Carbon sequestration and carbon emission are processes of ecosystem carbon cycling that can be affected while land area converted to grassland resulting in increased soil carbon storage and below-ground respiration. Discerning the importance of carbon cycle in grassland, we aimed to estimate carbon sequestration in photosynthesis and carbon emission in respiration from soil, root, and microbes, for four consecutive years (2007–2010) in a warm-season perennial grassland, Japan. Soil carbon emission increased with increasing growing season temperature which ranged from 438 to 1642 mg CO₂ m⁻² h⁻¹. Four years’ average soil carbon emission for growing season, nongrowing season, and annual emission was 1123, 364, and 1488 g C m⁻², respectively. Nongrowing and snow covered season soil carbon emission contributed 23–25% and 14–17% to the annual emission. Above-ground biomass varied seasonally and variation in green biomass affected soil carbon emission with increasing temperature and precipitation. Temperature effect on root carbon emission contributed about 1/4th of the total soil carbon emission. Variation in soil and root carbon emission is affected by below-ground biomass. Long-term estimation concluded that seasonal and interannual variations in carbon sequestration and emission are very common in grassland ecosystem.

With the increased loss of plant biodiversity, the ex situ seed conservation has become of strategic importance. International treaties and conventions encourage more developed countries to cooperate in providing financial and other supports for the establishment and maintenance of ex situ conservation facilities in developing countries. In this regard, the Nepal Academy of Science and Technology (Nepal) and the Ev-K2-CNR (Italy) set up the Himalayan Seed Bank, with the goal of establishing ex situ seed conservation facility of rare, endemic and endangered plants in Nepal. Here, we discuss the importance of training on seed curation, management and research for the seed bank operators in developing countries. Particular attention is given to the procedures for providing power supply and for optimizing seed longevity in dry storage. Finally, the importance of duplicates in high-standard international seed banks is also discussed.

The predicted increasing atmospheric carbon dioxide (CO2) level is responsive to altering the future climate, and a small change in the soil carbon may significantly affect the forest carbon cycle and climate system. Soil respiration (SR) and its influencing factors like soil temperature (ST), soil water content (SWC) and surface litter-fall were measured monthly over one year in a sub-tropical Pine (Pinus roxburghii) forest of Bhaktapur district located in central Nepal to determine the SR of the forest and, its variations and sensitivity. The results showed that SR varied to the changes in ST by an exponential significant positive correlation between them. The optimum SR was observed between 10 and 22ºC, and the highest SR were obtained above ST at 20ºC. The temperature sensitivity value of SR (Q10) was estimated at Q10 = 2.13. The significant exponential curve represented the effect of SWC on SR. The higher SR rate was mostly measured between 10 and 25% SWC. The monthly and seasonal variations of the SR rate were consistent with the ST, SWC and litter-fall variations. The study showed that the combined effect of temperature and precipitation might be the major cause of SR variations; however, ST is adequate for increasing SR. Hence, the warming further enhances carbon emission from the forest floor and inversely increases carbon to contribute to climatic change through this pine-dominated forest stand structure.

Temperate forests are considered most fragile hence need to recognize their vulnerability owing to continuous climatic changes and anthropogenic activities. In this study, we assessed soil respiration (SR) by using the chamber method in a natural Rhododendron (Rhododendron arboreum) forest which is recognized as the world’s largest forest type located at Annapurna Conservation Area in the temperate region of Nepal. We evaluated the consequences of multiple ecological parameters mainly climatic and biotic factors on SR variations during the month of October in 2016 and 2017. Our results confirmed that SR well corresponded with soil temperature (ST) variables represented with the highly significant ( ) exponential curve (y = 1.049e^0.529x, 2016 and y = 26.34e^0.284x, 2017). And the variation in SR was mediated by a short-range (2-3°C) of ST difference in the month of October during autumn season. However, the effect of soil water content (SWC) on SR was scattered and the photosynthetic photon flux density (PPFD) stood weak to represent the SR variation. The seasonal trend of SR was compatible with the PPFD and litter input with having accountable temporal, diurnal, and interannual variations of SR, ST, SWC, and litterfall. The SR over the entire measurement period were averaged at 269.9 mg CO₂ m⁻²·h⁻¹ in 2016 and 295.1 mg CO₂ m⁻²·h⁻¹ in 2017. Our study manifested that temperate forests could store maximum soil carbon with limited emission through SR and become a larger sink of atmospheric carbon dioxide even though SR is very sensitive to environmental changes and interactively affected by multiple ecological factors. Thus, our finding is an appreciable measure for the temperate forest to understand the regional carbon balance and suggested temperate forests are valued to incorporate them in evaluating global carbon budget.