From wild dogs (Lycaon pictus) in the Serengeti to tigers (Panthera tigris altaica) in the Russian Far East, canine distemper virus (CDV) has been repeatedly identified as a threat to wild carnivores. Between 2020 and 2022, six Indian leopards (P. pardus fusca) presented to Nepali authorities with fatal neurological disease, consistent with CDV. Here, we report the findings of a serosurvey of wild felids from Nepal. A total of 48 serum samples were tested, comprising 28 Bengal tigers (P. t. tigris) and 20 Indian leopards. Neutralizing antibodies were identified in three tigers and six leopards, equating to seroprevalences of 11% (CI: 2.8–29.3%, n = 28) and 30% (CI: 12.8–54.3%, n = 20), respectively. More than one-third of seropositive animals were symptomatic, and three died within a week of being sampled. The predation of domestic dogs (Canis lupus familiaris) has been posited as a potential route of infection. A comparison of existing diet studies revealed that while leopards in Nepal frequently predate on dogs, tigers do not, potentially supporting this hypothesis. However, further work, including molecular analyses, would be needed to confirm this.

Twelve cat species were recorded in Nepal including the largest, Tiger Panthera tigris, and the smallest, Rusty-spotted Cat Prionailurus rubiginosus. There is more research on the Panthera species than on small wild cats; consequently, the conservation status, distribution, and ecology of small cat species are poorly known. In this article, we report on the first photographic evidence of Clouded Leopard Neofelis nebulosa and Fishing Cat Prionailurus viverrinus in Parsa National Park in southern central Nepal during a camera trap survey targeted at the tiger between 2014 and 2016. There were only single detections of each species; this does not give enough information to establish distribution or conservation status of either of the species in Parsa National Park. Further targeted surveys are needed to establish the significance of this protected area for the conservation of these two species.

Canine distemper virus (CDV) is responsible for a highly contagious and often fatal neurological disease that affects various carnivores, including domestic dogs. In Nepal, recent reports of CDV exposure and illness in leopards (Panthera pardus) have raised concerns about the transmission of the virus among domestic dogs and wild carnivores. To investigate the genetic lineage and spread of CDV, our study utilized archived post-mortem samples from four leopards that exhibited clinical signs suggestive of canine distemper infection. These leopards were rescued in the Palpa, Dolakha, Kathmandu, and Parbat districts. Our phylogenetic analysis revealed that the CDV strains circulating among the leopards belong to the Asia-5 lineage, which is also prevalent among dogs and wild carnivores in Nepal and neighboring India. The genetic relatedness between the leopard CDV sequences and those from both dogs and other carnivores within the Asia-5 lineage suggests that leopards in Nepal may have acquired the virus from multiple sources, potentially facilitated by their generalist dietary habits preying on dogs and even mesocarnivores. Furthermore, we inspected specific amino acid substitution in the hemagglutinin gene of leopard CDV, which also suggests possible transmission from both domestic dogs and non-canid hosts, although further research is needed to draw definitive conclusions. Given the vulnerable state of the leopard population in Nepal, already threatened by poaching and retaliatory killing, the emergence of CDV as a potential novel threat is deeply concerning. Comprehensive surveillance studies are essential to understand the dynamics of CDV spillover and to develop informed interventions. Urgent measures, including vaccination programs and effective control of the dog population, are needed to mitigate the impact of this disease and safeguard the future of Nepal's leopards and other wild carnivores.

Koshi Tappu Wildlife Reserve (KTWR) has the last remaining Nepalese population of the Endangered Asiatic Wild Buffalo (Bubalus arnee Kerr, 1792). Individual animals protected inside KTWR may be of purely wild, domestic or hybrid origin, and the wild population is under potential threat due to habitat loss and genetic introgression from feral backcrosses. Identification of genetically pure wild individuals is important for identifying animals for translocation to other areas within their former range. In this study we have sequenced a highly variable 422bp region of the Cytochrome b gene of 36 animals, and added 61 published sequences of both River and Swamp Buffalo from Italy and some southern Asian countries including India. The haplotype diversities ranged from 0.286-0.589 with slightly higher diversities in domesticated individuals. The AMOVA analysis revealed that 97.217% of the genetic variation was contained within groups and 2.782% occurred among groups. An overall fixation index (FST) was found to be 0.02782 (p>0.05). Phylogenetic relationships derived through a reduced median network and maximum parsimony analyses reconfirmed the ancestral nature of the Wild Water Buffalo. Moreover, this study has reviewed recent achievements of molecular research in wild buffalo, assessed the technical capacities of research institutes in Nepal to conduct molecular research required for identifying pure wild individual in KTWR and more importantly initiated DNA bank and DNA sequence library of buffalos, which will enable an international collaboration for advanced molecular research in the future.