Background: Cancer stem cells’ (CSCs) self-maintenance is regulated via the pluripotency pathways promoting the most aggressive tumor phenotype. This study aimed to use the activity of these pathways for the CSCs’ subpopulation enrichment and separating cells characterized by the OCT4 and SOX2 expression. Methods: To select and analyze CSCs, we used the SORE6x lentiviral reporter plasmid for viral transduction of colon adenocarcinoma cells. Additionally, we assessed cell chemoresistance, clonogenic, invasive and migratory activity and the data of mRNA-seq and intrinsic disorder predisposition protein analysis (IDPPA). Results: We obtained the line of CSC-like cells selected on the basis of the expression of the OCT4 and SOX2 stem cell factors. The enriched CSC-like subpopulation had increased chemoresistance as well as clonogenic and migration activities. The bioinformatic analysis of mRNA seq data identified the up-regulation of pluripotency, development, drug resistance and phototransduction pathways, and the downregulation of pathways related to proliferation, cell cycle, aging, and differentiation. IDPPA indicated that CSC-like cells are predisposed to increased intrinsic protein disorder. Conclusion: The use of the SORE6x reporter construct for CSCs enrichment allows us to obtain CSC-like population that can be used as a model to search for the new prognostic factors and potential therapeutic targets for colon cancer treatment.

Many proteins lack stable 3D structures. These intrinsically disordered proteins (IDPs) or hybrid proteins containing ordered domains with intrinsically disordered protein regions (IDPRs) often carry out regulatory functions related to molecular recognition and signal transduction. IDPs/IDPRs constitute a substantial portion of the human proteome and are termed “the unfoldome”. Herein, we probe the human breast cancer unfoldome and investigate relations between IDPs and key disease genes and pathways. We utilized bottom-up proteomics, MudPIT (Multidimensional Protein Identification Technology), to profile differentially expressed IDPs in human normal (MCF-10A) and breast cancer (BT-549) cell lines. Overall, we identified 2271 protein groups in the unfoldome of normal and cancer proteomes, with 148 IDPs found to be significantly differentially expressed in cancer cells. Further analysis produced annotations of 140 IDPs, which were then classified to GO (Gene Ontology) categories and pathways. In total, 65% (91 of 140) IDPs were related to various diseases, and 20% (28 of 140) mapped to cancer terms. A substantial portion of the differentially expressed IDPs contained disordered regions, confirmed by in silico characterization. Overall, our analyses suggest high levels of interactivity in the human cancer unfoldome and a prevalence of moderately and highly disordered proteins in the network.

Mapping volcanic flow deposits can be achieved by considering backscattering characteristics as a metric of surface roughness. In this study, we developed an approach to extract a measure of surface roughness from dual-band airborne Synthetic Aperture Radar (ASAR) backscattering data to characterize and map various volcanic flow deposits—namely, debris avalanches, lahars, lava flows, and pyroclastic density currents. We employed ASAR and Indian Space Research Organization (ISRO) airborne SAR datasets, from a joint project (ASAR-ISRO), acquired in December 2019 at 2 m spatial resolution, to assess the role and importance of incorporating dual-band data, i.e., L-band and S-band, into surface roughness models. Additionally, we derived and analyzed surface roughness from a digital surface model (DSM) generated from unoccupied aircraft systems (UAS) acquisitions using Structure from Motion (SfM) photogrammetry techniques. These UAS-derived surface roughness outputs served as meter-scale calibration products to validate the radar roughness data over targeted areas. Herein, we applied our method to a region in the United States over the Mount St. Helens volcano in the Cascade Range of Washington state. Our results showed that dual-band systems can be utilized to characterize different types of volcanic deposits and range of terrain roughness. Importantly, we found that a combination of radar wavelengths (i.e., 9 and 24 cm), in tandem with high-spatial-resolution backscatter measurements, yields improved surface roughness maps, compared to single-band, satellite-based approaches at coarser resolution. The L-band (24 cm) can effectively differentiate small, medium, and large-scale structures, namely, blocks/boulders from fine-grained lahar deposits and hummocks from debris avalanche deposits. Additionally, variation in the roughness estimates of lahar and debris avalanche deposits can be identified and quantified individually. In contrast, the S-band (9 cm) can distinguish different soil moisture conditions across variable terrain; for example, identify wet active channels. In principle, this dual-band approach can also be employed with time series of various other SAR data of higher coherence (such as satellite SAR), using different wavelengths and polarizations, encompassing a wider range of surface roughness, and ultimately enabling additional applications at other volcanoes worldwide and even beyond volcanology.