Cancer is a serious health problem due to the complexity of establishing an effective treatment. The purpose of this work was to evaluate the activity of a triazaspirane as a migration and invasion inhibitor in PC3 prostatic tumor cells through a possible negative regulation of the FAK/Src signal transduction pathway and decreased secretion of metalloproteinases 2 and 9. Molecular docking analysis was performed using Moe 2008.10 software. Migration (wound-healing assay) and invasion (Boyden chamber assay) assays were performed. In addition, the Western blot technique was used to quantify protein expression, and the zymography technique was used to observe the secretion of metalloproteinases. Molecular docking showed interactions in regions of interest of the FAK and Src proteins. Moreover, the biological activity assays demonstrated an inhibitory effect on cell migration and invasion, an important suppression of metalloproteinase secretion, and a decrease in the expression of p-FAK and p-Src proteins in treated PC3 cells. Triazaspirane-type molecules have important inhibitory effects on the mechanisms associated with metastasis in PC3 tumor cells.

Cancer is known as a microevolutionary disease associated with genetic and epigenetic modifications, which is considered a global problem for which there is no fully effective treatment. Prostate cancer (PCa) is the second most commonly diagnosed cancer in men, with 1,414,259 new global cases (14.1%) in 2020. The aim of the present study was to evaluate the activity of a molecule of the triazaspirane family in PC3 PCa cells, through possible negative regulation of the FAK/Src signal transduction pathway. A molecular docking assay was performed using the compound 1,3,8-triazaspiro[4.5]decane-2,4-dione (TSDD) against FAK and Src. Migration and invasion assays were performed on PC3 PCa cells. In addition, the protein expression levels of phosphorylated p-FAK, p-Src, E-cadherin and N-cadherin were determined, and MMP-9 secretion was analysed. Molec-ular docking assays showed interactions in the regions of interest of FAK and Src proteins, and biological activity assays demonstrated an inhibitory effect on cell migration and in-vasion. In addition, a significant suppression of MMP-9 secretion was detected in treated PC3 cells, as well as a decrease in the expression of p-FAK and p-Src proteins. In conclu-sion, the TSDD molecule demonstrated inhibitory effects related to cell migration and in-vasion in an in vitro model of PCa.