Leaf area (LA) and SPAD value (a proxy for chlorophyll content) are two key determinants of seedling quality. This study aimed to develop and validate approaches for the efficient retrieval of these features in order to facilitate both management and screening practices. In cucumber, different models were developed and tested for the accurate estimation of LA at the scale of the individual organ (cotyledon, leaf) by using its linear dimensions (length (L) and width (W)), and of the whole seedling by using the 2D image-extracted projected area (from three different angles: 0°, 45°, and 90°). At either scale, the SPAD value was computed by using image (90°)-based colorimetric features. The estimation of individual organ area was more accurate when using L alone, compared with W alone. By using the two dimensions and specific colorimetric features, the individual organ area (R2 ≥ 0.92) and SPAD value (R2 of 0.77) were accurately predicted. When considering a single view, the top one (90°) was associated with the highest accuracy in whole-seedling LA estimation, and the side view (0°) with the lowest (R2 of 0.88 and 0.73, respectively). Using any combination of two angles, the whole-seedling LA was accurately retrieved (R2 ≥ 0.88). When using colorimetric features, a poor estimation of the whole-seedling SPAD value was noted (R2 ≤ 0.43). The deployment of artificial neural networks (ANNs) further allowed the estimation of specific organ shape traits, and improved the accuracy of all the aforementioned predictions, including the whole-seedling SPAD value (R2 of 0.597). In conclusion, the findings of this study highlight that features readily retrieved from 2D images hold promising potential for improving screening routines within the nursery industry.
Water loss during storage is a major cause of postharvest quality deterioration in cucumber, yet existing methods to monitor hydration are often destructive or require expensive instrumentation. We developed a low-cost, non-destructive approach for estimating fruit relative water content (RWC) using visible-light color imaging combined with an ensemble machine-learning model (Random Forest). A total of 1200 fruits were greenhouse-grown, harvested at market maturity, and equally divided between optimal and ambient storage temperature (10 and 25 °C, respectively). Digital images were acquired at harvest and at 7 d intervals during storage, and color parameters from four standard color systems (RGB, CMYK, CIELAB, HSV) were extracted separately for the neck, mid, and blossom regions as well as for the whole fruit. During storage, fruit RWC decreased from 100% (fully hydrated condition) to 15.3%, providing a broad dynamic range for assessing color–hydration relationships. Among the 16 color features evaluated, the mean cyan component (μC) of the CMYK space showed the strongest relationship with measured RWC (R2 up to 0.70 for whole-fruit averages), reflecting the cyan region’s heightened sensitivity to dehydration-induced changes in pigments, cuticle properties and surface scattering. The Random Forest regression model trained on these features achieved a higher predictive accuracy (R2 = 0.89). Predictive accuracy was also consistently higher when μC was calculated over the entire fruit surface rather than for individual anatomical regions, indicating that whole-fruit color information provides a more robust hydration signal than region-specific measurements. Our findings demonstrate that simple visible-range imaging coupled with ensemble learning can provide a cost-effective, non-invasive tool for monitoring postharvest hydration of cucumber fruit, with direct applications in quality control, shelf-life prediction and waste reduction across the fresh-produce supply chain.
Automated greenhouse production systems frequently employ non-destructive techniques, such as computer vision-based methods, to accurately measure plant physiological properties and monitor crop growth. By utilizing an automated image acquisition and analysis system, it becomes possible to swiftly assess the growth and health of plants throughout their entire lifecycle. This valuable information can be utilized by growers, farmers, and crop researchers who are interested in self-cultivation procedures. At the same time, such a system can alleviate the burden of daily plant photography for human photographers and crop researchers, while facilitating automated plant image acquisition for crop status monitoring. Given these considerations, the aim of this study was to develop an experimental, low-cost, 1-DOF linear robotic camera system specifically designed for automated plant photography. As an initial evaluation of the proposed system, which targets future research endeavors of simplifying the process of plant growth monitoring in a small greenhouse, the experimental setup and precise plant identification and localization are demonstrated in this work through an application on lettuce plants, imaged mostly under laboratory conditions.
1 July 2014 in The International Journal of Prosthodontics
This study aimed to develop a classification of edentulous jaws for use as a diagnostic tool during implant-prosthodontic treatment planning. The morphology of 200 fully edentulous alveolar ridges (100 maxillae, 100 mandibles) was assessed with cone beam computed tomography. Generic implants (length: 8 mm; diameter: 4.1 mm) were used. To develop the classification system, the feasibility of virtually placing the implants without vertical ridge augmentation was considered. Potential implant sites were evaluated in terms of ridge width and described as either type A (no horizontal augmentation required) or type B (horizontal augmentation required). A descriptive statistical analysis of subjects' age, sex, and arch classification was performed. In total, 880 implants were virtually planned. Based on alveolar ridge height, four arch patterns were identified (C1 to C4), providing a basis for prosthodontic planning with either removable or fixed implant-supported restorations. The frequencies of each category were as follows: C3 (n = 62, 62%), C4 (n = 16, 16%), C2 (n = 12, 12%), and C1 (n = 10, 10%) for the maxilla and C3 (n = 36, 36%), C4 (n = 31, 31%), C1 (n = 24, 24%), and C2 (n = 9, 9%) for the mandible. The proposed classification of the edentulous arch represents a useful tool for communication between clinicians when planning implant-supported rehabilitations.