LukS-PV is a component of Panton-Valentine leucocidin (PVL) and is secreted by Staphylococcus aureus. Silver nanoparticles exhibit considerable potential as anticancer agents and drug delivery systems. Drug delivery is a way to deliver medicinal combinations to achieve a beneficial therapeutic effect. In the current study, recombinant LukS-PV protein-loaded silver nanoparticles were prepared and their cytotoxicity effect was analyzed on human breast cancer cells and human normal embryonic kidneys cells by MTT assay. Apoptosis was investigated by staining with Annexin V/propidium iodide. The recombinant LukS-PV protein-loaded silver nanoparticles showed dose‐dependent cytotoxicity and induced apoptosis in the MCF7 cells and had a lesser effect on HEK293 cells. After 24 h exposure to the recombinant LukS-PV protein-loaded silver nanoparticles (IC50), Annexin V-FITC/PI FCM revealed that 33.2% of MCF7 cells were apoptotic. In conclusion, recombinant LukS-PV protein-loaded silver nanoparticles probably cannot be a better alternative for the targeted healing approaches to cancer therapies. Hence, it is suggested that silver nanoparticles could be utilized as a delivery system for releasing toxins into cancer cells.

Background and objectives: Drug resistance in bacteria is one of the important problems in the antibacterial field. Therefore, new drugs and therapeutic approaches are required to eliminate bacteria using different and novel mechanisms. Among these, the silver nanoparticles have been proposed as a substance with antibacterial properties against gram-positive and gram-negative bacteria. The present study aimed to investigate the effects of silver nanoparticles with a size of less than 20 nm on the genome of Staphylococcus aureus (S. aureus) as a model for gram-positive bacteria.Material and methods: For this purpose, the bacteria were treated at concentrations of 100 and 150 µg/ml nanoparticles and antimicrobial properties of the nanoparticles were investigated in intervals of 2, 4 and 24 hours, then DNA was extracted. RAPD molecular marker was used to investigate the effects of nanoparticles on the genome. In addition, the results of electrophoresis for polymerase chain reaction (PCR) products on agarose gel were analyzed.Results: The present findings demonstrated that silver nanoparticles not only have an inhibitory effect on bacteria but also affect the genomic DNA sequence of this bacterium and change it in different sites.Conclusion: The nanoparticles are antibacterial compounds and can be an appropriate alternative to antibiotics.

Background & Aims: Breast cancer is the most common type of malignancy among women. Today, various methods of chemotherapy, surgery, and radiation therapy are used to treat cancer. However, among the disadvantages and side effects of these methods are the destruction of normal cells. This has led researchers to turn ...