of, Archives Virology, 151, 1257, doi.org/10.1007/s00705-006-0763-6 ; Msangi (2011), Field and laboratory evaluation of bioefficacy of an insect growth regulator ( Dimilin ) as a larvicide against mosquito and housefly larvae of Tropical, Journal Medicine, 1. ; Oberemok (2015), DNA insecticides based on iap gene fragments of cabbage looper and gypsy moth nuclear polyhedrosis viruses show selectivity for non - target insects of, Archives Biological Sciences, 37, doi.org/10.2298/ABS1412300370 ; Oberemok (2014), Single - stranded DNA fragments of insect - specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control and, Pesticide Biochemistry Physiology, 113, 1, doi.org/10.1016/j.pestbp.2014.05.005 ; Oberemok (2013), b Pioneer evaluation of the possible side effects of the DNA insecticides on wheat ( Triticum aestivumL of Biochemistry and, International Journal Biophysics, 1, 57. ; Szewczyk (2006), Baculoviruses re - emerging biopesticides, Biotechnology Advances, 24, 143, doi.org/10.1016/j.biotechadv.2005.09.001 ; Oberemok (2008), b Proof of transovarial transmission ofLymantria disparnucleopolyhedrovirus Baculoviridae ) with the RAPD - PCR method Zhurnal Obshchej ( in Russian, Biologii, 69, 397. ; Fire (1998), Potent and specific genetic interference by double - stranded RNA inCaenorhabditis elegans, Nature, 391. ; Chakoosari (2013), Efficacy of various biological and microbial insecticides of Biology and today s world, Journal, 2, 249. ; Oberemok (2015), Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP - gene of, Turkish Journal Biology, 39, 258, doi.org/10.3906/biy-1406-56 ; Miller (1983), Bacterial viral and fungal insecticides, Science, 219. ; Chiu (2012), Insect virus polyhedra , infectious protein crystals that contain virus particles in Structural, Current Opinion Biology, 22, 234, doi.org/10.1016/j.sbi.2012.02.003 ; Weiss (1999), Antisense RNA gene therapy for studying and modulating biological processes Cellular and Molecular Life, Science, 55, 334. ; Sohail (2012), Efficacy of different botanical materials against aphidToxoptera aurantiion tea ( Camellia sinensisL ) cuttings under high shade nursery of Materials and Environmental, Journal Science, 3, 1065. ; Davies (2007), DDT pyrethrins pyrethroids and insect sodium channels, IUBMB Life, 59, 151, doi.org/10.1080/15216540701352042 ; Viktorov (2003), Effect of ivermectin on function of liver macrophages of and, Bulletin Experimental Biology Medicine, 136, 569, doi.org/10.1023/B:BEBM.0000020206.23474.e9 ; Sexton (2007), The economics of pesticides and pest control International Review of Environmental and Resource, Economics, 1, 271. ; Qi (2013), Diamide insecticide target site specificity in the Heliothis and Musca ryanodine receptors relative to toxicity of and, Journal Agricultural Food Chemistry, 62, 4077, doi.org/10.1021/jf501236h ; Majori (2012), Short history of malaria and its eradication in Italy Mediterranean of Hematology and, Journal Infectious Diseases, 4, 16. ; Federici (2010), Overview of the basic biology ofBacillus thuringiensiswith emphasis on genetic engineering of bacterial larvicides for mosquito control Open Toxinology, Journal, 3, 83.

Antisense oligonucleotides (ASO), short single-stranded polymers based on DNA or RNA chemistries and synthesized in vitro, regulate gene expression by binding in a sequence-specific manner to an RNA target. The functional activity and selectivity in the action of ASOs largely depends on the combination of nitrogenous bases in a target sequence. This simple and natural property of nucleic acids provides an attractive route by which scientists can create different ASO-based techniques. Over the last 50 years, planned and realized applications in the field of antisense and nucleic acid nanotechnologies have produced astonishing results and posed new challenges for further developments, exemplifying the essence of the post-genomic era. Today the majority of ASOs are chemically modified and/or incorporated within nanoparticles to enhance their stability and cellular uptake. This review critically analyzes some successful cases using the antisense approach in medicine to address severe diseases, such as Duchenne muscular dystrophy and spinal muscular atrophy, and suggests some prospective directions for future research. We also examine in detail the elaboration of unmodified insect-specific DNA insecticides and RNA preparations in the areas of agriculture and forestry, a relatively new branch of ASO that allows circumvention of the use of non-selective chemical insecticides. When considering the variety of successful ASO modifications with an efficient signal-to-noise ratio of action, coupled with the affordability of in vitro oligonucleotide synthesis and post-synthesis procedures, we predict that the next half-century will produce a fruitful yield of tools created from effective ASO-based end products.

Sternorrhyncha representatives are serious pests of agriculture and forestry all over the world, primarily causing damage to woody plants. Sternorrhyncha members are vectors for the transfer of a large number of viral diseases, and subsequently, the host plant weakens. Additionally, many are inherent in the release of honeydew, on which fungal diseases develop. Today, an innovative approach is needed to create new and effective ways to control the number of these insects based on environmentally friendly insecticides. Of particular relevance to such developments is the need to take into account the large number of organisms living together with insect pests in this group, including beneficial insects. Practically without changing their location on their host plant, they adopted to be more invisible and protected due to their small size, symbiosis with ants, the ability to camouflage with a leaf, and moderately deplete plants and others, rarely leading them to death but still causing substantial economic loss in the subtropics and tropics. Due to the lack of presence in the literature, this review fills in this pesky spot by examining (on the example of distinct species from four superfamilies) the characteristic adaptations for this suborder and the chemical methods of combating these insects that allow them to survive in various environmental conditions, suggesting new and highly promising ways of using olinscides for plant protection against Sternorrhyncha members.

Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus IAP genes can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), and LdMNPV-free larvae, were treated with oligoDNA antisense to the RING (really interesting new gene) domain of the LdMNPV IAP-3 gene. The results with respect to insect mortality, biomass accumulation, histological studies, RT-PCR, and analysis of DNA apoptotic fragmentation suggest that oligoRING induced increased apoptotic processes in both LdMNPV-free and LdMNPV-infected insect cells, but were more pronounced in the latter. These data open up possibilities for promising new routes of insect pest control using antisense phosphodiester DNA oligonucleotides.

Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The current use of chemical insecticides leads to the accumulation of xenobiotics in ecosystems and a decreased number of species in those ecosystems, including insects. Sustainable development of human society is impossible without useful insects, so the control of insect pests must be effective and selective at the same time. In this article, we show for the first time a natural way to regulate the number of insect pests based on the use of extracellular double-stranded DNA secreted by the plant Pittosporum tobira. Using a principle similar to one found in nature, we show that the topical application of artificially synthesized short antisense oligonucleotide insecticides (olinscides, DNA insecticides) is an effective and selective way to control the insect Coccus hesperidum. Using contact oligonucleotide insecticide Coccus-11 at a concentration of 100 ng/μL on C. hesperidum larvae resulted in a mortality of 95.59 ± 1.63% within 12 days. Green oligonucleotide insecticides, created by nature and later discovered by humans, demonstrate a new method to control insect pests that is beneficial and safe for macromolecular insect pest management.