Sea buckthorn ( Hippophae rhamnoides L.), a wild berry abundant in Georgia, is a rich source of bioactive compounds, including antioxidants, fatty acids, and phenolic compounds, with significant potential for applications in food, nutraceutical, and cosmetic industries. Despite its traditional uses, the physicochemical properties and valorization potential of Georgian sea buckthorn remain underexplored. This study aimed to analyze the chemical composition of sea buckthorn berries from three distinct regions of Georgia (Adjara, Samtskhe‐Javakheti, and Imereti) and develop innovative, environmentally friendly extraction technologies to maximize the recovery of valuable compounds. Using advanced techniques such as ultrasound‐assisted extraction (UAE) and supercritical water extraction (SWE), alongside traditional methods like maceration and Soxhlet extraction, the study identified and quantified 18 bioactive compounds, including organic acids (malic and citric acid), phenolic acids (quinic acid), and flavonoids (e.g., gallocatechin, catechin, and isorhamnetin derivatives). The lipid fraction, predominantly composed of C18 fatty acids (57.4%–76.9%), was rich in linoleic acid (29.4%–50.0%), with extraction yields varying by method. Total phenolic content ranged from 19.90 to 34.94 mg/g dry weight, with higher levels in Samtskhe‐Javakheti and Imereti samples. The highest carotenoid content (3.363 mg/g dry weight) was observed in Samtskhe‐Javakheti berries. UAE with 75% ethanol optimized carotenoid (1.684 mg/g) and phenolic (8.213 mg/g) recovery, while SWE achieved 86% lipid yield and high phenolic content (92.25 mg/g) in just 7 min. Carotenoid‐enriched sunflower oil exhibited 3.5‐fold higher antioxidant activity than control oil, demonstrating enhanced oxidative stability. The study highlights the efficacy of green extraction methods in valorizing sea buckthorn by‐products, offering sustainable solutions for producing high‐value nutraceuticals and functional foods. These findings underscore the potential of Georgian sea buckthorn as a source of natural antioxidants and lipids, with applications in health‐promoting products.

The study determined the content of lycopene in the fruits of the Elaeagnus umbellata (35.25–60.21 mg/100 g), common at different heights above sea level in Western Georgia. For the effective extraction of lycopene as a biologically active substance, the optimal conditions for ultrasonic extraction were selected: sunflower oil was used as a “green solvent”; the ratio of solid mass and solvent was 1:50; temperature 30°C; ultrasound amplitude 40%; power 85 W; and extraction time 10 min. FTIR spectra revealed the characteristic functional groups of lycopene exhibiting two characteristic peaks at 2920 and 2950 cm⁻¹. To explore the effect of lycopene on oil quality, the acid value, peroxide value, and p‐anisidine were determined in each oil sample. The antioxidant determination by inhibition of DPPH radicals showed significant differences in native oils and oils with lycopene.

Hippophae rhamnoides L. is a valuable medicinal plant, distinguished by its rich content of bioactive compounds, mainly carotenoids and phenols. This study aimed to optimize the extraction of carotenoids from H. rhamnoides L. fruits using "green technologies" (ultrasonic bath and probe), as well as to evaluate the effect of various drying methods (atmospheric, vacuum, and freeze-drying) on carotenoid content and antioxidant activity. The research object was H. rhamnoides L. fruits, wild-growing in Western Georgia. Quantitative determination of carotenoids was carried out by the spectrophotometric method, and antioxidant activity was assessed by the DPPH radical scavenging method. The results of the study showed that ultrasonic probe extraction provides a high yield of carotenoids (1.564–3.363 mg/g dry weight), which significantly exceeds the values obtained by the classical method (1.111–2.944 mg/g). A correlation was established between carotenoid concentration and antioxidant activity. In particular, a high content of carotenoids (Akhaltsikhe, 3.363 mg/g) provides 50% inhibition of the DPPH radical with a relatively small amount of the sample (2.881 mg). Among the drying methods, freeze-drying (2.689 mg/g) and vacuum drying (2.481 mg/g) showed an advantage in preserving carotenoid content and antioxidant activity compared to atmospheric drying (1.778 mg/g). In conclusion, the use of "green technologies" is effective for obtaining bioactive compounds, which contribute to their use in ophthalmology, especially for the regeneration of eye surface damage. The data obtained forms the basis for further in vitro and in vivo studies.