The development of UAVs and multispectral cameras has led to remote sensing applications with unprecedented spatial resolution. However, uncertainty remains on the radiometric calibration process for converting raw images to surface reflectance. Several calibration methods exist, but the advantages and disadvantages of each are not well understood. We performed an empirical analysis of five different methods for calibrating a 10-band multispectral camera, the MicaSense RedEdge MX Dual Camera System, by comparing multispectral images with spectrometer measurements taken in the field on the same day. Two datasets were collected, one in clear-sky and one in overcast conditions on the same field. We found that the empirical line method (ELM), using multiple radiometric reference targets imaged at mission altitude performed best in terms of bias and RMSE. However, two user-friendly commercial solutions relying on one single grey reference panel were only slightly less accurate and resulted in sufficiently accurate reflectance maps for most applications, particularly in clear-sky conditions. In overcast conditions, the increase in accuracy of more elaborate methods was higher. Incorporating measurements of an integrated downwelling light sensor (DLS2) did not improve the bias nor RMSE, even in overcast conditions. Ultimately, the choice of the calibration method depends on required accuracy, time constraints and flight conditions. When the more accurate ELM is not possible, commercial, user-friendly solutions like the ones offered by Agisoft Metashape and Pix4D can be good enough.

Alternaria solani is the second most devastating foliar pathogen of potato crops worldwide, causing premature defoliation of the plants. This disease is currently prevented through the regular application of detrimental crop protection products and is guided by early warnings based on weather predictions and visual observations by farmers. To reduce the use of crop protection products, without additional production losses, it would be beneficial to be able to automatically detect Alternaria solani in potato fields. In recent years, the potential of deep learning in precision agriculture is receiving increasing research attention. Convolutional Neural Networks (CNNs) are currently the state of the art, but also come with challenges, especially regarding in-field robustness. This stems from the fact that they are often trained on datasets that are limited in size or have been recorded in controlled environments, not necessarily representative of real-world settings. We collected a dataset consisting of ultra-high-resolution modified RGB UAV-imagery of both symptomatic and non-symptomatic potato crops in the field during various years and disease stages to cover the great variability in agricultural data. We developed a convolutional neural network to perform in-field detection of Alternaria, defined as a binary classification problem. Our model achieves a similar accuracy as several state-of-the-art models for disease detection, but has a much lower inference time, which enhances its practical applicability. By using training data of three consecutive growing seasons (2019, 2020 and 2021) and test data of an independent fourth year (2022), an F1 score of 0.93 is achieved. Furthermore, we evaluate how different properties of the dataset such as its size and class imbalance impact the obtained accuracy.