Aeromonas veronii is a Gram-negative bacterial species that causes disease in fish and is nowadays increasingly recurrent in enteric infections of humans. This study was performed to characterize newly sequenced isolates by comparing them with complete genomes deposited at the NCBI (National Center for Biotechnology Information). Nine isolates from fish, environments, and humans from the São Francisco Valley (Petrolina, Pernambuco, Brazil) were sequenced and compared with complete genomes available in public databases to gain insight into taxonomic assignment and to better understand virulence and resistance profiles of this species within the One Health context. One local genome and four NCBI genomes were misidentified as A. veronii. A total of 239 virulence genes were identified in the local genomes, with most encoding adhesion, motility, and secretion systems. In total, 60 genes involved with resistance to 22 classes of antibiotics were identified in the genomes, including mcr-7 and cphA. The results suggest that the use of methods such as ANI is essential to avoid misclassification of the genomes. The virulence content of A. veronii from local isolates is similar to those complete genomes deposited at the NCBI. Genes encoding colistin resistance are widespread in the species, requiring greater attention for surveillance systems.

Objective: mapping of the distribution of Aedes spp. in the urban area of the municipality of Picos, Piauí associating the climate variables and identification the dengue virus serotypes circulating in Aedes aegypti mosquito populations. Methods: Thirty larvitraps were installed in 10 neighborhoods in the urban area of the municipality. Larvae were collected weekly during the period from February 2017 to July 2019, and the collected material was taken to laboratory for identification and counting. The number of larvae were associated with meteorological data. In adult stage, the A. aegypti mosquitoes were segregated into pools containing six specimens and were then subjected to RT-PCR and Nested-PCR assay for detection of the DENV serotype. Results: a total of 44,798 Aedes spp. larvae were collected in the urban area of Picos during study period. An increase in precipitation and decrease in temperature in the months of February and March contributed to the increase in infestation of these vectors in April. In the study, two larvae of Haemagogus spegazzinii, a potential vector of yellow fever, were collected. The highest occurrence in the municipality was of the species A. aegypti (96.43%) present in all neighborhoods studied with predominance in domiciliary environment, while A. albopictus (3.57%) occur in the peridomiciliary environment. Molecular analyzes of the A. aegypti were positive for DENV 1 and DENV 3 viruses, demonstrating the existence of transovarial transmission. Conclusion: This study showed the simultaneous occurrence of A. aegypti and A. albopictus in the urban area and the co-circulation of dengue serotypes 1 and 3. Entomological and viral circulation monitoring are important tools to indicate areas of greater infestation of vector mosquitoes and prevent future outbreaks, providing results for the actions aimed at the control and prevention of arboviruses, through the secretary of health and competent bodies in the region.

Objective To investigate the epidemiological profile and spatial distribution of human visceral leishmaniasis cases in Crateús, Ceará, Brazil, from 2007 to 2023. Methods This was a retrospective, descriptive cross-sectional study based on epidemiologic data from the Notifiable Health Conditions Information System regarding human visceral leishmaniasis in Crateús between 2007 and 2023. The variables assessed were sex, age group at the time of diagnosis, race/skin color, education level, HIV coinfection, case outcome, neighborhood of residence, area of residence, and number of deaths per year of notification. Relative frequency, incidence, and lethality rates were calculated; maps were created to reflect the spatial distribution of cases within the municipality. Results Between 2007 and 2023, 112 cases of visceral leishmaniasis were reported in Crateús, predominantly among males (n=77), brown-skinned individuals (n=81), aged 20-39 years (n=27), with incomplete elementary education (n=31), and residing in urban areas of the municipality (n=88). The incidence rate decreased from 22.81 in 2007 to 16.02 in 2008, with a peak of 14.67 in 2018; by 2023, the incidence rate had dropped to 10.47. There were four cases of HIV coinfection and two deaths directly attributable to the disease. Conclusion Cases of visceral leishmaniasis in Crateús were concentrated in the urban area of the municipality. The epidemiologic profile was consistent with national trends, with the most affected individuals being of mixed race/skin color, male, aged 20-39 years, and with incomplete elementary education.

Resumo Objetivo Investigar o perfil epidemiológico e distribuição espacial dos casos de leishmaniose visceral humana em Crateús no período 2007-2023. Métodos Tratou-se de estudo transversal, retrospectivo descritivo, baseado em dados epidemiológicos oriundos do Sistema de Informação de Agravos de Notificação referentes à leishmaniose visceral humana em Crateús de 2007 a 2023. Foram avaliados sexo, faixa etária do diagnóstico, raça/cor da pele, escolaridade, coinfecção com HIV, evolução do caso, bairro de residência, zona de residência e número de óbitos por ano de notificação. Foram calculadas a frequência relativa e as taxas de incidência e letalidade; mapas foram confeccionados para refletir a distribuição dos casos da doença no município. Resultados Entre 2007 e 2023, ocorreram 112 casos de leishmaniose visceral em Crateús, predominantemente em indivíduos do sexo masculino (n=77), pardos (n=81), na faixa etária 20-39 anos (n=27), com ensino fundamental incompleto (n=31) e residentes na zona urbana do município (n=88). Houve redução da taxa de incidência de 22,81 em 2007 para 16,02 em 2008, destacando-se o aumento para 14,67 em 2018; em 2023, a taxa de incidência foi 10,47. Houve coinfecção com o HIV em quatro casos. Foram registrados dois óbitos em decorrência direta da doença. Conclusão. Os casos de leishmaniose visceral em Crateús concentraram-se na zona urbana do município. O perfil epidemiológico mostrou-se alinhado à tendência nacional, sendo indivíduos pardos, sexo masculino, faixa etária 20-39 anos e com ensino fundamental incompleto os mais afetados pela doença.