Sediment, a special realm in aquatic environments, has high microbial diversity. While there are numerous reports about the microbial community in marine sediment, freshwater and intertidal sediment communities have been overlooked. The present study determined millions of Illumina reads for a comparison of bacterial communities in freshwater, intertidal wetland, and marine sediments along Pearl River, China, using a technically consistent approach. Our results show that both taxon richness and evenness were the highest in freshwater sediment, medium in intertidal sediment, and lowest in marine sediment. The high number of sequences allowed the determination of a wide variety of bacterial lineages in all sediments for reliable statistical analyses. Principal component analysis showed that the three types of communities could be well separated from phylum to operational taxonomy unit (OTU) levels, and the OTUs from abundant to rare showed satisfactory resolutions. Statistical analysis (LEfSe) demonstrated that the freshwater sediment was enriched with Acidobacteria , Nitrospira , Verrucomicrobia , Alphaproteobacteria , and Betaproteobacteria . The intertidal sediment had a unique community with diverse primary producers (such as Chloroflexi , Bacillariophyta , Gammaproteobacteria , and Epsilonproteobacteria ) as well as saprophytic microbes (such as Actinomycetales , Bacteroidetes , and Firmicutes ). The marine sediment had a higher abundance of Gammaproteobacteria and Deltaproteobacteria , which were mainly involved in sulfate reduction in anaerobic conditions. These results are helpful for a systematic understanding of bacterial communities in natural sediment environments.