Abiotic stresses are one of the significant threats to soybean (Glycine max L.) growth and yields worldwide. Soybean has a crucial role in the global food supply chain and food security and contributes the main protein share compared to other crops. Hence, there is a vast scientific saddle on soybean researchers to develop tolerant genotypes to meet the growing need of food for the huge population. A large portion of cultivated land is damaged by salinity stress, and the situation worsens yearly. In past years, many attempts have increased soybean resilience to salinity stress. Different molecular techniques such as quantitative trait loci mapping (QTL), genetic engineering, transcriptome, transcription factor analysis (TFs), CRISPR/Cas9, as well as other conventional methods are used for the breeding of salt-tolerant cultivars of soybean to safeguard its yield under changing environments. These powerful genetic tools ensure sustainable soybean yields, preserving genetic variability for future use. Only a few reports about a detailed overview of soybean salinity tolerance have been published. Therefore, this review focuses on a detailed overview of several molecular techniques for soybean salinity tolerance and draws a future research direction. Thus, the updated review will provide complete guidelines for researchers working on the genetic mechanism of salinity tolerance in soybean.

As a most significant cereal crop, maize provides vital nutritional components to humans and livestock. Drought stress curtails maize growth and yield by impairing several morphological, physiological, and biochemical functions. The rising threats of drought stress significantly affect global food security and increase the ratio of hunger and starvation. The use of molecular breeding techniques has enabled maize researchers to deeply examine the genetic control of drought tolerance and the genetic differences between genotypes to drought stress. Despite the significant progress in molecular genetics, the drought tolerance mechanism is still not fully understood. With the advancements in molecular research, researchers have identified several molecular factors associated with maize tolerance to drought stress. Quantitative trait loci (QTL) mapping and genome-wide association study (GWAS) analysis have led to identifying QTL, and genes linked to drought tolerance in maize that can be further exploited for their possible breeding applications. Transcriptome and transcription factors (TFs) analysis has revealed the documentation of potential genes and protein groups that might be linked to drought tolerance and accelerate the drought breeding program. Genetic engineering has been used to develop transgenic maize cultivars that are resistant to drought stress. Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) is a new ray of hope to edit the gene of interest to enhance drought tolerance in maize and save both time and cost in cultivar development. In the current review article, we have tried to present an updated picture of the advancements of drought tolerance in maize and its future prospects. These organized pieces of information can assist future researchers in understanding the basis of drought tolerance to adopt a potential breeding tool for breeding drought-tolerant maize cultivars.

Ramie cell walls play an important role in cadmium (Cd) detoxification. However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear. Therefore, this study compared two ramie populations (‘Dazhuhuangbaima’ (low-Cd-accumulating population) and ‘Zhongzhu 1’ (high-Cd-accumulating population)) with different Cd enrichment characteristics. The two ramie populations were treated with 0, 25, and 75 mg kg⁻¹ Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined. The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ under Cd stress. In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations. Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38–73.10% and 50.05–64.45% Cd accumulating in the ‘Zhongzhu 1’ and ‘Dazhuhuangbaima’ cell wall hemicelluloses, respectively. However, the Cd concentration in the ‘Zhongzhu 1’ hemicellulose was significantly higher (p ≤ 0.05) than that in the ‘Dazhuhuangbaima’ hemicellulose. Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in ‘Zhongzhu 1’ than in ‘Dazhuhuangbaima’ across all Cd treatments. Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall. The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie. It also provides a theoretical basis for Cd enrichment breeding in ramie.

Xyloglucan endotransglycosylase/hydrolase (XTH) genes play an important role in plant resistance to abiotic stress. However, systematic studies of the response of Boehmeria nivea (ramie) XTH genes (BnXTHs) to cadmium (Cd) stress are lacking. We sought to identify the BnXTH-family genes in ramie through bioinformatics analyses and to investigate their responses to Cd stress. We identified 19 members of the BnXTH gene family from the ramie genome, referred to as BnXTH1-19, among which BnXTH18 and BnXTH19 were located on no chromosomes and the remaining genes were unevenly distributed across 11 chromosomes. The 19 members were divided into four groups, Groups I/II/IIIA/IIIB, according to their phylogenetic relationships, and these groups were supported by analyses of intron–exon structure and conserved motif composition. A highly conserved catalytic site (HDEIDFEFLG) was observed in all BnXTH proteins. Additionally, three gene pairs (BnXTH6–BnXTH16, BnXTH8–BnXTH9, and BnXTH17–BnXTH18) were obtained with a fragment and tandem-repeat event analysis of the ramie genome. An analysis of cisregulatory elements revealed that BnXTH expression might be regulated by multiple hormones and abiotic and biotic stress responses. In particular, 17 cisregulatory elements related to abiotic and biotic stress responses and 11 cisregulatory elements related to hormone responses were identified. We also found that most BnXTH genes responded to Cd stress, and BnXTH1, BnXTH3, BnXTH6, and BnXTH15 were most likely to contribute to the Cd tolerance of ramie, as evidenced by the substantial increases in expression under Cd treatment. Heterologous expression of BnXTH1, BnXTH6, and BnXTH15 significantly enhanced the Cd tolerance of transgenic yeast cells. These results suggest that the BnXTH gene family is involved in Cd stress responses, laying a theoretical foundation for functional studies of BnXTH genes and the innovative breeding of Cd-tolerant ramie.

Gibberellin regulates plant growth, development, and metabolic processes. However, the underlying mechanism of the substantial effect of gibberellin on stem height and secondary metabolites in forage ramie is unclear. Therefore, this study combined transcriptomic and metabolomics analyses to identify the mechanisms regulating growth and secondary metabolite contents in forage ramie following exogenous gibberellin application. Exogenous gibberellin application significantly reduced the lignin content in the leaves but not in the stems. At the same time, gibberellin significantly increased the total flavonoid and chlorogenic acid contents in both the stems and leaves. In addition, 293 differentially expressed genes (DEGs) and 68 differentially expressed metabolites (DEMs) were identified in the leaves. In the stems, 128 DEGs and 41 DEMs were identified. The DEGs PER42, FLS, CYP75A, and PNC1 were up-regulated in the leaves, affecting phenylpropane metabolism. The joint analysis of the DEMs and DEGs revealed that the changes in the DEGs and DEMs in the leaves and stems improved the substrate efficiency in the phenol propane pathway and inhibited lignin synthesis in plants, thus shifting to flavonoid pathway synthesis. In conclusion, gibberellin treatment effectively reduces the lignin content in forage ramie while increasing the flavonoid and chlorogenic acid contents. These findings provide empirical and practical guidance for breeding for forage quality in ramie and the improvement and cultivation control of forage ramie.

Ramie (Boehmeria nivea [L.] Gaud.), a nutritious animal feed, is rich in protein and produces a variety of secondary metabolites that increase its palatability and functional composition. Ethylene (ETH) is an important plant hormone that regulates the growth and development of various crops. In this study, we investigated the impact of ETH sprays on the growth and metabolism of forage ramie. We explored the mechanism of ETH regulation on the growth and secondary metabolites of forage ramie using transcriptomic and metabolomic analyses. Spraying ramie with ETH elevated the contents of flavonoids and chlorogenic acid and decreased the lignin content in the leaves and stems. A total of 1076 differentially expressed genes (DEGs) and 51 differentially expressed metabolites (DEMs) were identified in the leaves, and 344 DEGs and 55 DEMs were identified in the stems. The DEGs that affect phenylpropanoid metabolism, including BGLU41, LCT, PER63, PER42, PER12, PER10, POD, BAHD1, SHT, and At4g26220 were significantly upregulated in the leaves. Ethylene sprays downregulated tyrosine and chlorogenic acid (3-O-caffeoylquinic acid) in the leaves, but lignin biosynthesis HCT genes, including ACT, BAHD1, and SHT, were up- and downregulated. These changes in expression may ultimately reduce lignin biosynthesis. In addition, the upregulation of caffeoyl CoA-O-methyltransferase (CCoAOMT) may have increased the abundance of its flavonoids. Ethylene significantly downregulated metabolites, affecting phenylpropanoid metabolism in the stems. The differential 4CL and HCT metabolites were downregulated, namely, phenylalanine and tyrosine. Additionally, ETH upregulated 2-hydroxycinnamic acid and the cinnamyl hydroxyl derivatives (caffeic acid and p-coumaric acid). Cinnamic acid is a crucial intermediate in the shikimic acid pathway, which serves as a precursor for the biosynthesis of flavonoids and lignin. The ETH-decreased gene expression and metabolite alteration reduced the lignin levels in the stem. Moreover, the HCT downregulation may explain the inhibited lignin biosynthesis to promote flavonoid biosynthesis. In conclusion, external ETH application can effectively reduce lignin contents and increase the secondary metabolites of ramie without affecting its growth and development. These results provide candidate genes for improving ramie and offer theoretical and practical guidance for cultivating ramie for forage.