A Gram-stain-negative, aerobic, non-motile, short-rod-shaped bacterium, designated as strain TC11T, was isolated from rhizosphere soil of mangrove forest (Kandeliaobovata) in Fugong village, Zhangzhou, Fujian, China. Strain TC11T grew at 15–45 °C (optimum, 35 °C), 0–8 % (w/v) NaCl (optimum, 1 %, w/v) and pH 5.5–9.5 (optimum, pH 7.5). Phylogenetic analyses revealed that strain TC11T belonged to a clade of the genus Pseudomonas and showed the highest sequence similarity of 98.4 % to Pseudomonas fluvialis ASS-1T, followed by Pseudomonas oleovorans subsp. oleovorans DSM 1045T (97.9 %), Pseudomonas indoloxydans JCM 14246T (97.7 %), Pseudomonas guguanensis JCM 18416T(97.6 %) and Pseudomonas alcaliphila JCM 10630T (97.5 %) on the basis of their 16S rRNA gene sequences. The DNA G+C content was 64.3 mol%. In silico DNA–DNA hybridization and average nucleotide identity values between strain TC11T and the reference strains were 19–22 % and 72–78 %, respectively. Studies based on the three housekeeping genes, rpoB, gyrB and rpoD, further confirmed that strain TC11T is a novel member of the genus Pseudomonas . The major fatty acids of strain TC11Twere C16 : 0, summed feature 8 (C18 : 1ω6c/C18 : 1ω7c) and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The sole isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain TC11T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas mangrovi sp. nov., is proposed. The type strain is TC11T (=KCTC 62159=MCCC 1K03499).

A novel Gram-stain-negative, rod-shaped, non-motile strain, designated GKXT, was isolated from deep seawater. Strain GKXT was able to grow at 20–35 °C (optimum, 25 °C), pH 5.5–9.5 (optimum, 7.5) and 0–4.0 % (w/v) NaCl (optimum, 1.0 %). The major fatty acids were C16 : 1ω9c (15.4 %), C16 : 0 (18.4 %), C14 : 0 (12.0 %), iso-C14 : 0 (30.1 %) and anteiso-C15 : 0 (5.7 %). Strain GKXT contained phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified glycolipid as the main polar lipids. The only isoprenoid quinone was menaquinone-9. The diagnostic amino acids of the cell-wall peptidoglycan contained meso-diaminopimelic acid. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GKXT belonged to the genus Luteolibacter in the family Verrucomicrobiaceae . The 16S rRNA gene sequence of this strain showed 98.0, 93.5 and 93.3 % sequence similarity, respectively, with those of Luteolibacter arcticus MC 3726T, L.uteolibacter pohnpeiensisA4T-83T and L.uteolibacter cuticulihirudinis E100T. DNA–DNA hybridization value of strain GKXT with L. arcticus MC 3726T was 33.1 %. The G+C content of the genomic DNA was 59.5 mol%. On the basis of the genotypic, phenotypic, phylogenetic and chemotaxonomic characteristics, strain GKXT was proposed to represent a novel species of the genus Luteolibacter , named Luteolibacter flavescens sp. nov. (type strain GKXT=MCCC 1K03193T=KCTC 52361T).

An anaerobic bacterium, strain ZC-CMC3T, was isolated from a wastewater sample in Zhejiang, China. Cells were Gram-stain-positive, peritrichous, non-spore-forming, rod-shaped (0.6–1.2 × 2.9–5.1 μm) and catalase- and oxidase-negative. Strain ZC-CMC3T was able to grow at 25–48 °C (optimum 43 °C) and pH 5.5–8.0 (optimum pH 7.0). The NaCl concentration range for growth was 0–3 % (w/v) (optimum 0 %). The major polar lipids of the isolate were diphosphatidylglycerol, phosphatidylglycerol, several phospholipids and glycolipids. Main fermentation products from PYG medium were formate, acetate, lactate and ethanol. Substrates which could be utilized were peptone, tryptone, yeast extract and beef extract. No respiratory quinone was detected. The main fatty acids were C14 : 0, C16 : 0, C16 : 1 cis 7 and C16 : 1 cis 9. The DNA G+C content was 30.0 mol%. 16S rRNA gene sequence analysis revealed that the isolate belonged to the family Clostridiaceae. Phylogenetically, the most closely related species were Oceanirhabdus sediminicola NH-JN4T (92.8 % 16S rRNA gene sequence similarity) and Clostridium tepidiprofundi SG 508T (92.6 %). On the basis of phylogenetic, chemotaxonomic and phenotypic characteristics, strain ZC-CMC3T represents a novel species of a new genus in the family Clostridiaceae, for which the name Haloimpatiens lingqiaonensis gen. nov., sp. nov. is proposed. The type strain of the type species is ZC-CMC3T ( = KCTC 15321T = JCM 19210T = CCTCC AB 2013104T).