Objective: To analyze the glutathione antioxidant defense system changes in the tear and serum of patients with hypertensive retinopathy (HR) and to establish whether there is an interdependence between their levels and HR degree.

Background. Hypertension (HTN) is one of the leading causes of morbidity and mortality worldwide. A prompt diagnosis and treatment of hypertensive retinopathy (HR), the leading complication of HTN is pivotal for a better visual outcome. Increased blood pressure on its own cannot fully clarify the development of retinal alterations, therefore an additional pathogenetic mechanism, such as oxidative stress, might be inquired. The aim of the study was to evaluate the changes in the level of ischemia modified albumin (IMA) in the serum and tears of HR patients in order to establish the predictive value of IMA for the HR progression. Methods. Serum and tear samples for the measurement of IMA were collected from 90 patients detected primarily with HR, who were not taking any antihypertensive or other drug that could influence the results of the study, divided according to the Keith-Wagener classification into GI ‒ 36 patients with HR grade I, GII ‒ 35 with HR grade II and GIII ‒ 19 with HR grade III. Serum and tear IMA levels were assessed using the Co2+ binding method (Gudumac V. et al., 2009) and expressed as median and interquartile range. Kruskal-Wallis and Mann-Whitney nonparametric tests were used to compare the groups and the Spearman correlation coefficient was calculated (SPSS 23.0), with p<0.05 being statistically significant. Results. The groups showed a statistically significant difference in serum IMA (p=0.006), the values increasing in parallel with the progression of HR. The serum IMA level in GII increased compared to GI (+3%; 239.06 µM/L (IQR 75.58) vs 231.77 µM/L (IQR 104.09), p=1.00), as well as in GIII patients compared to GII (+17%; 277.67 µM/L (IQR 88.72) vs 239.06 µM/L (IQR 75.58), p=0.04). There were no differences in IMA content (p=0.160), between groups in the tears. No correlations were found between serum and tear IMA levels (p=0.134), but serum IMA showed a significant moderate strength, positive correlation with the degree of HR (r=0.307**, p=0.003). Conclusion. A progressive enhancement in serum IMA level as HR advanced was identified. Thereby, the results suggest the potential relevance of serum IMA as a sensitive and early biomarker useful for grading and optimal treatment of the patients with HR.

Introduction. Thiosemicarbazones represent a class of organic compounds with well-documented pharmacological properties, including antitumor, antimicrobial, and antiviral activities. Contemporary research highlights their role in modulating cellular redox equilibrium through antioxidant pathway regulation. The growing interest in copper-based coordination complexes with thiosemicarbazones is driven by the unique redox flexibility and high biocompatibility of copper ions, properties that underlie their potential in therapeutic and diagnostic applications. This investigation assessed the capacity of specific local bioactive thiosemicarbazones to impact the antioxidant system using in vitro methodologies. Material and methods. Peripheral blood samples from ten healthy volunteers were used to evaluate in vitro the influence of 10 copper-based coordination complexes with thiosemicarbazones at concentrations of 10.0 μM/L and 1.0 μM/L on antioxidant markers ‒ total antioxidant activity (via ABTS assay), total antioxidant capacity, antioxidant substance mass, and antioxidants total activity. Results. Data indicated that targeted copper-based coordination complexes with thiosemicarbazones affect general antioxidant markers. The study demonstrates that thiosemicarbazones exhibit concentration- and structure-dependent redox modulation, disclosing distinct mechanisms of action across three structural classes - benzothiazole, phenyl, and allyl thiosemicarbazone derivatives. Structural optimization (e.g., benzothiazole with methoxy groups) yields compounds like MG-22 that maintain redox equilibrium, while pro-oxidant variants (CMA-18) offer therapeutic potential through selective oxidative cytotoxicity. Conclusions. Copper-based coordination complexes with thiosemicarbazones represent a promising class of redox modulators with tunable biological effects. Their bidirectional activity, manifested by stimulation or inhibition of antioxidant mechanisms, confirms the potential of these derivatives as selective therapeutic agents. When these results are integrated in the context of personalized medicine, thiosemicarbazones become valuable candidates in the development of therapeutic strategies aimed at maintaining cellular homeostasis, especially in pathologies characterized by increased oxidative stress, such as cancer and neurodegenerative diseases.

Introduction. Arterial and venous thromboembolism is a disease with a high impact on morbidity and mortality. Their pathological mechanisms of aggregation directed by the clotting factors along with the variations in clinical manifestation are regarded to a high moiety of genetic polymorphisms along with a wide diversity of comorbidities. Material and methods. A comprehensive literature review was conducted, which included a total of 119 sources. Among these, 60 sources were systematically collected, while the remaining 59 sources were selected through non-systematic methods. Results. We have identified different treatment options that regard both the venous or arterial thromboembolism in contrast with numerous pathogenetic outcomes, population groups along with biomarkers that significantly modify the clinical aspects of the therapeutical and post-clinical treatment aspect. At the moment its diagnosis is continuously improving worldwide, taking into consideration a high diversity of experts’ opinions with a wide practical experience. Conclusions. Arterial and venous thromboembolisms are serious medical conditions that can be prevented and effectively managed with modern diagnostic and therapeutic techniques.

The objective was to determine the changes in SOD and catalase activity, markers of oxidative stress/antioxidant balance in serum and tear of patients with hypertensive retinopathy and to identify whether there was a correlation between their levels and HR degree of hypertensive retinopathy (HR). Material and Methods — 90 hypertensive patients were divided in three groups, according to the Keith-Wagener classification: GI-36, GII-35 and GIII-19. SOD was assessed using the Dubinina and Matyushin method and catalase according to Koroliuk, both in modification of Gudumac V. The results were presented by median and interquartile range. The groups were compared using Kruskal-Wallis and Mann-Whitney nonparametric tests, and the Spearman correlation coefficient was calculated (SPSS 23.0). Results — Showed a statistically significant difference of SOD in serum (p=0.035) and tear (p=0.027) between groups. SOD decreased from GI until GIII in serum (-8%, p=0.032) and tear (-16%, p=0.031). In addition, it showed a weak significant negative correlation with the HR degree both in serum (r=-0.246, p=0.019) and tear (r=-0.284, p=0.007), while the correlation attested between serum and tear SOD levels was significant moderate and positive (r=0.336, p=0.001). It was noted a significant catalase elevation in the tear (p=0.033). In serum it was not correlated with HR degree, while in tear showed a significant weak strength, positive correlation (r=0.261, p=0.013). No correlations were found between serum and tear catalase levels. Conclusion — A progressive significant decrease in SOD levels and a tendency to increase of catalase activity was identified as HR advanced both in serum and in tear. The enhancement in the severity of HR was correlated with decreased SOD activity in tear and serum and increased catalase level in tear.