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Aflatoxins (AFs) have always been regarded as the most effective carcinogens, posing a great threat to agriculture, food safety, and human health. Aspergillus flavus is the major producer of aflatoxin contamination in crops. The prevention and control of A. flavus and aflatoxin continues to be a global problem. In this study, we demonstrated that the cell-free culture filtrate of Aspergillus oryzae and a non-aflatoxigenic A. flavus can effectively inhibit the production of AFB1 and the growth and reproduction of A. flavus, indicating that both of the non-aflatoxigenic Aspergillus strains secrete inhibitory compounds. Further transcriptome sequencing was performed to analyze the inhibitory mechanism of A. flavus treated with fermenting cultures, and the results revealed that genes involved in the AF biosynthesis pathway and other biosynthetic gene clusters were significantly downregulated, which might be caused by the reduced expression of specific regulators, such as AflS, FarB, and MtfA. The WGCNA results further revealed that genes involved in the TCA cycle and glycolysis were potentially involved in aflatoxin biosynthesis. Our comparative transcriptomics also revealed that two conidia transcriptional factors, brlA and abaA, were found to be significantly downregulated, which might lead to the downregulation of conidiation-specific genes, such as the conidial hydrophobins genes rodA and rodB. In summary, our research provides new insights for the molecular mechanism of controlling AF synthesis to control the proliferation of A. flavus and AF pollution.
Kunlong Yang; Qingru Geng; Fengqin Song; Xiaona He; Tianran Hu; Shihua Wang; Jun Tian. Transcriptome Sequencing Revealed an Inhibitory Mechanism of Aspergillus flavus Asexual Development and Aflatoxin Metabolism by Soy-Fermenting Non-Aflatoxigenic Aspergillus. International Journal of Molecular Sciences 2020, 21, 6994 .
AMA StyleKunlong Yang, Qingru Geng, Fengqin Song, Xiaona He, Tianran Hu, Shihua Wang, Jun Tian. Transcriptome Sequencing Revealed an Inhibitory Mechanism of Aspergillus flavus Asexual Development and Aflatoxin Metabolism by Soy-Fermenting Non-Aflatoxigenic Aspergillus. International Journal of Molecular Sciences. 2020; 21 (19):6994.
Chicago/Turabian StyleKunlong Yang; Qingru Geng; Fengqin Song; Xiaona He; Tianran Hu; Shihua Wang; Jun Tian. 2020. "Transcriptome Sequencing Revealed an Inhibitory Mechanism of Aspergillus flavus Asexual Development and Aflatoxin Metabolism by Soy-Fermenting Non-Aflatoxigenic Aspergillus." International Journal of Molecular Sciences 21, no. 19: 6994.
Ceratocystis fimbriata is the most devastating phytopathogen causing significant losses in post-harvest sweet potato. In this study, monoterpene nerol (NEL), the active compound in neroli essential oil, was found to dose-dependently inhibit the mycelial growth and spore germination of C. fimbriata at a minimum inhibitory concentration (MIC) of 0.25 mL L-1. NEL vapor treatments significantly reduced the incidence and lesion diameter of black rot in sweet potato infected by the fungus and regulated the defense-related enzyme activity of phenylalanine ammonia lyase (PAL). Using RNA sequencing (RNA-seq) and biochemical assays, it was demonstrated that NEL treatment impaired cell membrane integrity via down-regulating the expression of ergosterol synthesis genes and reduced the ergosterol content. Moreover, an analysis of a series of apoptotic events revealed that NEL treatment caused mitochondrial membrane damage by reducing the mitochondrial membrane potential (MMP, Δψm), which led to down-regulation of genes involved in ATP production, then induced accumulation of intracellular reactive oxygen species (ROS) generation. Simultaneously, NEL caused nuclear chromatin condensation and concomitant DNA cleavage, which led to the up-regulation of DNA repair genes expression, and the cell-cycle arrest principally occurred at the G2/M phase in C. fimbriata. Altogether, these findings provide information about the underlying antifungal mechanism of NEL against C. fimbriata and suggest that NEL could be a useful alternative for controlling C. fimbriata in post-harvest spoilage of sweet potato.
Xuezhi Li; Man Liu; Tinggong Huang; Kunlong Yang; Sihan Zhou; Yongxin Li; Jun Tian. Antifungal effect of nerol via transcriptome analysis and cell growth repression in sweet potato spoilage fungi Ceratocystis fimbriata. Postharvest Biology and Technology 2020, 171, 111343 .
AMA StyleXuezhi Li, Man Liu, Tinggong Huang, Kunlong Yang, Sihan Zhou, Yongxin Li, Jun Tian. Antifungal effect of nerol via transcriptome analysis and cell growth repression in sweet potato spoilage fungi Ceratocystis fimbriata. Postharvest Biology and Technology. 2020; 171 ():111343.
Chicago/Turabian StyleXuezhi Li; Man Liu; Tinggong Huang; Kunlong Yang; Sihan Zhou; Yongxin Li; Jun Tian. 2020. "Antifungal effect of nerol via transcriptome analysis and cell growth repression in sweet potato spoilage fungi Ceratocystis fimbriata." Postharvest Biology and Technology 171, no. : 111343.
Ochratoxin A (OTA) is a ubiquitous food contaminant and a critical food safety concern due to its nephron toxic effects, which impacts all parts of the world. Luteolin (LUT) had gained increasing interest as a health-promoting food antioxidant component. However, the preventative effect of LUT against OTA induced oxidative stress was not yet clear and the elucidation of which would provide critical information to develop dietary LUT as a control strategy for OTA. In the current study, the cytoprotective effect of LUT against OTA induced oxidative stress and the mechanism(s) behind was examined in NRK-52E rat kidney cells. The results showed that LUT exerted its preventative effect via restoring cell viability and preventing LDH release. It alleviated the OTA-induced oxidative stress and lipid peroxidation by reducing ROS accumulation, ameliorating the mitochondrial membrane potential reduction and reversing the activities of antioxidant enzymes to the control levels. The regulating roles of Nrf2 and HIF-1α in this process were evaluated by cell immunofluorescence assay, reporter plasmids transfection assay and qRT-PCR analysis. The results showed that LUT activated Nrf2 pathway and increased the antioxidant defense capacities of OTA treated cells. Additionally, LUT also modulated HIF-1α pathway to initiate the angiogenesis and epithelial restitution process.
Man Liu; Chao Cheng; Xuezhi Li; Sihan Zhou; Jiali Hua; Jie Huang; Yongxin Li; Kunlong Yang; Peng Zhang; Yan Zhang; Jun Tian. Luteolin alleviates ochratoxin A induced oxidative stress by regulating Nrf2 and HIF-1α pathways in NRK-52E rat kidney cells. Food and Chemical Toxicology 2020, 141, 111436 .
AMA StyleMan Liu, Chao Cheng, Xuezhi Li, Sihan Zhou, Jiali Hua, Jie Huang, Yongxin Li, Kunlong Yang, Peng Zhang, Yan Zhang, Jun Tian. Luteolin alleviates ochratoxin A induced oxidative stress by regulating Nrf2 and HIF-1α pathways in NRK-52E rat kidney cells. Food and Chemical Toxicology. 2020; 141 ():111436.
Chicago/Turabian StyleMan Liu; Chao Cheng; Xuezhi Li; Sihan Zhou; Jiali Hua; Jie Huang; Yongxin Li; Kunlong Yang; Peng Zhang; Yan Zhang; Jun Tian. 2020. "Luteolin alleviates ochratoxin A induced oxidative stress by regulating Nrf2 and HIF-1α pathways in NRK-52E rat kidney cells." Food and Chemical Toxicology 141, no. : 111436.
Perillaldehyde (PAE), an essential oil in Perilla plants, serves as a safe flavor ingredient in foods, and shows an effectively antifungal activity. Reactive oxygen species (ROS) accumulation in Aspergillus flavus plays a critical role in initiating a metacaspase-dependent apoptosis. However, the reason for ROS accumulation in A. flavus is not yet clear. Using transcriptome sequencing of A. flavus treated with different concentrations of PAE, our data showed that the ROS accumulation might have been as a result of an inhibition of energy metabolism with less production of reducing power. By means of GO and KEGG enrichment analysis, we screened four key pathways, which were divided into two distinct groups: a downregulated group that was made up of the glycolysis and pentose phosphate pathway, and an upregulated group that consisted of MAPK signaling pathway and GSH metabolism pathway. The inhibition of dehydrogenase gene expression in two glycometabolism pathways might play a crucial role in antifungal mechanism of PAE. Also, in our present study, we systematically showed a gene interaction network of how genes of four subsets are effected by PAE stress on glycometabolism, oxidant damage repair, and cell cycle control. This research may contribute to explaining an intrinsic antifungal mechanism of PAE against A. flavus.
Chao Pan; Yong-Xin Li; Kunlong Yang; Erhunmwunsee Famous; Yan Ma; Xiaona He; Qingru Geng; Man Liu; Jun Tian. The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing. International Journal of Molecular Sciences 2020, 21, 1518 .
AMA StyleChao Pan, Yong-Xin Li, Kunlong Yang, Erhunmwunsee Famous, Yan Ma, Xiaona He, Qingru Geng, Man Liu, Jun Tian. The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing. International Journal of Molecular Sciences. 2020; 21 (4):1518.
Chicago/Turabian StyleChao Pan; Yong-Xin Li; Kunlong Yang; Erhunmwunsee Famous; Yan Ma; Xiaona He; Qingru Geng; Man Liu; Jun Tian. 2020. "The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing." International Journal of Molecular Sciences 21, no. 4: 1518.
Aspergillus flavus is one of the most opportunistic pathogens invading many important oilseed crops and foodstuffs with such toxic secondary metabolites as aflatoxin (AF) and Cyclopiazonic acid. We previously used the DNA methylation inhibitor 5-azacytidine to treat with an AF-producing A. flavus A133 strain, and isolated a mutant (NT) of A. flavus, which displayed impaired abilities of AF biosynthesis and fungal development. In this study, gas chromatography–mass spectrometry (GC-MS) analysis was used to reveal the metabolic changes between these two strains. A total of 1181 volatiles were identified in these two strains, among which 490 volatiles were found in these two strains in vitro and 332 volatiles were found in vivo. The NT mutant was found to produce decreasing volatile compounds, among which most of the fatty acid-derived volatiles were significantly downregulated in the NT mutant compared to the A133 strain, which are important precursors for AF biosynthesis. Two antioxidants and most of the amino acids derived volatiles were found significantly upregulated in the NT mutant. Overall, our results reveal the difference of metabolic profiles in two different A. flavus isolates, which may provide valuable information for controlling infections of this fungal pathogen.
Fengqin Song; Qingru Geng; Xuewei Wang; Xiaoqing Gao; Xiaona He; Wei Zhao; Huahui Lan; Jun Tian; Kunlong Yang; Shihua Wang. Gas Chromatography–Mass Spectrometry Profiling of Volatile Compounds Reveals Metabolic Changes in a Non-Aflatoxigenic Aspergillus flavus Induced by 5-Azacytidine. Toxins 2020, 12, 57 .
AMA StyleFengqin Song, Qingru Geng, Xuewei Wang, Xiaoqing Gao, Xiaona He, Wei Zhao, Huahui Lan, Jun Tian, Kunlong Yang, Shihua Wang. Gas Chromatography–Mass Spectrometry Profiling of Volatile Compounds Reveals Metabolic Changes in a Non-Aflatoxigenic Aspergillus flavus Induced by 5-Azacytidine. Toxins. 2020; 12 (1):57.
Chicago/Turabian StyleFengqin Song; Qingru Geng; Xuewei Wang; Xiaoqing Gao; Xiaona He; Wei Zhao; Huahui Lan; Jun Tian; Kunlong Yang; Shihua Wang. 2020. "Gas Chromatography–Mass Spectrometry Profiling of Volatile Compounds Reveals Metabolic Changes in a Non-Aflatoxigenic Aspergillus flavus Induced by 5-Azacytidine." Toxins 12, no. 1: 57.
Aspergillus flavus is a renowned plant, animal and human pathogen. areA is a global nitrogen regulatory gene of the GATA transcription factor family, shown to be the major nitrogen regulator. In this study, we identified areA in A. flavus and studied its function. The AreA protein contained a signatory zinc finger domain, which is extremely conserved across fungal species. Gene deletion (ΔareA) and over-expression (OE::areA) strains were constructed by homologous recombination to elucidate the role of areA in A. flavus. The ΔareA strain was unable to efficiently utilize secondary nitrogen sources for growth of A. flavus, and it had poorly developed conidiophores, when observed on complete medium, resulting in the production of significantly less conidia than the wild-type strain (WT). Aflatoxin B1 (AFB1) production was reduced in ΔareA compared with the WT strain in most conditions tested, and ΔareA had impaired virulence in peanut seeds. areA also played important roles in the sensitivity of A. flavus to osmotic, cell wall and oxidative stresses. Hence, areA was found to be important for the growth, aflatoxin production and pathogenicity of A. flavus. This work sheds light on the function of areA in the regulation of the nitrogen metabolism of A. flavus, and consequently aims at providing new ways for controlling the crossover pathogen, A. flavus.
Opemipo Esther Fasoyin; Kunlong Yang; Mengguang Qiu; Bin Wang; Sen Wang; Shihua Wang. Regulation of Morphology, Aflatoxin Production, and Virulence of Aspergillus flavus by the Major Nitrogen Regulatory Gene areA. Toxins 2019, 11, 718 .
AMA StyleOpemipo Esther Fasoyin, Kunlong Yang, Mengguang Qiu, Bin Wang, Sen Wang, Shihua Wang. Regulation of Morphology, Aflatoxin Production, and Virulence of Aspergillus flavus by the Major Nitrogen Regulatory Gene areA. Toxins. 2019; 11 (12):718.
Chicago/Turabian StyleOpemipo Esther Fasoyin; Kunlong Yang; Mengguang Qiu; Bin Wang; Sen Wang; Shihua Wang. 2019. "Regulation of Morphology, Aflatoxin Production, and Virulence of Aspergillus flavus by the Major Nitrogen Regulatory Gene areA." Toxins 11, no. 12: 718.
The fundamental biological function of nucleoside diphosphate kinase (NDK) is to catalyze the reversible exchange of the γ-phosphate between nucleoside triphosphate (NTP) and nucleoside diphosphate (NDP). This kinase also has functions that extend beyond its canonically defined enzymatic role as a phosphotransferase. However, the role of NDK in filamentous fungi, especially in Aspergillus flavus (A. flavus), is not yet known. Here we report that A. flavus has two NDK-encoding gene copies as assessed by qPCR. Using gene-knockout and complementation experiments, we found that AfNDK regulates spore and sclerotia development and is involved in plant virulence as assessed in corn and peanut seed-based assays. An antifungal test with the inhibitor azidothymidine suppressed AfNDK activity in vitro and prevented spore production and sclerotia formation in A. flavus, confirming AfNDK's regulatory functions. Crystallographic analysis of AfNDK, coupled with site-directed mutagenesis experiments, revealed three residues (Arg-104, His-117, and Asp-120) as key sites that contribute to spore and sclerotia development. These results not only enrich our knowledge of the regulatory role of this important protein in A. flavus, but also provide insights into the prevention of A. flavus infection in plants and seeds, as well as into the structural features relevant for future antifungal drug development.
Yu Wang; Sen Wang; Xinyi Nie; Kunlong Yang; Peng Xu; Xiuna Wang; Mengxin Liu; Yongshuai Yang; Zhuo Chen; Shihua Wang. Molecular and structural basis of nucleoside diphosphate kinase–mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus. Journal of Biological Chemistry 2019, 294, 12415 -12431.
AMA StyleYu Wang, Sen Wang, Xinyi Nie, Kunlong Yang, Peng Xu, Xiuna Wang, Mengxin Liu, Yongshuai Yang, Zhuo Chen, Shihua Wang. Molecular and structural basis of nucleoside diphosphate kinase–mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus. Journal of Biological Chemistry. 2019; 294 (33):12415-12431.
Chicago/Turabian StyleYu Wang; Sen Wang; Xinyi Nie; Kunlong Yang; Peng Xu; Xiuna Wang; Mengxin Liu; Yongshuai Yang; Zhuo Chen; Shihua Wang. 2019. "Molecular and structural basis of nucleoside diphosphate kinase–mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus." Journal of Biological Chemistry 294, no. 33: 12415-12431.
Aspergillus flavus, a ubiquitous filamentous fungus found in soil, plants and other substrates has been reported not only as a pathogen for plants, but also a carcinogen producing fungus for human. Peptidyl-Prolyl Isomerase (PPIases) plays an important role in cell process such as protein secretion cell cycle control and RNA processing. However, the function of PPIase has not yet been identified in A. flavus. In this study, the PPIases gene from A. flavus named ppci1 was cloned into expression vector and the protein was expressed in prokaryotic expression system. Activity of recombinant ppci1 protein was particularly inhibited by FK506, CsA and rapamycin. 3D-Homology model of ppci1 has been constructed with the template, based on 59.7% amino acid similarity. The homologous recombination method was used to construct the single ppci1 gene deletion strain Δppci1. We found that, the ppci1 gene plays important roles in A. flavus growth, conidiation, and sclerotia formation, all of which showed reduction in Δppci1 and increased in conidiation compared with the wild-type and complementary strains in A. flavus. Furthermore, aflatoxin and peanut seeds infection assays indicated that ppci1 contributes to virulence of A. flavus. Furthermore, we evaluated the effect of PPIase inhibitors on A. flavus growth, whereby these were used to treat wild-type strains. We found that the growths were inhibited under every inhibitor. All, these results may provide valuable information for designing inhibitors in the controlling infections of A. flavus.
Saleem Ahmad; Sen Wang; Weizhong Wu; Kunlong Yang; Yan Feng Zhang; Elisabeth Tumukunde; Shihua Wang; Yu Wang. Functional Analysis of Peptidyl-prolyl cis-trans Isomerase from Aspergillus flavus. International Journal of Molecular Sciences 2019, 20, 2206 .
AMA StyleSaleem Ahmad, Sen Wang, Weizhong Wu, Kunlong Yang, Yan Feng Zhang, Elisabeth Tumukunde, Shihua Wang, Yu Wang. Functional Analysis of Peptidyl-prolyl cis-trans Isomerase from Aspergillus flavus. International Journal of Molecular Sciences. 2019; 20 (9):2206.
Chicago/Turabian StyleSaleem Ahmad; Sen Wang; Weizhong Wu; Kunlong Yang; Yan Feng Zhang; Elisabeth Tumukunde; Shihua Wang; Yu Wang. 2019. "Functional Analysis of Peptidyl-prolyl cis-trans Isomerase from Aspergillus flavus." International Journal of Molecular Sciences 20, no. 9: 2206.
In Aspergillus, the cyclic AMP signaling modulates asexual development and mycotoxin biosynthesis. Here, we characterize the cyclase-associated protein Cap in the pathogenic fungus Aspergillus flauvs. The cap disruption mutant exhibited dramatic reduction in hyphal growth, conidiation and spore germination. While an enhance production of the sclerotia was observed in this mutant. Importantly, the cap gene was found important for mycotoxins biosynthesis and virulence. The domain deletion study demonstrated that each domain played important roles for Cap protein in regulating cAMP/PKA signaling, while only P1 and CARP domain were essential for the full function of Cap. The phosphorylation of Cap at S35 was identified in A. flavus, which was found to play a negligible role for the function of Cap. Overall, our results indicated that Cap with multi-domain engages in mycotoxins production and fungal pathogenicity, which could be designed as potential control targets for preventing this fungal pathogen.
Kunlong Yang; Yinghang Liu; Sen Wang; Lianghuan Wu; Rui Xie; Huahui Lan; Opemipo Esther Fasoyin; Yu Wang; Shihua Wang. Cyclase-Associated Protein Cap with Multiple Domains Contributes to Mycotoxin Biosynthesis and Fungal Virulence in Aspergillus flavus. Journal of Agricultural and Food Chemistry 2019, 67, 4200 -4213.
AMA StyleKunlong Yang, Yinghang Liu, Sen Wang, Lianghuan Wu, Rui Xie, Huahui Lan, Opemipo Esther Fasoyin, Yu Wang, Shihua Wang. Cyclase-Associated Protein Cap with Multiple Domains Contributes to Mycotoxin Biosynthesis and Fungal Virulence in Aspergillus flavus. Journal of Agricultural and Food Chemistry. 2019; 67 (15):4200-4213.
Chicago/Turabian StyleKunlong Yang; Yinghang Liu; Sen Wang; Lianghuan Wu; Rui Xie; Huahui Lan; Opemipo Esther Fasoyin; Yu Wang; Shihua Wang. 2019. "Cyclase-Associated Protein Cap with Multiple Domains Contributes to Mycotoxin Biosynthesis and Fungal Virulence in Aspergillus flavus." Journal of Agricultural and Food Chemistry 67, no. 15: 4200-4213.
The heterotrimeric G proteins are critical for signal transduction and function in numerous biological processes including vegetative growth, asexual development and fungal virulence in fungi. Here, we identified four G protein alpha subunits (GanA, GpaB, FadA and GaoC) in the notorious Aflatoxin-producing fungus Aspergillus flavus. GanA, GpaB and FadA have homologues in other fungal species, while GaoC is a novel one. Here, we showed that the loss function of gpaB displayed a defect in conidiophore formation and considerably reduced expression levels of conidia-specific genes brlA and abaA. A decreased viability of cell wall integrity stress and oxidative stress were also found in the ∆gpaB mutant. More importantly, aflatoxin (AF) biosynthesis and infection on crop seeds were severely impaired in the gpaB-deficient mutant. Further analyses demonstrated that the intracellular cAMP levels significantly reduced in the gpaB-deficient mutant compared to wildtype strains. Additionally, an alteration of PKA activities in the ∆gpaB mutant was also found. Overall, our results indicated that GpaB played diverse roles in asexual sporulation, AF biosynthesis and virulence by regulating cAMP signaling in Aspergillus flavus.
Yinghang Liu; Kunlong Yang; Qiuping Qin; Guinan Lin; Tianran Hu; Zhangling Xu; Shihua Wang. G Protein α Subunit GpaB is Required for Asexual Development, Aflatoxin Biosynthesis and Pathogenicity by Regulating cAMP Signaling in Aspergillus flavus. Toxins 2018, 10, 117 .
AMA StyleYinghang Liu, Kunlong Yang, Qiuping Qin, Guinan Lin, Tianran Hu, Zhangling Xu, Shihua Wang. G Protein α Subunit GpaB is Required for Asexual Development, Aflatoxin Biosynthesis and Pathogenicity by Regulating cAMP Signaling in Aspergillus flavus. Toxins. 2018; 10 (3):117.
Chicago/Turabian StyleYinghang Liu; Kunlong Yang; Qiuping Qin; Guinan Lin; Tianran Hu; Zhangling Xu; Shihua Wang. 2018. "G Protein α Subunit GpaB is Required for Asexual Development, Aflatoxin Biosynthesis and Pathogenicity by Regulating cAMP Signaling in Aspergillus flavus." Toxins 10, no. 3: 117.
Aspergillus flavus is a common fungal pathogen of plants, animals and humans. Recently, many genes of A. flavus have been reported involving in regulation of pathogenesis in crops, but whether these genes are involved in animal virulence is still unknown. Here, we used a previous easy-to-use infection model for A. flavus based on mouse model by intravenous inoculation of A. flavus conidia. The outcome of infections in mice model showed that A. flavus NRRL3357 and laboratory strain CA14 PTS were both in dose dependent manner and highly reproducible. The progress of disease could be monitored by mice survival and histology analysis. Fungal burden analysis indicated it was gradually decreased within 7 days after infection. Moreover, aspergillosis caused by A. flavus significantly up-regulated gene expression levels of immune response mediators, including INF-γ, TNF-α, Dectin-1 and TLR2. Furthermore, the defined deletion A. flavus strains that previously displayed virulence in crop infection were also determined in this mouse model, and the results showed comparable degrees of infection in mice. Our results suggested that intravenous inoculation of conidia could be a suitable model for testing different A. flavus mutants in animal virulence. We hope to use this model to determine distinct A. flavus strains virulence in animals and study novel therapeutic methods to help control fungus diseases in the future.
Huahui Lan; Lianghuan Wu; Ruilin Sun; Kunlong Yang; Yinghang Liu; Jiefei Wu; Longpo Geng; Chuanzhong Huang; Shihua Wang. Investigation of Aspergillus flavus in animal virulence. Toxicon 2018, 145, 40 -47.
AMA StyleHuahui Lan, Lianghuan Wu, Ruilin Sun, Kunlong Yang, Yinghang Liu, Jiefei Wu, Longpo Geng, Chuanzhong Huang, Shihua Wang. Investigation of Aspergillus flavus in animal virulence. Toxicon. 2018; 145 ():40-47.
Chicago/Turabian StyleHuahui Lan; Lianghuan Wu; Ruilin Sun; Kunlong Yang; Yinghang Liu; Jiefei Wu; Longpo Geng; Chuanzhong Huang; Shihua Wang. 2018. "Investigation of Aspergillus flavus in animal virulence." Toxicon 145, no. : 40-47.
Lysine methyltransferases transfer methyl groups in specific lysine sites, which regulates a variety of important biological processes in eukaryotes. In this study, we characterized a novel homolog of the yeast methyltransferase DOT1 in A. flavus, and observed the roles of dot1 in A. flavus. Deletion of dot1 showed a significant decrease in conidiation, but an increase in sclerotia formation. A change in viability to multiple stresses was also found in the Δdot1 mutant. Additionally, aflatoxin (AF) production was found severely impaired in the Δdot1 mutant. Further analysis by qRT-PCR revealed that the transcription of AF structural genes and their regulator gene aflS were prominently suppressed in the Δdot1 mutant. Furthermore, our data revealed that Dot1 is important for colonizing maize seeds in A. flavus. Our research indicates that Dot1 is involved in fungal development, aflatoxin biosynthesis and fungal virulence in A. flavus, which might provide a potential target for controlling A. flavus with new strategies.
Linlin Liang; Yinghang Liu; Kunlong Yang; Guinan Lin; Zhangling Xu; Huahui Lan; Xiuna Wang; Shihua Wang. The Putative Histone Methyltransferase DOT1 Regulates Aflatoxin and Pathogenicity Attributes in Aspergillus flavus. Toxins 2017, 9, 232 .
AMA StyleLinlin Liang, Yinghang Liu, Kunlong Yang, Guinan Lin, Zhangling Xu, Huahui Lan, Xiuna Wang, Shihua Wang. The Putative Histone Methyltransferase DOT1 Regulates Aflatoxin and Pathogenicity Attributes in Aspergillus flavus. Toxins. 2017; 9 (7):232.
Chicago/Turabian StyleLinlin Liang; Yinghang Liu; Kunlong Yang; Guinan Lin; Zhangling Xu; Huahui Lan; Xiuna Wang; Shihua Wang. 2017. "The Putative Histone Methyltransferase DOT1 Regulates Aflatoxin and Pathogenicity Attributes in Aspergillus flavus." Toxins 9, no. 7: 232.