I obtained my PhD in Molecular Medicine from Sapienza University of Rome and currently hold a Researcher position in the Department of Experimental Medicine. My research focuses on the development and validation of multi-analyte liquid biopsy approaches for precision oncology, with particular interest in circulating tumor cells (CTCs), circulating hybrid cells (CHCs), extracellular vesicles (EVs), and circulating tumor DNA (ctDNA). My work includes the molecular and functional characterization of CTCs, the development of 2D and 3D co-culture models, and the analysis of plasma- and urine-derived ctDNA for non-invasive molecular profiling in colorectal and bladder cancer. I have also contributed to the development and validation of automated image analysis tools for the detection and characterization of CTCs, CHCs, EVs, and cancer-associated macrophage-like cells (CAMLs). From October 2024 to March 2025, I carried out part of my PhD research at the National Center for Tumor Diseases (NCT)/German Cancer Research Center (DKFZ) in Heidelberg, Germany, where I developed 3D tumor–fibroblast co-culture models to investigate the molecular mechanisms underlying the neo-RAS wild-type phenotype in metastatic colorectal cancer. My current research focuses on improving molecular characterization strategies based on CTC isolation and downstream DNA analysis.