Accurate detection and timely treatment of component defects in substations is an important measure to ensure the safe operation of power systems. In this study, taking substation meters as an example, a dataset of common meter defects, such as a fuzzy or damaged dial on the meter and broken meter housing, is constructed from the images of manual inspection in power systems. There are several challenges involved in accurately detecting defects in substation meter images, such as the complex background, different meter sizes and large differences in the shapes of meter defects. Therefore, this paper proposes the PHAM-YOLO (Parallel Hybrid Attention Mechanism You Only Look Once) network for automatic detection of substation meter defects. In order to make the network pay attention to the key areas against the complex background of the meter defect images and the differences between different defect features, a Parallel Hybrid Attention Mechanism (PHAM) module is designed and added to the backbone of YOLOv5. PHAM integration of local and non-local correlation information can highlight these differences while remaining focused on the meter defect features. To improve the expressive ability of the feature map, a Spatial Pyramid Pooling Fast (SPPF) module is introduced, which pools the input feature map using a continuous fixed convolution kernel, fusing the feature maps of different receptive fields. Bounding box regression (BBR) is the key way to determine object positioning performance in defect detection. EIOU (Efficient Intersection over Union) is, therefore, introduced as a boundary loss function to solve the ambiguity of the CIOU (Complete Intersection Over Union) loss function, making the BBR regression more accurate. The experimental results show that the Average Precision Mean (mAP), Precision (P) and Recall (R) of the proposed PHAM-YOLO network in the dataset are 78.3%, 78.3%, and 79.9%, respectively, with mAP being improved by 2.7% compared to the original model and higher than SSD, Fast R-CNN, etc.

Acinetobacter baumannii (A. baumannii) is among the main pathogens that cause nosocomial infections. The ability to rapidly and accurately detect A. baumannii and its drug resistance is essential for blocking secondary infections and guiding treatments. In this study, we reported a nucleic acid fluorescent lateral flow assay (NFLFA) to identify A. baumannii and carbapenem-resistant A. baumannii (CRAB) in a rapid and quantitative manner by integrating loop-mediated isothermal amplification (LAMP) and silica–based multilayered quantum dot nanobead tag (Si@MQB). First, a rapid LAMP system was established and optimised to support the effective amplification of two bacterial genes in 35 min. Then, the antibody-modified Si@MQB was introduced to capture the two kinds of amplified DNA sequences and simultaneously detect them on two test lines of a LFA strip, which greatly improved the detection sensitivity and stability of the commonly used AuNP-based nucleic acid LFA. With these strategies, the established LAMP-NFLFA achieved detection limits of 199 CFU/mL and 287 CFU/mL for the RecA (house-keeping gene) and bla_OXA-23 (drug resistance gene) genes, respectively, within 43 min. Furthermore, the assay exhibited good repeatability and specificity for detecting target pathogens in real complex specimens and environments; thus, the proposed assay undoubtedly provides a promising and low-cost tool for the on-site monitoring of nosocomial infections.

Detecting cracks from images plays a crucial role in road maintenance. Road cracks exhibit significant diversity and complexity in terms of shape, size, texture, and road images may contain various noises and interferences such as lighting variations, shadows, and different appearances due to varying perspectives and scales. To address these challenges, we constructed a comprehensive dataset called the Comprehensive Road Crack Dataset (CRCrack Dataset), which encompasses various crack characteristics. In this study, we propose a road crack segmentation network called CSegNet (Crack Segmentation Network), which combines convolutional neural networks (CNNs) and Transformers. The network adopts an encoder-decoder framework, like DeepLab V3+. In the encoder, leveraging the flexibility of Transformers in modeling long-term dependencies and the ability of CNNs to capture local contextual information through local receptive fields, weight sharing, and spatial subsampling, we design a ResNeXTR (ResNeXt-Transformer) feature extraction module as the backbone network to enhance the feature extraction capability for road crack images. To reduce the computational cost in self-attention computation of transformer, we introduce an average pooling layer to downsample the dimensions of the encoded features. In the decoder, to focus on the key information of road cracks under diverse environmental conditions and interferences, we combine the Efficient Channel Attention Module (ECAM) and the Spatial Attention Module (SAM) to design an Efficient Convolutional Block Attention Module (ECBAM) attention module to further optimize feature representation. Additionally, we employ the ReLU activation function, SGD gradient descent, and a hybrid loss function of Binary Cross Entropy with Logits to accelerate convergence speed and improve segmentation accuracy. Through comparative experiments on the CRCrack dataset, the results demonstrate that our proposed method outperforms classic networks such as U-Net and DeepLab V3 + in terms of IoU, Dice, and AUROC evaluation metrics. It exhibits good adaptability to ground crack images from different sources, providing a basis for estimating the degree of road damage.