Mediterranean agriculture is increasingly constrained by severe and recurrent water scarcity. Rising temperatures, declining precipitation, and more frequent droughts are driving higher crop water demand and reducing rainfall reliability. Combined with growing competition for limited freshwater resources, these climatic pressures pose a major challenge to the long-term sustainability and productivity of farming systems in the region. In this context, urban treated wastewater (UTWW) is increasingly recognized as a strategic alternative resource. Regulation (EU) 2020/741 establishes minimum quality requirements for agricultural water reuse based on the fit-for-purpose principle. The Italian regulatory framework requires regional authorities to plan water reuse by mapping reclamation facilities, water demand, and distribution networks. From this perspective, to support the planning process, this study describes the application of a Geographic Information System (GIS)-based methodology to assess the potential for UTWW reuse in Sicily by integrating national datasets (regularly updated) and comparing reclaimed water supply with irrigation demand from both qualitative and quantitative perspectives. UTWW supply was estimated by integrating data from the Italian National Institute of Statistics (ISTAT) Census of Water for Civil Use and the European Environment Agency (EEA) dataset, while irrigation demand was derived by combining Copernicus crop data, ISTAT irrigated-to-total UAA ratios and crop-specific irrigation demand (Seasonal Specific Volumes) derived from the SIGRIAN database. Two scenarios were analyzed for grasslands, vineyards, olive groves, and orchards. Scenario 1 included only wastewater treatment plants (WWTPs) located inside irrigation areas, whereas Scenario 2 also included selected WWTPs located outside irrigation areas where orographic and distance constraints allowed potentially feasible connections. Under Regulation (EU) 2020/741, potential compatibility with the required water quality classes was identified at the screening level for all crop categories considered, which require classes B, C or D. From a quantitative perspective, Scenario 1 identified 19 WWTPs serving 11 out of 38 irrigation areas with an aggregated uncapped demand coverage of 53%; only two areas reached full local demand satisfaction. Scenario 2 added 23 external WWTPs, increased potentially served areas to 20, and raised the aggregate uncapped coverage to 73%. When surplus volumes were capped within each irrigation area, effective demand satisfaction was 12% and 22% in Scenarios 1 and 2, respectively, highlighting the importance of storage, conveyance and inter-area transfer. The results indicate that treated wastewater reuse could contribute to agricultural water security and climate-resilient water management in Mediterranean regions. The framework supports the first-order identification of candidate reuse areas, but site-specific assessments of effluent quality, risk management, costs, and seasonal storage remain necessary before implementation.