Plant malectin/malectin-like receptor-like kinases (MRLKs) play crucial roles throughout the life course of plants. Here, we identified 23 SiMRLK genes from foxtail millet. All the SiMRLK genes were named according to the chromosomal distribution of the SiMRLKs in the foxtail millet genome and grouped into five subfamilies based on phylogenetic relationships and structural features. Synteny analysis indicated that gene duplication events may take part in the evolution of SiMRLK genes in foxtail millet. The expression profiles of 23 SiMRLK genes under abiotic stresses and hormonal applications were evaluated through qRT-PCR. The expression of SiMRLK1, SiMRLK3, SiMRLK7 and SiMRLK19 were significantly affected by drought, salt and cold stresses. Exogenous ABA, SA, GA and MeJA also obviously changed the transcription levels of SiMRLK1, SiMRLK3, SiMRLK7 and SiMRLK19. These results signified that the transcriptional patterns of SiMRLKs showed diversity and complexity in response to abiotic stresses and hormonal applications in foxtail millet.

In plants, RopGEF-mediated ROP signaling is pivotal in cellular signaling pathways, including apical growth, pollen germination and perception, intercellular recognition, as well as in responses to biotic and abiotic stresses. In this study, we retrieved a total of 37 RopGEF members from three C₄ Crops, of which 11 are from millet, 11 from sorghum, and 15 from maize. Based on their phylogenetic relationships and structural characteristics, all RopGEF members are classified into four subfamilies. The qRT-PCR technique was utilized to evaluate the expression profiles of 11 SiRopGEFs across different tissues in foxtail millet. The findings indicated that the majority of the SiRopGEFs exhibited higher expression levels in leaves as opposed to roots and stems. The levels of expression of SiRopGEF genes were examined in response to abiotic stress and plant hormones. SiRopGEF1, SiRopGEF5, SiRopGEF6, and SiRopGEF8 showed significant induction under abiotic stresses such as salt, cold, and heat. On the other hand, SiRopGEF1, SiRopGEF2, and SiRopGEF7 were consistently upregulated, while SiRopGEF3, SiRopGEF4, SiRopGEF6, SiRopGEF9, and SiRopGEF10 were downregulated upon exposure to abscisic acid (ABA), ethylene (ET), salicylic acid (SA), and gibberellic acid (GA₃) hormones. The alterations in the expression patterns of RopGEF members imply their potential functions in plant growth and development, abiotic stress response, and hormone signal transduction. These discoveries suggest that the RopGEF genes may function as a potential genetic marker to facilitate future studies in elucidating the functional characteristics of RopGEFs.