Theanine content is highly correlated with sensory quality and health benefits of tea infusion. The tender shoots of etiolated and albino tea plants contain higher theanine than the normal green tea plants and are valuable materials for high quality green tea processing. However, why these etiolated or albino tea plants can highly accumulate theanine is largely unknown. In this study, we observed an Arabidopsis etiolated mutant hy1-100 (mutation in Haem Oxygenase 1, HO1) accumulated higher levels of glutamine (an analog of theanine). We therefore identified CsHO1 in tea plants, and found CsHO1 is conserved in amino acid sequences and subcellular localization with its homologs in other plants. Importantly, CsHO1 expression in the new shoots was much lower in an etiolated tea plants “Huangkui” and an albino tea plant “Huangshan Baicha” than that in normal green tea plants. The expression levels of CsHO1 were negatively correlated with theanine contents in these green, etiolated and albino shoots. Moreover, CsHO1 expression levels in various organs and different time points were also negatively correlated with theanine accumulation. The hy1-100 was hypersensitive to high level of theanine and accumulated more theanine under theanine feeding, and these phenotypes were rescued by the expression of CsHO1 in this mutant. Transient knock-down CsHO1 expression in the new shoots of tea plant using antisense oligonucleotides (asODN) increased theanine accumulation. Collectively, these results demonstrated CsHO1 negatively regulates theanine accumulation in tea plants, and that low expression CsHO1 likely contributes to the theanine accumulation in etiolated/albino tea plants.

High labor costs and labor shortages are limiting factors affecting the tea industry in Anhui Province. Thus, exploiting the full mechanization of shoot harvesting is an urgent task in the tea industry. Tea quality is greatly influenced by the integrity rate of tea leaves; therefore, it is important to choose tea cultivars suitable for machine picking. In this study, seven tea cultivars were used to investigate the relationship between internode length and blade angle with respect to newly formed tea shoots and machine harvesting in field experiments (Xuanchen City, Kuiling village) conducted throughout the year (in the autumn of 2021, in the early spring of 2022, and in the summer of 2022). Our results showed that the internode length (L2 or L4) had a significant and positive correlation with the integrity rate of tea buds and leaves in seven tea cultivars over three seasons. However, no significant correlation was found between the blade angle and the integrity rate of tea buds and leaves. In addition, a strong and positive correlation was found between the levels of GA₁ (R2 > 0.7), GA₃ (R2 > 0.85), and IAA (R2 > 0.6) regarding the internodes and internode lengths of the seven tea cultivars. Moreover, the relative expression levels of CsGA20ox, CsGA3ox1, and CsGA3ox2 in Echa1 (the longer internode) were significantly higher compared with those in Zhenong113 (the shorter internode). Overall, our results show that the internode length is an important factor for the machine harvesting of tea leaves and that the level of GA3 is strongly associated with internode length.

Light, as a critical environmental factor, plays a pivotal role in photosynthesis, ultimately influencing the timing of bud flush in tea plants. However, the synergistic effects of different photoperiods and light qualities on the timing of bud flush in the albino tea cultivar ‘HuangKui’ (later germination variety) remain unknown. Thus, the objective of this study was to investigate the effects of different photoperiods (12L/12D, 14L/10D, 16L/8D, and 18L/6D, where L = the number of daylight hours and D = the number of hours of darkness) and ratios of red (R) to blue (B) light (R/B 1:1, R/B 1:2, R/B 1:3, and R/B 2:1) on the germination and growth of the albino tea variety ‘HuangKui’. In our study, we examined how different photoperiods and red light and blue light affected tea germination and growth by investigating the timing of bud flush, photosynthesis, chlorophyll content, and growth indicators. First, our study showed that ‘HuangKui’ germinated 4 days, 2 days, and 1 day earlier under the 16L/8D photoperiod at the one bud and one leaf period compared with plants cultivated under the 12L/12D, 14L/10D, and 18L/6D photoperiods under light simulating the solar spectrum. Also, the growth of ‘HuangKui’ was maximumly promoted under the 16L/8D photoperiod treatment. Additionally, the earliest germination of ‘HuangKui’ was observed for the 16L/8D photoperiod under the R/B 2:1 (red/blue) treatment compared with the other treatments. Moreover, the greatest plant height, length of the new shoots, and new leaf areas were detected in the albino tea variety ‘HuangKui’ under R/B 2:1. Moreover, the contents of auxin (indole acetic acid, IAA) and trans-zeatin (tZ) under R/B 2:1 were significantly higher than those under the R/B 1:1 and control treatments with the 16L/8D photoperiod. Additionally, the auxin-related expression levels of CsIAA13, CsGH3.1, CsAUX1, and CsARF2 under the R/B 2:1 treatment were significantly higher than those in the control. The expression of CsARR-B, a positive regulator of cytokinin-related genes, was significantly higher under the R/B 2:1 treatment than under the control treatment, while the opposite result was found for the expression of the negative regulator CsARR-A. Therefore, the R/B 2:1 treatment with the 16L/8D photoperiod was an appropriate means of timing the bud flush for the albino tea variety ‘HuangKui’, which may be related to IAA or tZ signal transduction. In conclusion, our research offers a novel lighting strategy that promotes the germination and growth of albino tea cultivars.

Sandy loam soils are widespread and important for agricultural soil use. The lateral stress caused by the loosening shovel of a subsoiler produces lateral disturbances in sandy loam soil, reducing compaction and improving soil structure. To explore the variation and transmission of lateral stress due to the operation of a double-wing subsoiler in sandy loam soil, a lattice of point-type soil sensors was arranged in a soil bin, and the lateral stress generated by subsoiling was measured in the shallow, medium, and deep soil layers. The experimental results show that when the lateral stress generated by the double-wing subsoiler is transferred to the lateral position of different distances.The lateral stress shows a sinusoidal fluctuation law with time, the sine wave frequency range is: shallow (0.366–0.549); middle (0.306–0.494); and deep (0.088–0.501). There were greater fluctuation amplitudes in the transfer process for the shallow and deep soil layers than for the middle layer. Shallow soil had the largest final stable lateral stress (0.1617 N), and middle soil the smallest (0.0733 N). Thus, lateral stress from deep loosening has the greatest effect on shallow soil and the least effect on middle soil. These results indicate that the fluctuating lateral stresses generated by double-wing-subsoiler operation drive a fluctuating soil disturbance that can improve soil structure, and that they are greater for shallow and deep soils than for middle soils.

Sandy clay loam has the characteristics of both sand and clay. Because of these characteristics, both frictional resistance and adhesive resistance occur between the soil and tillage tool. The combined effect of the two frictional forces increases the external friction angle between the soil and tillage tool, thus increasing the working resistance. To address this issue, this study investigated the coupling effect of high pressure and hot air on the external friction angle by using a self-developed device to measure the external friction angle. Test results showed that high-pressure air between the soil and tillage tool formed a high-pressure air curtain layer which acted as a lubricant, thereby reducing the external friction angle. The external friction angle decreased as the airflow pressure increased. The reduction in the moisture content of the subsoil to less than 30% by the high-pressure hot air reduced the resistance between the soil and tillage tool. The approach with the high-pressure hot air curtain was verified in tests on a subsoiling shovel; the working resistance of the shovel under high-pressure hot air was reduced by 14.8%, demonstrating that this approach was effective in reducing the working resistance of the shovel.